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Effects of Intratesticular Injection of Zinc-Based Solution in Rats in Combination With Anti-Inflammatory and Analgesic Drugs During Chemical Sterilization


Affiliations
1 Department of Veterinary Medicine, Federal Rural University of Pernambuco, Recife, Brazil
2 Department of Physiology, Federal University of Parana, Curitiba, Parana, Brazil
3 Laboratory of Ultrastructure, Aggeu Magalhaes Research Center (CPqAM), Recife, Pernambuco, Brazil
4 Department of Morphology, Federal University of Paraiba, Joao Pessoa, Paraiba, Brazil
 

Aim: Chemical sterilization is a non-surgical method of contraception based on compounds injected into the testis to induce infertility. However, these injections can cause discomfort and pain able to impair the recovery of animals after this treatment. The objective of this study was to investigate if anti-inflammatories or pain relievers inhibited the sterilizing effect of zinc gluconate-based solution on the testis.

Materials and Methods: Adult rats were treated in groups: G1 (control), G2 (dimethyl sulfoxide+dipyrone); G3 (dipyrone/ zinc); G4 (dipyrone+celecoxib/zinc); G5 (dipyrone+meloxicam/zinc), and G6 (dipyrone+dexamethasone/zinc) in a single dose per day during 7 days. Animals were analyzed at 7, 15, and 30 days after treatments.

Results: The zinc-induced a widespread testicular degeneration and decreased testosterone levels even in combination with antiinflammatories or pain relievers. Testis, epididymis, prostate, and seminal vesicle had a weight reduction. The anti-inflammatory effect of dexamethasone interfered in the desired action of zinc gluconate in the 1st 15 days and celecoxib up to 7 days.

Conclusion: Meloxicam plus dipyrone did not impair the chemical sterilization based on zinc gluconate, and it can be used to reduce nociceptive effects in animals after chemical sterilization.

Keywords

Analgesic, Anti-Inflammatory, Contraception, Testicular Degeneration, Zinc Gluconate.
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  • Effects of Intratesticular Injection of Zinc-Based Solution in Rats in Combination With Anti-Inflammatory and Analgesic Drugs During Chemical Sterilization

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Authors

Simone Regina Barros De Macedo
Department of Veterinary Medicine, Federal Rural University of Pernambuco, Recife, Brazil
Luiz Andre Rodrigues De Lima
Department of Veterinary Medicine, Federal Rural University of Pernambuco, Recife, Brazil
Sandra Maria De Torres
Department of Veterinary Medicine, Federal Rural University of Pernambuco, Recife, Brazil
Vinicius Vasconcelos Gomes De Oliveira
Department of Veterinary Medicine, Federal Rural University of Pernambuco, Recife, Brazil
Rosana Nogueira De Morais
Department of Physiology, Federal University of Parana, Curitiba, Parana, Brazil
Christina Alves Peixoto
Laboratory of Ultrastructure, Aggeu Magalhaes Research Center (CPqAM), Recife, Pernambuco, Brazil
Bruno Mendes Tenorio
Department of Morphology, Federal University of Paraiba, Joao Pessoa, Paraiba, Brazil
Valdemiro Amaro Da Silva Junior
Department of Veterinary Medicine, Federal Rural University of Pernambuco, Recife, Brazil

Abstract


Aim: Chemical sterilization is a non-surgical method of contraception based on compounds injected into the testis to induce infertility. However, these injections can cause discomfort and pain able to impair the recovery of animals after this treatment. The objective of this study was to investigate if anti-inflammatories or pain relievers inhibited the sterilizing effect of zinc gluconate-based solution on the testis.

Materials and Methods: Adult rats were treated in groups: G1 (control), G2 (dimethyl sulfoxide+dipyrone); G3 (dipyrone/ zinc); G4 (dipyrone+celecoxib/zinc); G5 (dipyrone+meloxicam/zinc), and G6 (dipyrone+dexamethasone/zinc) in a single dose per day during 7 days. Animals were analyzed at 7, 15, and 30 days after treatments.

Results: The zinc-induced a widespread testicular degeneration and decreased testosterone levels even in combination with antiinflammatories or pain relievers. Testis, epididymis, prostate, and seminal vesicle had a weight reduction. The anti-inflammatory effect of dexamethasone interfered in the desired action of zinc gluconate in the 1st 15 days and celecoxib up to 7 days.

Conclusion: Meloxicam plus dipyrone did not impair the chemical sterilization based on zinc gluconate, and it can be used to reduce nociceptive effects in animals after chemical sterilization.

Keywords


Analgesic, Anti-Inflammatory, Contraception, Testicular Degeneration, Zinc Gluconate.

References