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Darmawi,
- Anti-Inflammatory Activity of Jatropha curcas Linn. Latex in Cream Formulation on CD68 Expression in Mice Skin Wound
Authors
1 Laboratory of Pathology, Syiah Kuala University, Banda Aceh 23111, ID
2 Laboratory of Histology, Syiah Kuala University, Banda Aceh 23111, ID
3 Laboratory of Pharmacology, Syiah Kuala University, Banda Aceh 23111, ID
4 Laboratory of Parasitology, Syiah Kuala University, Banda Aceh 23111, ID
5 Laboratory of Microbiology, Syiah Kuala University, Banda Aceh 23111, ID
Source
Veterinary World, Vol 11, No 2 (2018), Pagination: 99-103Abstract
Aim: The purpose of the present study was to determine the potential of Jatropha curcas latex in the cream formulation on CD68 immune expression (macrophages) during inflammatory phase wound healing process in mice skin.
Materials and Methods: Amount of 12 two-months-old male mice were used between 30 and 40 g. To surgical procedures, wound skin incision was performed 2.0 cm in length until subcutaneous on the paravertebral of each animal. The treatment was carried under locally anesthetized with procaine cream. The mice were allotted into four groups of each, entire surface of each group wound covered by base cream control, sulfadiazine 0.1% cream, J. curcas latex cream 10% and, 15%, respectively. All experiments were performed twice a day for 3 days. The wound healing was assayed in stained histological sections in immunohistochemical of the wounds. CD68 expression was investigated under a microscope.
Results: The results showed that the cream from the 10% and 15% latex of J. curcas revealed moderate immune reaction to CD68 on wound healing.
Conclusion: We concluded that the latex cream of J. curcas possesses anti-inflammatory activity in wound healing process of mice skin.
Keywords
Anti-Inflammatory, CD68, Jatropha curcas Latex Cream, Wound Healing.Full Text
- Isolation and Identification of a Cellulolytic Enterobacter from Rumen of Aceh Cattle
Authors
1 Syiah Kuala University, Darussalam, Banda Aceh-23111, ID
2 Department of Veterinary Infectious Diseases and Veterinary Public Health, Bogor Agricultural University, Jalan Agatis IPB, Darmaga, Bogor, ID
3 Microbiology Laboratory, Syiah Kuala University, Darussalam, Banda Aceh-23111, ID
4 Parasitology Laboratory, Syiah Kuala University, Darussalam, Banda Aceh-23111, ID
Source
Veterinary World, Vol 10, No 12 (2017), Pagination: 1515-1520Abstract
Aim: The aim of this study was to isolate and identify a cellulolytic bacterium from the rumen fluid of Aceh’s cattle. Biodegradation by cellulolytic rumen bacteria can be used as a source of cellulolytic bacteria that act to degrade feed fibrous material so as to improve the quality of nutrients and digestibility of feed ingredients at a cheaper price than the use of commercial cellulase enzymes.Materials and Methods: Samples were collected from rumen fluid of Aceh’s cattle in Abattoirs (RPH) of Banda Aceh city, Indonesia, isolation, and screening of cellulolytic bacteria were done in Microbiology Laboratory, Faculty of Veterinary Medicine, Syiah Kuala University, Banda Aceh, Indonesia.
Results: The S1 isolates showed ±2.5 cm of clear zone diameter. Microscopically, this strain was found to be a Gram-negative, Bacillus. Homology and phylogenetic tree analysis of 16S rRNA showed that S1 isolate has 91% of sequence similarity with that of Enterobacter cloacae. 91% sequence homology shown in this study proved that the S1 isolate is probably either a new species or another genus of Enterobacteriaceae.
Conclusion: Current study suggests that cellulose hydrolytic bacteria isolated from rumen fluid of Aceh cattle on Bushnell Haas medium-carboxymethylcellulose agar, and some potent cellulose degrading bacteria have been identified.
Keywords
Aceh Cattle, Cellulose, Cellulolytic Bacteria, Enterobacteriaceae, Rumen.Full Text
- Cuticular Surface Damage of Ascaridia galli Adult Worms Treated with Veitchia merrillii Betel Nuts Extract in vitro
Authors
1 Laboratory of Pathology, Syiah Kuala University, Banda Aceh, Aceh, ID
2 Laboratory of Parasitology, Syiah Kuala University, Banda Aceh, Aceh, ID
3 Laboratory of Pharmacology, Syiah Kuala University, Banda Aceh, Aceh, ID
4 Laboratory of Physiology, Syiah Kuala University, Banda Aceh, Aceh, ID
5 Laboratory of Research, Syiah Kuala University, Banda Aceh, Aceh, ID
Source
Veterinary World, Vol 10, No 7 (2017), Pagination: 732-737Abstract
Aim: The objective of this research was to in vitro evaluate the cuticular surface damage of Ascaridia galli adult worms treated with ethanolic extract of betel nuts Veitchia merrillii.
Materials and Methods: Phytochemical screening was done using FeCl3, Wagner and Dragendorff reagents, NaOH, MgHCl, and Liebermann–Burchard reaction test. Amount of 16 worms were segregated into four groups with three replicates. Four worms of each group submerged into phosphate buffered saline, 25 mg/ml, and 75 mg/ml crude ethanolic extract of V. merrillii, and 15 mg/ml albendazole. The effect of these extract was observed 40 h after incubation as soon as worms death. The worms were sectioned transversally and were explored for any cuticular histopathological changes in their body surface under microscope.
Results: We found that the ethanolic extract of V. merrillii betel nuts contains tannins, alkaloids, flavonoids, triterpenoids, and saponins. The ethanolic extract of betel nuts V. merrillii induces surface alterations caused cuticular damage of A. galli adult worms.
Conclusion: We concluded that ethanolic extract of betel nuts V. merrillii possess anthelmintic activity caused cuticular damage of A. galli adult worms.
Keywords
Anthelmintics, Ascaridia galli, Cuticle, Veitchia merrillii.Full Text