Veterinary World

Open Access Open Access  Restricted Access Subscription Access

Protective Efficacy of Combined Trivalent Inactivated ISA 71 Oil Adjuvant Vaccine Against Avian Influenza Virus Subtypes (H9N2 And H5N1) and Newcastle Disease Virus


Affiliations
1 Department of Poultry Vaccines, Production Unit Veterinary Serum and Vaccine Research Institute, Abbasia-11759, Egypt
2 Department of Virology, Cairo University, Giza-12211, Egypt
 

Aim: The objective of the present study was to prepare a trivalent inactivated vaccine of Newcastle disease virus (NDV), H5N1, and H9N2 viruses.
Materials and Methods: Three monovalent and a trivalent vaccines were prepared by emulsifying inactivated NDV (LaSota strain), reassortant H5N1, and H9N2 viruses with Montanide ISA 71 oil adjuvant. Parameters used for evaluation of the efficacy of the prepared vaccines in specific pathogen-free chickens were cellular immunity assays (blastogenesis, interferon gamma, interleukin 1 [IL1], and IL6), humoral immunity by hemagglutination inhibition, protection percentage, and shedding.
Results: A single immunization with trivalent vaccine-enhanced cell-mediated immunity as well as humoral immune response with 90% protection against challenges with highly pathogenic avian influenza (HPAI) H5N1 and low pathogenic (LP) avian influenza H9N2 viruses with 100% protection after challenge with NDV.
Conclusion: Development and evaluation of the trivalent vaccine in the study reported the success in preparation of a potent and efficacious trivalent vaccine which is a promising approach for controlling HPAI H5N1, LP H9N2, and ND viral infections.

Keywords

Avian Influenza, Immunization, Newcastle Disease Virus, Shedding, Trivalent Vaccine.
User
Notifications
Font Size

Abstract Views: 143

PDF Views: 0




  • Protective Efficacy of Combined Trivalent Inactivated ISA 71 Oil Adjuvant Vaccine Against Avian Influenza Virus Subtypes (H9N2 And H5N1) and Newcastle Disease Virus

Abstract Views: 143  |  PDF Views: 0

Authors

Zeinab Mohamed Ali
Department of Poultry Vaccines, Production Unit Veterinary Serum and Vaccine Research Institute, Abbasia-11759, Egypt
Mervat Abd El Monaem Hassan
Department of Poultry Vaccines, Production Unit Veterinary Serum and Vaccine Research Institute, Abbasia-11759, Egypt
Hussein Ali Hussein
Department of Virology, Cairo University, Giza-12211, Egypt
Basem Mohamed Ahmed
Department of Virology, Cairo University, Giza-12211, Egypt
Ahmed Abd El-Ghany El Sanousi
Department of Virology, Cairo University, Giza-12211, Egypt

Abstract


Aim: The objective of the present study was to prepare a trivalent inactivated vaccine of Newcastle disease virus (NDV), H5N1, and H9N2 viruses.
Materials and Methods: Three monovalent and a trivalent vaccines were prepared by emulsifying inactivated NDV (LaSota strain), reassortant H5N1, and H9N2 viruses with Montanide ISA 71 oil adjuvant. Parameters used for evaluation of the efficacy of the prepared vaccines in specific pathogen-free chickens were cellular immunity assays (blastogenesis, interferon gamma, interleukin 1 [IL1], and IL6), humoral immunity by hemagglutination inhibition, protection percentage, and shedding.
Results: A single immunization with trivalent vaccine-enhanced cell-mediated immunity as well as humoral immune response with 90% protection against challenges with highly pathogenic avian influenza (HPAI) H5N1 and low pathogenic (LP) avian influenza H9N2 viruses with 100% protection after challenge with NDV.
Conclusion: Development and evaluation of the trivalent vaccine in the study reported the success in preparation of a potent and efficacious trivalent vaccine which is a promising approach for controlling HPAI H5N1, LP H9N2, and ND viral infections.

Keywords


Avian Influenza, Immunization, Newcastle Disease Virus, Shedding, Trivalent Vaccine.