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Ocular Squamous Cell Carcinoma in Holstein Cows From the South of Brazil


Affiliations
1 Graduate School Program in Veterinary Sciences (PPGCV-UFPR), Federal University of Parana, Rua dos Funcionarios, 1540, 80035-050, Juveve, Curitiba-PR, Brazil
2 Veterinary Diagnostic Laboratory, 4125 Beaumont RD BLDG 0215, Room 152A, Lansing, MI 48910, United States
 

Aim: The aim of this study was to investigate 10 cases of bovine ocular squamous cell carcinoma (OSCC) diagnosed in Holstein or Holstein-crosses cows.
Materials and Methods: The investigation was performed exclusively in OSCC cases diagnosed in the State of Parana and Santa Catarina. A combination of two previously existing histopathological classifications systems was used. The tissue samples were tested for immunoexpression of p53 and p16 and polymerase chain reaction (PCR) for bovine herpesvirus and papillomavirus.
Results: A positive correlation between number of mitotic figures and tissue invasion was found. Anaplasia parameters did not correlate well with tumor invasion of deeper tissues and mitotic counts. Six of 10 OSCC cases were in animals with heavily pigmented eyes. Immunoexpression of p53 and p16 was observed in 3 cases each. Bovine herpesvirus and papillomavirus were not detected by PCR.
Conclusions: Our results indicate that OSCC occurrence is most likely multifactorial with genetic, phenotypic, and environmental influences contributing to the pathogenesis of the disease.

Keywords

Bovine, Histologic Classification, Ophthalmology, P16, P53, Tumor Behavior.
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  • Ocular Squamous Cell Carcinoma in Holstein Cows From the South of Brazil

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Authors

Gabrielle A. Fornazari
Graduate School Program in Veterinary Sciences (PPGCV-UFPR), Federal University of Parana, Rua dos Funcionarios, 1540, 80035-050, Juveve, Curitiba-PR, Brazil
Juliana Kravetz
Graduate School Program in Veterinary Sciences (PPGCV-UFPR), Federal University of Parana, Rua dos Funcionarios, 1540, 80035-050, Juveve, Curitiba-PR, Brazil
Matti Kiupel
Veterinary Diagnostic Laboratory, 4125 Beaumont RD BLDG 0215, Room 152A, Lansing, MI 48910, United States
Dodd Sledge
Veterinary Diagnostic Laboratory, 4125 Beaumont RD BLDG 0215, Room 152A, Lansing, MI 48910, United States
Ivan Roque De Barros Filho
Graduate School Program in Veterinary Sciences (PPGCV-UFPR), Federal University of Parana, Rua dos Funcionarios, 1540, 80035-050, Juveve, Curitiba-PR, Brazil
Fabiano Montiani-Ferreira
Graduate School Program in Veterinary Sciences (PPGCV-UFPR), Federal University of Parana, Rua dos Funcionarios, 1540, 80035-050, Juveve, Curitiba-PR, Brazil

Abstract


Aim: The aim of this study was to investigate 10 cases of bovine ocular squamous cell carcinoma (OSCC) diagnosed in Holstein or Holstein-crosses cows.
Materials and Methods: The investigation was performed exclusively in OSCC cases diagnosed in the State of Parana and Santa Catarina. A combination of two previously existing histopathological classifications systems was used. The tissue samples were tested for immunoexpression of p53 and p16 and polymerase chain reaction (PCR) for bovine herpesvirus and papillomavirus.
Results: A positive correlation between number of mitotic figures and tissue invasion was found. Anaplasia parameters did not correlate well with tumor invasion of deeper tissues and mitotic counts. Six of 10 OSCC cases were in animals with heavily pigmented eyes. Immunoexpression of p53 and p16 was observed in 3 cases each. Bovine herpesvirus and papillomavirus were not detected by PCR.
Conclusions: Our results indicate that OSCC occurrence is most likely multifactorial with genetic, phenotypic, and environmental influences contributing to the pathogenesis of the disease.

Keywords


Bovine, Histologic Classification, Ophthalmology, P16, P53, Tumor Behavior.