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Molecular Screening for Hemotropic Mycoplasmas in Captive Barbary Sheep (Ammotragus lervia) in Southern Brazil


Affiliations
1 Department of Sanitary Engineering, Engineering and Exact Sciences Center, Universidade Estadual Do Oeste Do Parana, Foz Do Iguacu, PR 85870-650, Brazil
2 Department of Preventive Veterinary Medicine, Universidade Estadual De Londrina, Londrina, PR 86057-970, Brazil
3 Department of Veterinary Medicine, Universidade Federal Do Parana, Curitiba, PR 80035-050, Brazil
 

Aim: This study is part of an active surveillance program for monitoring animal health status in endangered species, and was conducted to screen captive Barbary sheep (Ammotragus lervia) for hemoplasma infection.
Materials and Methods: A total of 12 blood samples were collected, DNA extracted and further tested by a pan-hemoplasma polymerase chain reaction protocol.
Results: Animals were clinically healthy and not infested by ectoparasites. Although housekeeping gene DNA was successfully amplified, all the Barbary sheep samples tested negative for Mycoplasma sp.
Conclusion: Notwithstanding the negative results, molecular pathogen surveys on Barbary sheep and other exotic wild mammals may provide insights regarding infection of endangered species caused by captivity stress in association with exposure to new pathogens worldwide.

Keywords

Aoudads, Eperythrozoonosis, Hemolytic Anemia, Hemoplasmas.
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  • Molecular Screening for Hemotropic Mycoplasmas in Captive Barbary Sheep (Ammotragus lervia) in Southern Brazil

Abstract Views: 193  |  PDF Views: 0

Authors

Leonilda C. Santos
Department of Sanitary Engineering, Engineering and Exact Sciences Center, Universidade Estadual Do Oeste Do Parana, Foz Do Iguacu, PR 85870-650, Brazil
Odilon Vidotto
Department of Preventive Veterinary Medicine, Universidade Estadual De Londrina, Londrina, PR 86057-970, Brazil
Vivien M. Morikawa
Department of Veterinary Medicine, Universidade Federal Do Parana, Curitiba, PR 80035-050, Brazil
Nelson J. R. Santos
Department of Preventive Veterinary Medicine, Universidade Estadual De Londrina, Londrina, PR 86057-970, Brazil
Thallitha S. W. J. Vieira
Department of Veterinary Medicine, Universidade Federal Do Parana, Curitiba, PR 80035-050, Brazil
Ivan R. Barros Filho
Department of Veterinary Medicine, Universidade Federal Do Parana, Curitiba, PR 80035-050, Brazil
Rafael F. C. Vieira
Department of Veterinary Medicine, Universidade Federal Do Parana, Curitiba, PR 80035-050, Brazil
Alexander W. Biondo
Department of Veterinary Medicine, Universidade Federal Do Parana, Curitiba, PR 80035-050, Brazil

Abstract


Aim: This study is part of an active surveillance program for monitoring animal health status in endangered species, and was conducted to screen captive Barbary sheep (Ammotragus lervia) for hemoplasma infection.
Materials and Methods: A total of 12 blood samples were collected, DNA extracted and further tested by a pan-hemoplasma polymerase chain reaction protocol.
Results: Animals were clinically healthy and not infested by ectoparasites. Although housekeeping gene DNA was successfully amplified, all the Barbary sheep samples tested negative for Mycoplasma sp.
Conclusion: Notwithstanding the negative results, molecular pathogen surveys on Barbary sheep and other exotic wild mammals may provide insights regarding infection of endangered species caused by captivity stress in association with exposure to new pathogens worldwide.

Keywords


Aoudads, Eperythrozoonosis, Hemolytic Anemia, Hemoplasmas.