Open Access Open Access  Restricted Access Subscription Access

An Immunological Approach of Sperm Sexing and Different Methods for Identification of X- and Y-Chromosome Bearing Sperm


Affiliations
1 Embryo Biotechnology Laboratory, Animal Biotechnology Centre, ICAR-National Dairy Research Institute, Karnal-132001, Haryana, India
 

Separation of X- and Y-chromosome bearing sperm has been practiced for selection of desired sex of offspring to increase the profit in livestock industries. At present, fluorescence-activated cell sorter is the only successful method for separation of X- and Y-chromosome bearing sperm. This technology is based on the differences in DNA content between these two types of sperm and has been commercialized for bovine sperm. However, this technology still has problems in terms of high economic cost, sperm damage, and lower pregnancy rates compared to unsorted semen. Therefore, an inexpensive, convenient, and non-invasive approach for sperm sexing would be of benefit to agricultural sector. Within this perspective, immunological sperm sexing method is one of the attractive choices to separate X- and Y-chromosome bearing sperm. This article reviews the current knowledge about immunological approaches, viz., H-Y antigen, sex-specific antigens, and differentially expressed proteins for sperm sexing. Moreover, this review also highlighted the different methods for identification of X- and Y-sperm.

Keywords

Differentially Expressed Proteins, H-Y Antigen, Sex Specific Proteins, Sperm Identification, Sperm Sexing.
User
Notifications
Font Size

Abstract Views: 214

PDF Views: 0




  • An Immunological Approach of Sperm Sexing and Different Methods for Identification of X- and Y-Chromosome Bearing Sperm

Abstract Views: 214  |  PDF Views: 0

Authors

Shiv Kumar Yadav
Embryo Biotechnology Laboratory, Animal Biotechnology Centre, ICAR-National Dairy Research Institute, Karnal-132001, Haryana, India
Dharmendra Kumar Gangwar
Embryo Biotechnology Laboratory, Animal Biotechnology Centre, ICAR-National Dairy Research Institute, Karnal-132001, Haryana, India
Jarnail Singh
Embryo Biotechnology Laboratory, Animal Biotechnology Centre, ICAR-National Dairy Research Institute, Karnal-132001, Haryana, India
Chiranjeev Kumar Tikadar
Embryo Biotechnology Laboratory, Animal Biotechnology Centre, ICAR-National Dairy Research Institute, Karnal-132001, Haryana, India
V. Vinoth Khanna
Embryo Biotechnology Laboratory, Animal Biotechnology Centre, ICAR-National Dairy Research Institute, Karnal-132001, Haryana, India
Sudha Saini
Embryo Biotechnology Laboratory, Animal Biotechnology Centre, ICAR-National Dairy Research Institute, Karnal-132001, Haryana, India
Sunny Dholpuria
Embryo Biotechnology Laboratory, Animal Biotechnology Centre, ICAR-National Dairy Research Institute, Karnal-132001, Haryana, India
Prabhat Palta
Embryo Biotechnology Laboratory, Animal Biotechnology Centre, ICAR-National Dairy Research Institute, Karnal-132001, Haryana, India
Radhey Shyam Manik
Embryo Biotechnology Laboratory, Animal Biotechnology Centre, ICAR-National Dairy Research Institute, Karnal-132001, Haryana, India
Manoj Kumar Singh
Embryo Biotechnology Laboratory, Animal Biotechnology Centre, ICAR-National Dairy Research Institute, Karnal-132001, Haryana, India
Suresh Kumar Singla
Embryo Biotechnology Laboratory, Animal Biotechnology Centre, ICAR-National Dairy Research Institute, Karnal-132001, Haryana, India

Abstract


Separation of X- and Y-chromosome bearing sperm has been practiced for selection of desired sex of offspring to increase the profit in livestock industries. At present, fluorescence-activated cell sorter is the only successful method for separation of X- and Y-chromosome bearing sperm. This technology is based on the differences in DNA content between these two types of sperm and has been commercialized for bovine sperm. However, this technology still has problems in terms of high economic cost, sperm damage, and lower pregnancy rates compared to unsorted semen. Therefore, an inexpensive, convenient, and non-invasive approach for sperm sexing would be of benefit to agricultural sector. Within this perspective, immunological sperm sexing method is one of the attractive choices to separate X- and Y-chromosome bearing sperm. This article reviews the current knowledge about immunological approaches, viz., H-Y antigen, sex-specific antigens, and differentially expressed proteins for sperm sexing. Moreover, this review also highlighted the different methods for identification of X- and Y-sperm.

Keywords


Differentially Expressed Proteins, H-Y Antigen, Sex Specific Proteins, Sperm Identification, Sperm Sexing.