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Pathotypic Characterization of Newcastle Disease Virus Isolated from Vaccinated Chicken in West Java, Indonesia


Affiliations
1 Study Program of Animal Biomedical Science, IPB Graduate School, Bogor Agricultural University, Bogor, Indonesia
2 Department of Veterinary Clinic Reproduction and Pathology, Bogor Agricultural University, Bogor, Indonesia
3 Department of Animal Diseases and Veterinary Public Health, Bogor Agricultural University, Bogor, Indonesia
 

Aim: This research was conducted to differentiate and characterize eight Newcastle disease virus (NDV) isolates collected from vaccinated chicken at commercial flocks in West Java, Indonesia, in 2011, 2014 and 2015 by pathotype specific primers.
Materials and Methods: A total of eight NDV isolates collected from clinical outbreaks among commercial vaccinated flocks in West Java, Indonesia, in 2011, 2014, and 2015 were used in this study. Reverse transcription-polymerase chain reaction was used to detect and differentiate virulence of NDV strains, using three sets of primers targeting their M and F gene. First primers were universal primers to detect NDV targeting matrix (M) gene. Other two sets of primers were specific for the fusion (F) gene cleavage site sequence of virulent and avirulent NDV strains.
Results: Our results showed that three isolates belong to NDV virulent strains, and other five isolates belong to NDV avirulent strains. The nucleotide sequence of the F protein cleavage site showed 112K/R-R-Q/R-K-R/G-F117 on NDV virulent strains and 112G-K/R-Q-G-R-L117 on NDV avirulent strain.
Conclusion: Result from the current study suggested that NDV virulent strain were circulating among vaccinated chickens in West Java, Indonesia; this might possess a risk of causing ND outbreaks and causing economic losses within the poultry industry.

Keywords

F Gene, M Gene, Molecular Pathotyping, Newcastle Disease Virus, Vaccinated Chickens, Virulence.
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  • Pathotypic Characterization of Newcastle Disease Virus Isolated from Vaccinated Chicken in West Java, Indonesia

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Authors

Dwi Desmiyeni Putri
Study Program of Animal Biomedical Science, IPB Graduate School, Bogor Agricultural University, Bogor, Indonesia
Ekowati Handharyani
Department of Veterinary Clinic Reproduction and Pathology, Bogor Agricultural University, Bogor, Indonesia
Retno Damajanti Soejoedono
Department of Animal Diseases and Veterinary Public Health, Bogor Agricultural University, Bogor, Indonesia
Agus Setiyono
Department of Veterinary Clinic Reproduction and Pathology, Bogor Agricultural University, Bogor, Indonesia
Ni Luh Putu Ika Mayasari
Department of Animal Diseases and Veterinary Public Health, Bogor Agricultural University, Bogor, Indonesia
Okti Nadia Poetri
Department of Animal Diseases and Veterinary Public Health, Bogor Agricultural University, Bogor, Indonesia

Abstract


Aim: This research was conducted to differentiate and characterize eight Newcastle disease virus (NDV) isolates collected from vaccinated chicken at commercial flocks in West Java, Indonesia, in 2011, 2014 and 2015 by pathotype specific primers.
Materials and Methods: A total of eight NDV isolates collected from clinical outbreaks among commercial vaccinated flocks in West Java, Indonesia, in 2011, 2014, and 2015 were used in this study. Reverse transcription-polymerase chain reaction was used to detect and differentiate virulence of NDV strains, using three sets of primers targeting their M and F gene. First primers were universal primers to detect NDV targeting matrix (M) gene. Other two sets of primers were specific for the fusion (F) gene cleavage site sequence of virulent and avirulent NDV strains.
Results: Our results showed that three isolates belong to NDV virulent strains, and other five isolates belong to NDV avirulent strains. The nucleotide sequence of the F protein cleavage site showed 112K/R-R-Q/R-K-R/G-F117 on NDV virulent strains and 112G-K/R-Q-G-R-L117 on NDV avirulent strain.
Conclusion: Result from the current study suggested that NDV virulent strain were circulating among vaccinated chickens in West Java, Indonesia; this might possess a risk of causing ND outbreaks and causing economic losses within the poultry industry.

Keywords


F Gene, M Gene, Molecular Pathotyping, Newcastle Disease Virus, Vaccinated Chickens, Virulence.