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Saxena, Jyoti
- Production of Protease from Soil Fungi by Submerged Fermentation
Abstract Views :565 |
PDF Views:172
Authors
Affiliations
1 Department of Bioscience and Biotechnology, Banasthali University, Rajasthan, IN
2 Department of Biochemical Engineering, Bipin Tripathi Kumaon Institute of Technology, Dwarahat, Uttrakhand, IN
1 Department of Bioscience and Biotechnology, Banasthali University, Rajasthan, IN
2 Department of Biochemical Engineering, Bipin Tripathi Kumaon Institute of Technology, Dwarahat, Uttrakhand, IN
Source
SMU Medical Journal, Vol 4, No 2 (2017), Pagination: 211-221Abstract
The demand of proteases is increasing regularly because of their numerous applications in the biotechnological industries. It promotes isolation and identification of potent protease producing fungi from soil samples. Therefore, the objective of the present investigation was to screen soil fungi in submerged fermentation (SmF) for detection of hyperproducer isolate and production of protease in SmF. Fungal strains from three diverse soil samples were isolated on potato dextrose agar (PDA) plates by serial dilution agar plate technique, purified by point inoculation and twelve isolates utilized for production of extracellular protease in SmF. Results were examined both in terms of protease activity and specific activity. Among all 12 fungal strains, isolate S2St1 (isolated from medicinal garden soil) exhibited maximum activity of protease (256.63 ± 3.18 U/ml/min) at 72 h of incubation. Isolate S2St1 was identified as potent producer of protease in SmF. Further this isolate can be used for production of large quantity of protease in SmF within very short time period of 3 days.Keywords
Proteases, Potato Dextrose Agar, Fungi, Soil Samples, Submerged Fermentation.Full Text
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- Optimization of Protease Production from Wild and Mutant Strains of Aspergillus sp. under Submerged Fermentation
Abstract Views :529 |
PDF Views:164
Authors
Affiliations
1 Department of Bioscience and Biotechnology, Banasthali University, Rajasthan, IN
2 Department of Biochemical Engineering, Bipin Tripathi Kumaon Institute of Technology, Dwarahat, Uttrakhand, IN
1 Department of Bioscience and Biotechnology, Banasthali University, Rajasthan, IN
2 Department of Biochemical Engineering, Bipin Tripathi Kumaon Institute of Technology, Dwarahat, Uttrakhand, IN
Source
SMU Medical Journal, Vol 4, No 2 (2017), Pagination: 234-247Abstract
Proteases are one of the most significant groups of commercial enzymes and contribute 60% of the world enzyme market. The choice of proteases is the microbial proteases due to their versatility, stability and distinctive properties. Today, microbial proteases are utilized in various industries viz., meat processing, ripening of cheese, detergents, textile, recovery of silver from photographic image etc. Therefore, the present study was undertaken to increase the protease production from wild and mutagenic strain of Aspergillus sp. Wild and mutagenic strain of proteolytic soil fungus Aspergillus sp. was used for optimization study. Different parameters (nitrogen, carbon sources, temperature, pH and incubation time) were optimized in submerged fermentation (SmF) for enhanced protease production. Wild strain demonstrated highest protease activity at pH 10.0 after 48 h of incubation at 37 °C in the medium containing glucose as carbon source and yeast extract as nitrogen source whereas optimum protease activity from mutagenic strain was found at pH 10.0 after 94 h of incubation at 37 °C in the medium containing fructose as carbon source and peptone as nitrogen source. Protease production was increased from wild and mutagenic strain of Aspergillus sp. after parameters optimization in SmF.Keywords
Optimization, Protease Activity, Aspergillus sp., Carbon Source, Temperature, pH.Full Text
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