South Indian Journal of Biological Sciences

Seung Yeon Suh ¹, Thanislas Bastin Baskar ¹, Haeng Hoon Kim ², Naif Abdulla Al-Dhabi ², Sang Un Park ¹

Affiliations
1 Department of Crop Science, Chungnam National University, 99 Daehak-Ro, Yuseong-Gu, Daejeon 305-764, KR
2 Department of Well-being Resources, Sunchon National University, 413 Jungangno, Suncheon, Jeollanam-do, 540-742, KR

Abstract

Aloe species originated in Africa; they are known to have medicinal properties and are being used in various commercial products worldwide. Hence, mass production via plant tissue culture is common in this species. However, some species such as Aloe arborescens have a low regeneration rate, and hence, ethylene inhibitors have to be used to enhance their regeneration. This study aimed to use the meristem for the micro propagation of A. arborescens. We found that the addition of the growth regulator 6-benzylaminopurine (BAP), and not kinetin, improved the regeneration of A. arborescens. The highest shoot number (3.9 ± 0.6 per explant) and shoot length (2.7 ± 0.3 cm) were noted after treatment with 1 and 0.5 mg/L BAP, respectively. The ethylene inhibitor AgNO₃ (10 mg/L) increased shoot formation (5.6 ± 0.6 per explant) and growth (3.3 ± 0.4 cm). Our findings suggest that micro propagation of Aloe by using meristem could be a useful method for the mass production of various phytochemicals produced by Aloe plants.

Keywords

Ethylene Inhibitors, Organogenesis, Plant Regeneration, Aloe arborescens.

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