SMU Medical Journal

A. K. Jaiswal ¹, Sunil Kumar ², Jeevanjot Kaur ³, Jasbir Kaur ²

Affiliations
1 Department of Forensic Medicine and Toxicology, All India Institute of Medical Sciences, New Delhi-110029, IN
2 Department of Ocular Biochemistry, Dr. R. P. Centre for Ophthalmic Sciences, All India Institute of Medical Sciences, New Delhi- 110029, IN
3 Department of Biotechnology, Lovely Professional University, Phagwara, Punjab- 144402, IN

Abstract

2,4-Dichlorophenoxyacetic acid, more commonly referred to as 2,4-D, is an Organochlorus poison which is one of the most widely used herbicides. Several instrumental method like Gas liquid chromatography (GLC), High performance liquid chromatography (HPLC), UV-visible Spectrophotometer, etc., are available for the separation and identification of 2,4-D, but most of them are expensive and more time consuming. So, a relatively inexpensive and less time consuming method is presented for the separation of 2,4-D i.e. Thin Layer Chromatography (TLC). This method has several advantages over the other methods such as its low cost, fewer chemicals required simultaneous analysis of large and low amount of sample. 2,4-D was extracted from the blood and then it was identified by using 20 solvent systems. The spots developed on the TLC plates were developed using Iodine vapors.

Keywords

2,4-D, Organochloro, TLC, RF , Spraying Reagent Etc.

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