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Prevalence of Carrier State Theileriosis in Lactating Cows


Affiliations
1 Department of Epidemiology & Preventive Medicine, College of Veterinary Science & Animal Husbandry, Orissa University of Agriculture and Technology, Bhubaneswar-751003, Odisha, India
2 Centre for Wildlife Health, College of Veterinary Science & Animal Husbandry, Orissa University of Agriculture and Technology, Bhubaneswar-751003, Odisha, India
3 ICMR-Regional Medical Research Centre, Department of Health Research, Ministry of Health & Family Welfare, Government of India, Bhubaneswar-751023, Odisha, India
 

Aim: The objective of this study was to examine the carrier status of theileriosis among apparently healthy cross-bred jersey cattle population of Odisha using conventional blood smear examination and polymerase chain reaction (PCR).
Materials and Methods: A total of 34 apparently healthy cross-bred Jersey lactating cows were considered in this study. Blood samples were subjected to microscopic examination after staining with Giemsa stain and PCR based molecular diagnosis using two sets of primer, i.e., N516/N517 and TorF1/TorF2 specific for Theileria annulata and Theileria orientalis, respectively.
Results: Examination of blood samples revealed presence of theileria parasites to a magnitude of 20.59% for T. annulata, 8.82% for T. orientalis, and 2.94% for both.
Conclusion: Molecular diagnosis was found to be much more sensitive than conventional method for diagnosis of theileriosis. T. annulata was found to be the predominant species affecting the exotic cattle. T. orientalis was detected in apparently healthy cows.

Keywords

Bovine Theileriosis, Carrier State, Polymerase Chain Reaction, Theileria annulata, Theileria orientalis.
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  • Prevalence of Carrier State Theileriosis in Lactating Cows

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Authors

Niranjana Sahoo
Department of Epidemiology & Preventive Medicine, College of Veterinary Science & Animal Husbandry, Orissa University of Agriculture and Technology, Bhubaneswar-751003, Odisha, India
Bikash Kumar Behera
Centre for Wildlife Health, College of Veterinary Science & Animal Husbandry, Orissa University of Agriculture and Technology, Bhubaneswar-751003, Odisha, India
Hemant Kumar Khuntia
ICMR-Regional Medical Research Centre, Department of Health Research, Ministry of Health & Family Welfare, Government of India, Bhubaneswar-751023, Odisha, India
Manojita Dash
Centre for Wildlife Health, College of Veterinary Science & Animal Husbandry, Orissa University of Agriculture and Technology, Bhubaneswar-751003, Odisha, India

Abstract


Aim: The objective of this study was to examine the carrier status of theileriosis among apparently healthy cross-bred jersey cattle population of Odisha using conventional blood smear examination and polymerase chain reaction (PCR).
Materials and Methods: A total of 34 apparently healthy cross-bred Jersey lactating cows were considered in this study. Blood samples were subjected to microscopic examination after staining with Giemsa stain and PCR based molecular diagnosis using two sets of primer, i.e., N516/N517 and TorF1/TorF2 specific for Theileria annulata and Theileria orientalis, respectively.
Results: Examination of blood samples revealed presence of theileria parasites to a magnitude of 20.59% for T. annulata, 8.82% for T. orientalis, and 2.94% for both.
Conclusion: Molecular diagnosis was found to be much more sensitive than conventional method for diagnosis of theileriosis. T. annulata was found to be the predominant species affecting the exotic cattle. T. orientalis was detected in apparently healthy cows.

Keywords


Bovine Theileriosis, Carrier State, Polymerase Chain Reaction, Theileria annulata, Theileria orientalis.