Refine your search
Collections
Co-Authors
Journals
Year
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z All
Negi, Shreya
- Production of Protease from Soil Fungi by Submerged Fermentation
Abstract Views :550 |
PDF Views:168
Authors
Affiliations
1 Department of Bioscience and Biotechnology, Banasthali University, Rajasthan, IN
2 Department of Biochemical Engineering, Bipin Tripathi Kumaon Institute of Technology, Dwarahat, Uttrakhand, IN
1 Department of Bioscience and Biotechnology, Banasthali University, Rajasthan, IN
2 Department of Biochemical Engineering, Bipin Tripathi Kumaon Institute of Technology, Dwarahat, Uttrakhand, IN
Source
SMU Medical Journal, Vol 4, No 2 (2017), Pagination: 211-221Abstract
The demand of proteases is increasing regularly because of their numerous applications in the biotechnological industries. It promotes isolation and identification of potent protease producing fungi from soil samples. Therefore, the objective of the present investigation was to screen soil fungi in submerged fermentation (SmF) for detection of hyperproducer isolate and production of protease in SmF. Fungal strains from three diverse soil samples were isolated on potato dextrose agar (PDA) plates by serial dilution agar plate technique, purified by point inoculation and twelve isolates utilized for production of extracellular protease in SmF. Results were examined both in terms of protease activity and specific activity. Among all 12 fungal strains, isolate S2St1 (isolated from medicinal garden soil) exhibited maximum activity of protease (256.63 ± 3.18 U/ml/min) at 72 h of incubation. Isolate S2St1 was identified as potent producer of protease in SmF. Further this isolate can be used for production of large quantity of protease in SmF within very short time period of 3 days.Keywords
Proteases, Potato Dextrose Agar, Fungi, Soil Samples, Submerged Fermentation.References
- Gupta, R., Beg, QK. and Lorenz, P (2002) Bacterial alkaline proteases: molecular approaches and industrial applications. Appl Microbiol Biotechnol. 59, 15-32.
- Lin, Y. and Tanaka, S (2006) Ethanol fermentation from biomass resources: current state and prospects. Appl. Microbiol Biotechnol. 69, 627-642.
- Brijwani, K., Oberoi, HS. and Vadlani, PV (2010) Production of a cellulolytic enzyme system in mixed-culture solid-state fermentation of soybean hulls supplemented with wheat bran. Process Biochem. 45, 120-128.
- Krem, MM. and Di Cera, E (2001) Molecular markers of serine protease evolution. The EMBO Journal. 20, 3036-3045.
- da silva, RR., Caetano, RC., Okamoto, DN., de Oliveira, LC., Bertolin, TC., Juliano, MA., de Oliveira, AH., Rosae, JC. and Cabral, H (2014) The identification and biochemical properties of the catalytic specificity of a serine peptidase secreted by Aspergillus fumigatus Fresenius. Protein Pept Lett. 21, 663-671.
- Halabi, N., Rivoire, O., Leibler, S., and Ranganathan, R (2009) Protein sectors: evolutionary units of three-dimensional structure. Cell. 138, 774-786.
- Souza, PM., Bittencourt, MLA., Caprara, CC., Freitas, M., Almeida, RPC., Silveira, D., Fonseca, YM., Filho, EXF., Junior, AP., and Magalhaes, PO (2015) A biotechnology perspective of fungal proteases. Braz J Microbiol. 46, 337-346.
- Saran, S., Isar, J. and Saxena, RK (2007) A modified method for the detection of microbial proteases on agar plates using tannic acid. J Biochem Biophys Methods. 70, 697-699.
- Zambare, V., Nilegaonkar, S. and Kanekar, PA (2011) Novel extracellular protease from Pseudomonas aeruginosa MCM B-327: enzyme production and its partial characterization. N Biotechnol. 28, 173-181.
- Anitha, TS. and Palanivelu, P (2013) Purification and characterization of an extracellular keratinolytic protease from a new isolate of Aspergillus parasiticus. Protein Expr Purif. 88, 214-220.
- Sandhya, C., Sumantha, A., Szakacs, G. and Pandey, A (2005) Comparative evaluation of neutral protease production by Aspergillus oryzae in submerged and solid-state fermentation. Process Biochem. 40, 2689-2694.
- Edwinoliver, NG., Thirunavukarasu, K., Naidu, RB., Gowthaman, MK., Kambe, TN. and Kamini, NR (2010) Scale up of a novel tri-substrate fermentation for enhanced production of Aspergillus niger lipase for tallow hydrolysis. Bioresour Technol. 101, 6791-6796.
- Waksman, SA (1922) A Method for Counting the Number of Fungi in the Soil. J Bacteriol. 7, 339-341.
- Sharma, AK., Sharma, V., Saxena, J., Yadav, B., Alam, A. and Prakash, A (2015) Effect of culture conditions on protease production and activity of protease from soil borne fungi. International Journal of Scientific Research in Environmental Sciences. 3, 0411-0419.
- Sharma, AK., Sharma, V., Saxena, J., Yadav, B., Alam, A. and Prakash, A (2015) Isolation and screening of extracellular protease enzyme from bacterial and fungal isolates of soil. International Journal of Scientific Research in Environmental Sciences. 3, 0334-0340.
- Josephine, FS., Ramya, VS., Neelam, D., Suresh, BG., Siddalingeshwara, KG., Venugopal, N. and Vishwanatha, TJ (2012) Isolation, production and characterization of protease from Bacillus sp. Isolated from soil sample. J Microbiol Biotechnol Res. 2, 163-168.
- Lowry, OH, Rosenbrough, NJ., Farr, AL. and Randall, A (1951) Protein measurement with the folin phenol reagent. J Biol Chem. 193, 265-275.
- Tsuchida, O., Yamagota, Y., Ishizuka, J., Arai, J., Yamada, J., Takeuchi, M. and Ichishima, E (1986) An alkaline protease of an alkalophilic Bacillus sp. Curr. Microbiol.14, 7-12.
- Yezli, W., Zebboudj, N., Karkachi, NE., Kihal, M. and Henni, JE (2015) Influence of two substrates (casein and glucose) on mycelia growth and dosage of proteolytic activity of Fusarium oxysporum f. sp. albedinis (Foa). Int J Biosc. 6, 115-125.
- Anand, K (2016) Fungal Protease Production by Aspergillus niger and Aspergillus flavus Using Rice Bran as the Substrate. Acad J Agric Res. 4, 333-338.
- Oseni, OA (2011) Production of Microbial Protease from Selected Soil Fungal Isolates. Nig J Biotech. 23, 28-34.
- Ali, SS. and Vidhale, NN (2013) Protease Production by Fusarium oxysporum in SolidState Fermentation Using Rice Bran. Am J Microbiol Res. 1, 45-47.
- Irfan, M., Rauf, A., Syed, Q., Nadeem, M. and Baig, S (2011) Exploitation of Different Agro-residues for Acid protease Production by Rhizopus sp. in Koji Fermentation. Int J Agro Vet Med Sci. 5, 43-53.
- Kamath, P., Subrahmanyam, VM., Rao, JV. and Raj, PV (2010) Optimization of Cultural Conditions for Protease Production by a Fungal Species. Indian J Pharm Sci. 72, 161-166.
- Muthulakshmi, C., Duraisamy, G., Dugganaboyana, G. K., Ganesan, R., Manokaran, K. and Chandrasekar, U (2011) Production, Purification and Characterization of Protease by Aspergillus flavus under Solid State Fermentation. Jordan J Biol Sci. 4, 137-148.
- Optimization of Protease Production from Wild and Mutant Strains of Aspergillus sp. under Submerged Fermentation
Abstract Views :509 |
PDF Views:159
Authors
Affiliations
1 Department of Bioscience and Biotechnology, Banasthali University, Rajasthan, IN
2 Department of Biochemical Engineering, Bipin Tripathi Kumaon Institute of Technology, Dwarahat, Uttrakhand, IN
1 Department of Bioscience and Biotechnology, Banasthali University, Rajasthan, IN
2 Department of Biochemical Engineering, Bipin Tripathi Kumaon Institute of Technology, Dwarahat, Uttrakhand, IN
Source
SMU Medical Journal, Vol 4, No 2 (2017), Pagination: 234-247Abstract
Proteases are one of the most significant groups of commercial enzymes and contribute 60% of the world enzyme market. The choice of proteases is the microbial proteases due to their versatility, stability and distinctive properties. Today, microbial proteases are utilized in various industries viz., meat processing, ripening of cheese, detergents, textile, recovery of silver from photographic image etc. Therefore, the present study was undertaken to increase the protease production from wild and mutagenic strain of Aspergillus sp. Wild and mutagenic strain of proteolytic soil fungus Aspergillus sp. was used for optimization study. Different parameters (nitrogen, carbon sources, temperature, pH and incubation time) were optimized in submerged fermentation (SmF) for enhanced protease production. Wild strain demonstrated highest protease activity at pH 10.0 after 48 h of incubation at 37 °C in the medium containing glucose as carbon source and yeast extract as nitrogen source whereas optimum protease activity from mutagenic strain was found at pH 10.0 after 94 h of incubation at 37 °C in the medium containing fructose as carbon source and peptone as nitrogen source. Protease production was increased from wild and mutagenic strain of Aspergillus sp. after parameters optimization in SmF.Keywords
Optimization, Protease Activity, Aspergillus sp., Carbon Source, Temperature, pH.References
- Negi, S. and Banerjee, R (2006) Optimization of amylase and protease production from Aspergillus awamori in single bioreactor through EVOP factorial design technique. Food Technol Biotechnol. 44, 257-261.
- Rani, K., Rana, R. and Datt, S (2012) Review on latest overview of proteases. Int J Curr Life Sci 2, 12-18.
- Rocha, MV., Romanini, D., Nerli, BB. and Tubio, G (2012) Pancreatic serine protease extraction by affinity partition using a free triazine dye. Int J Biol Macromol. 50, 303-309.
- Gupta, R., Beg, QK. and Lorenz, P (2002) Bacterial alkaline proteases: molecular approaches and industrial applications. Appl Microbiol Biotechnol. 59:15-32.
- Rao, MB., Tanksale, AM., Ghatge, MS. and Deshpande, VV (1998) Molecular and biotechnological aspects of microbial proteases. Microbiol Mol Biol Rev. 62, 597-635.
- Nirmal, NP., Shankar, S. and Laxman, RS (2011) Fungal proteases: an overview. Int J Biotech Biosci. 1, 1-40.
- Laxman, RS., Sonawane, AP., More, SV., Rao, BS., Rele, MV., Jogdand, VV., Deshpande, VV. and Rao, MB (2005) Optimization and scale up of production of alkaline protease from Conidiobolus coronatus. Process Biochem. 40, 3152-3158.
- Shankar, S., Rao, M. and Laxman, SR (2011) Purification and characterization of an alkaline protease by a new strain of Beauveria sp. Process Biochem. 46, 579-585.
- Wu, TY., Mohammad, AW., Jahim, JM. and Anuar, N (2006) Investigations on protease production by a wild-type Aspergillus terreus strain using diluted retentate of pre-filtered palm oil mill effluent (POME) as substrate. Enzyme Microb Tech. 39, 1223-1229.
- Farley, PC. and Ikasari, L (1992) Regulation of the secretion of Rhizopus Oligosporus extracellular carboxyl proteinase. J Gen Microbiol. 138, 2539-2544.
- Fan-Ching, Y. and Lin, IH (1998) Production of acid protease using thin stillage from a rice-spirit distillery by Aspergillus niger. Enzyme Microb Technol. 23, 397-402.
- Chrzanowska, J., Kolaczkowska, M. and Polanowski, A (1993) Production of exocellular proteolytic enzymes by various species of Penicillium. Enzyme Microb Technol. 15, 140-143.
- Aleksieva, P. and Peeva, L (2000) Investigation of acid proteinase biosynthesis by the fungus Humicola lutea 120-5 in an airlift bioreactor. Enzyme Microb Technol. 26, 402-405.
- Lasure, LL (1980) Regulation of extracellular acid protease in Mucor miehei. Mycologia, 72, 483-493.
- Rao, MB., Tanksale, AM., Ghatge, MS. and Deshpande, VV (1998) Molecular and biotechnological aspects of microbial proteases. Microbiol Mol Biol Rev. 62, 597-635.
- Tsuchida, O., Yamagota, Y., Ishizuka, J., Arai, J., Yamada, J., Takeuchi, M. and Ichishima, E (1986) An alkaline protease of an alkalophilic Bacillus sp. Curr Microbiol. 14, 7-12.
- Rajput, K., Chanyal, S. and Agrawal, PK. Optimization of protease production by endophytic fungus, Alternaria alternata isolated from gymnosperm tree- cupressus torulosa d.don. World J Pharma Pharm Sci. 5, 1034-1054, 2016.
- Srinubabu, G., Lokeswari, N. and Jayaraju, K (2007) Screening of Nutritional Parameters for the Production of Protease from Aspergillus Oryzae. E-Journal of Chemistry. 4, 208-215.
- Venkat Kumar, S., Rao, A. and Nazareth, JR (2015) Screening, media optimization and partial purification of protease by Trichosporon japonicum vitvk1. International Journal of Pharmacy and Pharmaceutical Sciences. 7, 187-191.
- Muthukrishnan, S. and Mukilarasi, K (2016) Industrial Important Protease Screening and Optimization from Micro-Fungal Isoltaes of Ayyanar Falls Forest Samples, Rajapalalyam. World Appl Sci J. 34, 343-347.
- Sharma, AK., Sharma, V., Saxena, J., Yadav, B., Alam, A. and Prakash, A. (2015) Effect of Culture Conditions on Protease Production and Activity of Protease from Soil Borne Fungi. International Journal of Scientific Research in Environmental Science. 3, 0411-0419.
- Zaferanloo, B., Quang, TD., Daumoo, S., Ghorbani, MM. and Palombo, EA (2014) Optimization of protease production by endophytic fungus, Alternaria alternata, isolated from an Australian native plant. World J Microbiol Biotechnol. 30, 1755-1762.
- Milala, MA., Jatau, IA. and Abdulrahman, AA (2016) Production and Optimization of Protease from Aspergillus niger and Bacillus subtilis using Response Surface Methodology. IOSR J Biotechnol Biochem. 2, 01-07.