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Prastowo, Joko
- In vitro Potential Anthelmintic Activity of Biophytum petersianum on Haemonchus contortus
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Materials and Methods: Adult worm collected from naturally infected abomasums were obtained from slaughtered goats on the day of slaughter. BAE was prepared in six different concentrations (10, 20, 40, 60, 80, and 100 mg/ml) which were tested for their efficacies on ten actively moving worms. Ivermectin (1 mg/ml) was included as a reference drug, while saline water was included as a control. The dead worms from anthelmintic test then went through sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and scanning electron microscopy (SEM).
Results: Highest mean mortality in treatments group both at 2 h and 4 h observations was BAE 10%. The SDS-PAGE analysis revealed the presence of five protein bands with molecular weights 9.3, 17.1, 50.0, 63.2, and 72.7 kDa based on BAE 10%. The SEM changes observed in the in vitro trials revealed the occurrence of interactions between the BAE and the cuticle.
Conclusion: The SEM and SDS-PAGE analysis revealed ultrastructural structural changes and the decrease numbers of polypeptides on treated worms when compared to the control worms. It can thus be concluded that the BAE exhibits good anthelmintic activity against H. contortus adult worm.
Authors
Affiliations
1 Department of Parasitology, Universitas Gadjah Mada, Bulaksmur, Caturtunggal, Depok, Sleman, Daerah Istimewa Yogyakarta-55281, ID
2 Department of Parasitology, Universitas Gadjah Mada, Bulaksumur, Caturtunggal, Depok, Sleman, Daerah Istimewa Yogyakarta-55281, ID
3 Department of Pathology, Universitas Gadjah Mada, Bulaksumur, Caturtunggal, Depok, Sleman, Daerah Istimewa, Yogyakarta-55281, ID
4 Department of Internal Medicine, Universitas Gadjah Mada, Bulaksumur, Caturtunggal, Depok, Sleman, Daerah Istimewa, Yogyakarta-55281, ID
1 Department of Parasitology, Universitas Gadjah Mada, Bulaksmur, Caturtunggal, Depok, Sleman, Daerah Istimewa Yogyakarta-55281, ID
2 Department of Parasitology, Universitas Gadjah Mada, Bulaksumur, Caturtunggal, Depok, Sleman, Daerah Istimewa Yogyakarta-55281, ID
3 Department of Pathology, Universitas Gadjah Mada, Bulaksumur, Caturtunggal, Depok, Sleman, Daerah Istimewa, Yogyakarta-55281, ID
4 Department of Internal Medicine, Universitas Gadjah Mada, Bulaksumur, Caturtunggal, Depok, Sleman, Daerah Istimewa, Yogyakarta-55281, ID
Source
Veterinary World, Vol 11, No 1 (2018), Pagination: 1-4Abstract
Aim: Haemonchus contortus is a major problem in small ruminants in Indonesia. The frequent use of the anthelmintic drugs has given rise to drug-resistant populations which increase the need for new anthelminthic compounds, particularly from endemic plants. This study evaluated the in vitro effects of Biophytum petersianum crude aqueous extract (BAE) as an anthelmintic compound against H. contortus adult worm isolated from goats.Materials and Methods: Adult worm collected from naturally infected abomasums were obtained from slaughtered goats on the day of slaughter. BAE was prepared in six different concentrations (10, 20, 40, 60, 80, and 100 mg/ml) which were tested for their efficacies on ten actively moving worms. Ivermectin (1 mg/ml) was included as a reference drug, while saline water was included as a control. The dead worms from anthelmintic test then went through sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and scanning electron microscopy (SEM).
Results: Highest mean mortality in treatments group both at 2 h and 4 h observations was BAE 10%. The SDS-PAGE analysis revealed the presence of five protein bands with molecular weights 9.3, 17.1, 50.0, 63.2, and 72.7 kDa based on BAE 10%. The SEM changes observed in the in vitro trials revealed the occurrence of interactions between the BAE and the cuticle.
Conclusion: The SEM and SDS-PAGE analysis revealed ultrastructural structural changes and the decrease numbers of polypeptides on treated worms when compared to the control worms. It can thus be concluded that the BAE exhibits good anthelmintic activity against H. contortus adult worm.
Keywords
Biophytum petersianum, Crude Aqueous Extract, Haemonchus contortus, Scanning Electron Microscopy, Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis.Full Text
- Detection of Toxoplasma gondii in Cat’s Internal Organs by Immunohistochemistry Methods Labeled With-[Strept] Avidin-Biotin
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Material and Methods: Four domestic cats aged 1-2 years were used as sample in this research. The sample divided into two groups with two cats each. Cats in Group I were positive Toxoplasma based on serologically screening test, while cats in Group II were orally infected with 1×106 Toxoplasma oocyst. All samples then necropsied, and the organs including brain, liver, kidney, duodenum, jejunum, ileum, lungs, and spleen were collected for IHC method with LAB-SA.
Result: The result showed that Toxoplasma antigens were detected in ileum of both serologically positive domestic cat and the experimentally infected cats. Toxoplasma was also observed in kidney of serologically positive domestic cat. In the serologically positive domestic cat, necrotic lesions were found on ileum, kidney, and liver, whereas in experimentally infected cat, the lesion was only found on ileum.
Conclusion: The presence of Toxoplasma antigen is successfully detected in several organs of domestic cat using IHC method with the LAB-SA.
Authors
Muhammad Hanafiah
1,
Raden Wisnu Nurcahyo
2,
Rika Yuniar Siregar
3,
Joko Prastowo
2,
Sri Hartati
4,
Bambang Sutrisno
5,
Dwinna Aliza
6
Affiliations
1 Parasitology Laboratory, Syiah Kuala University, Banda Aceh, ID
2 Department of Parasitology, Gadjah Mada University, Yogyakarta, ID
3 Veterinary Public Health of Laboratory, Diseases Investigation Center, Wates, Yogyakarta, ID
4 Department of Clinic, Gadjah Mada University, Yogyakarta, ID
5 Department of Pathology, FKH UGM, Sekip Unit II Yogyakarta 55281, Yogyakarta, ID
6 Pathology Laboratory, Syiah Kuala University Banda Aceh, ID
1 Parasitology Laboratory, Syiah Kuala University, Banda Aceh, ID
2 Department of Parasitology, Gadjah Mada University, Yogyakarta, ID
3 Veterinary Public Health of Laboratory, Diseases Investigation Center, Wates, Yogyakarta, ID
4 Department of Clinic, Gadjah Mada University, Yogyakarta, ID
5 Department of Pathology, FKH UGM, Sekip Unit II Yogyakarta 55281, Yogyakarta, ID
6 Pathology Laboratory, Syiah Kuala University Banda Aceh, ID
Source
Veterinary World, Vol 10, No 9 (2017), Pagination: 1035-1039Abstract
Aim: The aims of the study are to detect the presence of Toxoplasma gondii antigen and to determine its distribution location in several organs of domestic cat using immunohistochemistry (IHC) method with Labeled-[Strept] Avidin-Biotin (LAB-SA).Material and Methods: Four domestic cats aged 1-2 years were used as sample in this research. The sample divided into two groups with two cats each. Cats in Group I were positive Toxoplasma based on serologically screening test, while cats in Group II were orally infected with 1×106 Toxoplasma oocyst. All samples then necropsied, and the organs including brain, liver, kidney, duodenum, jejunum, ileum, lungs, and spleen were collected for IHC method with LAB-SA.
Result: The result showed that Toxoplasma antigens were detected in ileum of both serologically positive domestic cat and the experimentally infected cats. Toxoplasma was also observed in kidney of serologically positive domestic cat. In the serologically positive domestic cat, necrotic lesions were found on ileum, kidney, and liver, whereas in experimentally infected cat, the lesion was only found on ileum.
Conclusion: The presence of Toxoplasma antigen is successfully detected in several organs of domestic cat using IHC method with the LAB-SA.
Keywords
Cat, Immunohistochemistry, Labeled-[Strept] Avidin-Biotin, Toxoplasma gondii.Full Text