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Effect of Different Concentrations of Soybean Lecithin and Virgin Coconut Oil in Tris-Based Extender on the Quality of Chilled and Frozen-Thawed Bull Semen


Affiliations
1 Department of Dairy Production, University of Khartoum, Sudan
2 Department of Veterinary Clinical Studies, Universiti Putra Malaysia, UPM, Serdang-43400, Selangor Darul Ehsan, Malaysia
3 Department of Veterinary Pre-Clinical Sciences, Universiti Putra Malaysia, UPM, Serdang-43400, Selangor Darul Ehsan, Malaysia
 

Aim: The objective of this study was to evaluate the effects of different concentrations of soybean lecithin (SL) and virgin coconut oil (VCO) in Tris-based extender on chilled and frozen-thawed bull semen quality parameters.
Materials and Methods: A total of 24 ejaculates were collected from four bulls via an electroejaculator. Semen samples were diluted with 2% VCO in Tris-based extender which consists of various concentrations of SL (1, 1.25, 1.5, and 1.75%). A 20% egg yolk in Tris used as a positive control (C+). The diluted semen samples were divided into two fractions; one for chilling which were stored at 4°C for 24, 72, and 144 h before evaluated for semen quality parameters. The second fraction used for freezing was chilled for 3 h at 4°C, packed into 0.25 mL straws and then cryopreserved in liquid nitrogen. The samples were then evaluated after 7 and 14 days. Chilled and frozen semen samples were thawed at 37°C and assessed for general motility using computer-assisted semen analysis, viability, acrosome integrity and morphology (eosin-nigrosin stain), membrane integrity, and lipid peroxidation using thiobarbituric acid reaction test.
Results: The results showed that all the quality parameters assessed were significantly (p<0.05) improved at 1.5% SL concentration in chilled semen. Treatment groups of 1, 1.25, 1.5, and 1.75% SL were higher in quality parameters than the control group (C+) in chilled semen. However, all the quality parameters in frozen-thawed semen were significantly higher in the C+ than the treated groups.
Conclusion: In conclusion, supplementation of 1.5% SL in 2% VCO Tris-based extender enhanced the chilled bull semen. However, there was no marked improvement in the frozen-thawed quality parameters after treatment.

Keywords

Bull Semen, Cryopreservation, Quality Parameters, Soybean Lecithin, Virgin Coconut Oil.
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  • Effect of Different Concentrations of Soybean Lecithin and Virgin Coconut Oil in Tris-Based Extender on the Quality of Chilled and Frozen-Thawed Bull Semen

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Authors

A. A. Tarig
Department of Dairy Production, University of Khartoum, Sudan
H. Wahid
Department of Veterinary Clinical Studies, Universiti Putra Malaysia, UPM, Serdang-43400, Selangor Darul Ehsan, Malaysia
Y. Rosnina
Department of Veterinary Clinical Studies, Universiti Putra Malaysia, UPM, Serdang-43400, Selangor Darul Ehsan, Malaysia
N. Yimer
Department of Veterinary Clinical Studies, Universiti Putra Malaysia, UPM, Serdang-43400, Selangor Darul Ehsan, Malaysia
Y. M. Goh
Department of Veterinary Pre-Clinical Sciences, Universiti Putra Malaysia, UPM, Serdang-43400, Selangor Darul Ehsan, Malaysia
F. H. Baiee
Department of Veterinary Clinical Studies, Universiti Putra Malaysia, UPM, Serdang-43400, Selangor Darul Ehsan, Malaysia
A. M. Khumran
Department of Veterinary Clinical Studies, Universiti Putra Malaysia, UPM, Serdang-43400, Selangor Darul Ehsan, Malaysia
H. Salman
Department of Veterinary Clinical Studies, Universiti Putra Malaysia, UPM, Serdang-43400, Selangor Darul Ehsan, Malaysia
M. A. Assi
Department of Veterinary Pre-Clinical Sciences, Universiti Putra Malaysia, UPM, Serdang-43400, Selangor Darul Ehsan, Malaysia
M. Ebrahimi
Department of Veterinary Pre-Clinical Sciences, Universiti Putra Malaysia, UPM, Serdang-43400, Selangor Darul Ehsan, Malaysia

Abstract


Aim: The objective of this study was to evaluate the effects of different concentrations of soybean lecithin (SL) and virgin coconut oil (VCO) in Tris-based extender on chilled and frozen-thawed bull semen quality parameters.
Materials and Methods: A total of 24 ejaculates were collected from four bulls via an electroejaculator. Semen samples were diluted with 2% VCO in Tris-based extender which consists of various concentrations of SL (1, 1.25, 1.5, and 1.75%). A 20% egg yolk in Tris used as a positive control (C+). The diluted semen samples were divided into two fractions; one for chilling which were stored at 4°C for 24, 72, and 144 h before evaluated for semen quality parameters. The second fraction used for freezing was chilled for 3 h at 4°C, packed into 0.25 mL straws and then cryopreserved in liquid nitrogen. The samples were then evaluated after 7 and 14 days. Chilled and frozen semen samples were thawed at 37°C and assessed for general motility using computer-assisted semen analysis, viability, acrosome integrity and morphology (eosin-nigrosin stain), membrane integrity, and lipid peroxidation using thiobarbituric acid reaction test.
Results: The results showed that all the quality parameters assessed were significantly (p<0.05) improved at 1.5% SL concentration in chilled semen. Treatment groups of 1, 1.25, 1.5, and 1.75% SL were higher in quality parameters than the control group (C+) in chilled semen. However, all the quality parameters in frozen-thawed semen were significantly higher in the C+ than the treated groups.
Conclusion: In conclusion, supplementation of 1.5% SL in 2% VCO Tris-based extender enhanced the chilled bull semen. However, there was no marked improvement in the frozen-thawed quality parameters after treatment.

Keywords


Bull Semen, Cryopreservation, Quality Parameters, Soybean Lecithin, Virgin Coconut Oil.