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Jenie, Ikhlas Muhammad
- The Comparison of Maximal Platelet Aggregation in the Presence of Disperse Primary and Monolayer Secondary HUVEC Exposed to Testosterone in High Glucose Medium
Abstract Views :500 |
PDF Views:156
Authors
Affiliations
1 Department of Physiology, Universitas Muhammadiyah Yogyakarta, ID
2 Department of Anatomy and Embryology, Universitas Gadjah Mada, Yogyakarta, ID
3 Department of Clinical Pathology, Universitas Gadjah Mada, Yogyakarta, ID
4 Department of Physiology, Universitas Gadjah Mada, Yogyakarta, ID
1 Department of Physiology, Universitas Muhammadiyah Yogyakarta, ID
2 Department of Anatomy and Embryology, Universitas Gadjah Mada, Yogyakarta, ID
3 Department of Clinical Pathology, Universitas Gadjah Mada, Yogyakarta, ID
4 Department of Physiology, Universitas Gadjah Mada, Yogyakarta, ID
Source
SMU Medical Journal, Vol 4, No 2 (2017), Pagination: 80-90Abstract
To explore in vitro models in examining the influence of testosterone (T) to platelet activation through endothelial cells in hyperglycemia condition. Disperse primary and monolayer secondary human umbilical vein endothelial cells (HUVEC) in high glucose (HG) medium were exposed to T. Maximal platelet aggregation in the presence of HUVEC was measured using turbidimetric method. Maximal platelet aggregation in the presence of disperse primary HUVEC exposed to 0, 1, 10, and 100 nM of T were 69.4%, 65.4%, 67.6%, and 62.05%, whereas of monolayer secondary HUVEC were 46.5%, 43.9%, 48.1%, and 37.45%, respectively. Maximal platelet aggregation was significantly lower (p = 0.0001) in disperse primary HUVEC than in monolayer secondary HUVEC. The two methods were moderately correlated (r = 0.06; p = 0.079) in terms of endothelial activation.Maximal platelet agregation in the presence of disperse primary HUVEC is higher than, but correlated to, of monolayer secondary HUVEC, which is exposed to T in HG medium.Keywords
Platelet Aggregation, Endothelial Cells, Testosterone, High Glucose, In Vitro.Full Text
References
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- Reaction Time in Healthy Female Subjects in Relation to Monthly Sexual Cycle
Abstract Views :461 |
PDF Views:170
Authors
Affiliations
1 Mlati Community Health Care, Tirtoadi, Mlati, Sinduadi, Sleman, Yogyakarta, ID
2 Department of Physiology, Universitas Muhammadiyah Yogyakarta, ID
1 Mlati Community Health Care, Tirtoadi, Mlati, Sinduadi, Sleman, Yogyakarta, ID
2 Department of Physiology, Universitas Muhammadiyah Yogyakarta, ID
Source
SMU Medical Journal, Vol 4, No 2 (2017), Pagination: 113-119Abstract
To examine whether mental process in female subjects is influenced by monthly sexual cycle. Reaction time (RT) was measured in twenty healthy female undergraduates, who had regular menstrual cycle, normal body mass index and normal hearing function, low anxiety level, and had no color blindness. Simple and choice RT to visual and auditory stimuli were measured using a response velocity measurement device. The RT measurements were done in day 4 of the cycle (menstruation phase) and day 7 (follicular phase) with the randomly assigned time order. Data were analyzed using paired t-test. Visual RT, auditory RT, and choice RT decreased significantly in follicular phase than during menstruation (p < 0.05). Mental process in female subjects is influenced by monthly sexual cycle.Keywords
Simple Reaction Time, Choice Reaction Time, Mental Process, Menstrual Cycle, Estrogen.Full Text
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