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Hemogram Responses in Goats Toward Challenged With Corynebacterium pseudotuberculosis and its Immunogen Mycolic Acids


Affiliations
1 Department of Veterinary Medicine, Thamar University, Dhamar, Yemen
2 Department of Veterinary Clinical Studies, Research Center for Ruminant Disease, Universiti Putra Malaysia, Serdang-43400, Selangor, Malaysia
3 Department of Veterinary Clinical Studies, Universiti Putra Malaysia, Serdang-43400, Selangor, Malaysia
4 Department of Veterinary Pathology and Microbiology, Universiti Putra Malaysia, Serdang-43400, Selangor, Malaysia
5 Department of Veterinary Public Health and Preventive Medicine, University of Maiduguri, Nigeria
6 Department of Animal Reproduction, University of Agriculture, Water and Marine Sciences, Uthal, Baluchistan, Pakistan
7 Department of Internal and Preventive Medicine, University of Basra, Basra, Iraq
 

Aim: This study was conducted to analyze the changes in blood profile of goats inoculated with Corynebacterium pseudotuberculosis and its immunogen mycolic acid (MA) extract.
Materials and Methods: A total of 12 clinically healthy crossbred Boer female goats were divided into three groups; A, B and C (4 goats each per group). Group A was inoculated with 2 ml sterile phosphate buffered saline via intradermal route as the negative control group whilst Group B was inoculated with 2 ml of MA extract (1 g/ml) intradermally and Group C was then inoculated with 2 ml (1×109) colony forming unit of active C. pseudotuberculosis intradermally. Blood sample was collected aseptically from the jugular vein periodically for complete blood count (CBC) analysis throughout the experimental period (3 months).
Result: A significant decrease (p<0.05) was observed in red blood cells, hemoglobin (Hb), packed cell volume, mean corpuscular volume and mean corpuscular Hb concentration in Groups B and C as compared to the control while WBCs, neutrophil, lymphocyte and basophil showed a significant increase (p<0.05) as compared to the control.
Conclusion: The inoculation of C. pseudotuberculosis and MA resulted in a significant change in the CBC, thereby, indicating that MA has a role in caseous lymphadenitis pathogenesis.

Keywords

Complete Blood Count, Corynebacterium pseudotuberculosis, Goat, Hematology, Mycolic Acid.
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  • Hemogram Responses in Goats Toward Challenged With Corynebacterium pseudotuberculosis and its Immunogen Mycolic Acids

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Authors

Mohammed Naji Odhah
Department of Veterinary Medicine, Thamar University, Dhamar, Yemen
Faez Firdaus Jesse Abdullah
Department of Veterinary Clinical Studies, Research Center for Ruminant Disease, Universiti Putra Malaysia, Serdang-43400, Selangor, Malaysia
Abd Wahid Haron
Department of Veterinary Clinical Studies, Universiti Putra Malaysia, Serdang-43400, Selangor, Malaysia
Mohd. Azmi Mohd. Lila
Department of Veterinary Pathology and Microbiology, Universiti Putra Malaysia, Serdang-43400, Selangor, Malaysia
Mohd. Zamri‑Saad
Department of Veterinary Pathology and Microbiology, Universiti Putra Malaysia, Serdang-43400, Selangor, Malaysia
Zaid Khuder
Department of Veterinary Clinical Studies, Universiti Putra Malaysia, Serdang-43400, Selangor, Malaysia
Idris Umar Hambali
Department of Veterinary Public Health and Preventive Medicine, University of Maiduguri, Nigeria
Muhammed Umar
Department of Animal Reproduction, University of Agriculture, Water and Marine Sciences, Uthal, Baluchistan, Pakistan
Wessam Monther Saleh
Department of Internal and Preventive Medicine, University of Basra, Basra, Iraq

Abstract


Aim: This study was conducted to analyze the changes in blood profile of goats inoculated with Corynebacterium pseudotuberculosis and its immunogen mycolic acid (MA) extract.
Materials and Methods: A total of 12 clinically healthy crossbred Boer female goats were divided into three groups; A, B and C (4 goats each per group). Group A was inoculated with 2 ml sterile phosphate buffered saline via intradermal route as the negative control group whilst Group B was inoculated with 2 ml of MA extract (1 g/ml) intradermally and Group C was then inoculated with 2 ml (1×109) colony forming unit of active C. pseudotuberculosis intradermally. Blood sample was collected aseptically from the jugular vein periodically for complete blood count (CBC) analysis throughout the experimental period (3 months).
Result: A significant decrease (p<0.05) was observed in red blood cells, hemoglobin (Hb), packed cell volume, mean corpuscular volume and mean corpuscular Hb concentration in Groups B and C as compared to the control while WBCs, neutrophil, lymphocyte and basophil showed a significant increase (p<0.05) as compared to the control.
Conclusion: The inoculation of C. pseudotuberculosis and MA resulted in a significant change in the CBC, thereby, indicating that MA has a role in caseous lymphadenitis pathogenesis.

Keywords


Complete Blood Count, Corynebacterium pseudotuberculosis, Goat, Hematology, Mycolic Acid.