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The Effects of Quercetin on MicroRNA and Inflammatory Gene Expression in Lipopolysaccharide-Stimulated Bovine Neutrophils


Affiliations
1 Department of Veterinary Biosciences and Public Health, Chiang Mai University, Chiang Mai-50100, Thailand
2 Dairy Cow Hospital, Chiang Mai University, Mae On, Chiang Mai-50130, Thailand
3 Department of Food Animal Clinic, Chiang Mai University, Chiang Mai-50100, Thailand
 

Aim: To investigate gene expression of microRNA (miRNA) milieus (MIRLET7E, MIR17, MIR24-2, MIR146A, and MIR181C), inflammatory cytokine genes (interleukin 1β [IL1B], IL6, CXCL8, and tumor necrosis factor [TNF]), and the pathogen receptor toll-like receptor (TLR4) in bovine neutrophils under quercetin supplementation.
Materials and Methods: Isolated bovine neutrophils were incubated with bacterial lipopolysaccharide under quercetin treatment or left untreated. Real-time polymerase chain reaction was performed to determine the expression of the miRNAs and messenger RNA (mRNA) transcripts in neutrophils.
Results: Quercetin-treated neutrophils exhibited a remarkable suppression in MIR24-2, MIR146A, and MIR181C expression. Similarly, mRNA expression of IL1B, IL6, CXCL8, TLR4, and TNF genes noticeably declined in the quercetin group. Many proinflammatory genes (IL1B, IL6, and CXCL8) and the pathogen receptor TLR4 had a negative correlation with MIR146A and MIR181C as revealed by Pearson correlation.
Conclusions: Interaction between cognate mRNAs and miRNAs under quercetin supplementation can be summarized as a positive or negative correlation. This finding may help understand the effects of quercetin either on miRNA or gene expression during inflammation, especially as a potentially applicable indicator in bovine mastitis.

Keywords

Bovine Neutrophil, Gene Expression, MicroRNA, Quercetin.
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  • The Effects of Quercetin on MicroRNA and Inflammatory Gene Expression in Lipopolysaccharide-Stimulated Bovine Neutrophils

Abstract Views: 189  |  PDF Views: 0

Authors

Phongsakorn Chuammitri
Department of Veterinary Biosciences and Public Health, Chiang Mai University, Chiang Mai-50100, Thailand
Suphakit Srikok
Department of Veterinary Biosciences and Public Health, Chiang Mai University, Chiang Mai-50100, Thailand
Duanghathai Saipinta
Dairy Cow Hospital, Chiang Mai University, Mae On, Chiang Mai-50130, Thailand
Sukolrat Boonyayatra
Department of Food Animal Clinic, Chiang Mai University, Chiang Mai-50100, Thailand

Abstract


Aim: To investigate gene expression of microRNA (miRNA) milieus (MIRLET7E, MIR17, MIR24-2, MIR146A, and MIR181C), inflammatory cytokine genes (interleukin 1β [IL1B], IL6, CXCL8, and tumor necrosis factor [TNF]), and the pathogen receptor toll-like receptor (TLR4) in bovine neutrophils under quercetin supplementation.
Materials and Methods: Isolated bovine neutrophils were incubated with bacterial lipopolysaccharide under quercetin treatment or left untreated. Real-time polymerase chain reaction was performed to determine the expression of the miRNAs and messenger RNA (mRNA) transcripts in neutrophils.
Results: Quercetin-treated neutrophils exhibited a remarkable suppression in MIR24-2, MIR146A, and MIR181C expression. Similarly, mRNA expression of IL1B, IL6, CXCL8, TLR4, and TNF genes noticeably declined in the quercetin group. Many proinflammatory genes (IL1B, IL6, and CXCL8) and the pathogen receptor TLR4 had a negative correlation with MIR146A and MIR181C as revealed by Pearson correlation.
Conclusions: Interaction between cognate mRNAs and miRNAs under quercetin supplementation can be summarized as a positive or negative correlation. This finding may help understand the effects of quercetin either on miRNA or gene expression during inflammation, especially as a potentially applicable indicator in bovine mastitis.

Keywords


Bovine Neutrophil, Gene Expression, MicroRNA, Quercetin.