SMU Medical Journal

Open Access Open Access  Restricted Access Subscription Access

Biochemical Investigations on Lentinus squarrosulus (Mont.) Singer by Haemoagglutination Assay with Human Erythrocytes


Affiliations
1 Akal Group of Institutions, Mastuana Sahib, Sangrur-148001(Pb), India
 

Biochemical investigations on Lentinus squarrosulus(Mont.) Singer by haemoagglutination assay with human erythrocytes.

Haemagglutination was assayed using human type A, B, AB&O erythrocytes and lectin activity was expressed in terms of titre, defined as inverse of highest dilution causing visible haemagglutination.

The results achieved indicated that lectins present in the L. squarrosulus mycelium possess the capability to agglutinate all the four human blood groups. This property of mushroom lectins is of immense significance in pharmaceutical investigations.

It is apparent that L. squarrosulus can fit well in the menu of modern calorie conscious society. Its commercial production not only promises a strong food alternative for mushroom lovers with a potential to provide equally potent culinary option as is provided by edible species of Agaricus, Pleurotus, Volvariella, Lentinula, etc. but also carries nutraceutical relevance leading to human welfare.


Keywords

Lentinus squarrosulus (Mont.), Singer, Lectins, Haemoagglutination Assay.
User
Notifications
Font Size

  • Purkayastha, R. P. and Chandra, A.(1985) Manual of Indian Edible Mushrooms. Jagmander Book Agency, New Delhi.

  • Tewari, R. P. (2005) Mushroom, their role in nature and society. In: Frontiers in Mushroom Biotechnology (R. D Rai., R. C. Upadhyay and S. R. Sharma, eds.), NRCM, Chambaghat, Solan, pp. 1 - 8.

  • Sharma, S. R. (1997) Scope of specialty mushrooms in India. In: Advances in Mushroom Biology and Production. (R. D Rai, B. L. Dhar, and R. N. Verma, eds.) NRCM, Chambaghat, Solan, pp. 193 - 203.

  • Tewari, R. P. (2003) Cultivation of Milky Mushroom (Calocybe indica). In: Summer School on Emerging Areas of Mushroom Research and Production. (R. D Rai, R. C. Upadhyay and S. R. Sharma eds.), NRCM, Chambaghat, Solan, pp. 127 - 129.

  • Kirk, P. M., Cannon, P. F., Minter, P. F. and Stalpers, J. A. (2008) Ainsworth Bisby’s Dictionary of Fungi (10th ed.) CAB International Wallingford, Oxon, OX10, 8DE., UK.

  • Abbot, O., Okhuoya, J. A. and Akpaja, E. O. (2008) Growth of Lentinus squarrosulus (Mont.) Singer on saw dust of different tropical tree species. African Journal of Food Science. 3: 7 - 10.

  • Singh, R. S., Bhari, R. and Kaur, H. P. (2010) Mushroom lectins: Current status and future perspectives. Critical Reviews in Biotechnology, 30 (2): 99 – 126.

  • Sharon, N. and Lis, H. Lectins: (1972) Cell-agglutinating and sugar specific proteins.Science. 177: 949 - 959.

  • Goldstein, I. J., Hughes, R. C., (1980) Monsigny, M., Osawa, T. and Sharon, N. What should be called a lectin? Nature. 285: 66.

  • Maria, R. C. G. N., Novaes, L. C. G. and Taveira, V. C. (2007) Natural products from Agaricales medicinal mushrooms: Biology, nutritional properties and pharmacological effects on cancer. Revista Brasieleira de Cancerologia. 53 (4): 411 - 420..

  • Li, Y. R., Liu, Q. H., Wang, H. X. and Ng, T. B. (2008) A novel lectin with potent antitumor, mitogenic and HIV-1 reverse transcriptase inhibitory activities from the edible mushroom Pleurotus citrinopileatus. Biochem. Biophys Acta. 1780: pp. 51 - 57.

  • Parslew, R., Jones, K. T., Rhodes, J. M. and Sharpe, G. R. (1999) The antiproliferative effect of lectin from edible mushroom (Agaricus bisporus) on human keratinocytes: preliminary studies on its use in psoriasis. Br. J. Dermatol. 140 (1): 56 - 60.


Abstract Views: 248

PDF Views: 179




  • Biochemical Investigations on Lentinus squarrosulus (Mont.) Singer by Haemoagglutination Assay with Human Erythrocytes

Abstract Views: 248  |  PDF Views: 179

Authors

Rajinder Singh
Akal Group of Institutions, Mastuana Sahib, Sangrur-148001(Pb), India

Abstract


Biochemical investigations on Lentinus squarrosulus(Mont.) Singer by haemoagglutination assay with human erythrocytes.

Haemagglutination was assayed using human type A, B, AB&O erythrocytes and lectin activity was expressed in terms of titre, defined as inverse of highest dilution causing visible haemagglutination.

The results achieved indicated that lectins present in the L. squarrosulus mycelium possess the capability to agglutinate all the four human blood groups. This property of mushroom lectins is of immense significance in pharmaceutical investigations.

It is apparent that L. squarrosulus can fit well in the menu of modern calorie conscious society. Its commercial production not only promises a strong food alternative for mushroom lovers with a potential to provide equally potent culinary option as is provided by edible species of Agaricus, Pleurotus, Volvariella, Lentinula, etc. but also carries nutraceutical relevance leading to human welfare.


Keywords


Lentinus squarrosulus (Mont.), Singer, Lectins, Haemoagglutination Assay.

References