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El-Sabrout, Karim
- Comparison of Milk Production and Milk Composition for an Exotic and a Local Synthetic Rabbit Lines
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Materials and Methods: The experiment was conducted on 80 does and their kits. Milk yield (MY) of each doe and milk composition (MC) were recorded at 3rd week of lactation.
Results: The results of MY showed insignificant difference between V-line and Alexandria line. While the results of MC indicated significant differences in milk protein and fat between the two lines. The protein electrophoresis was used for assaying variation in milk proteins between the two lines. The banding protein patterns showed seven protein bands for Alexandria line and six bands for V line. The results demonstrated one specific protein marker at 48 KDa (κ-casein) in Alexandria doe’s milk. Moreover, the results of individual body weight at weaning age indicated that Alexandria rabbits had significantly higher body weight compared with V-line rabbits (845.33 g, 664.05 g, respectively). Alexandria line had significantly lower mortality rate compared with V-line (1.5%, 2.7%, respectively).
Conclusion: The differences which obtained in Alexandria line milk may play an important role in the productive performance of rabbits.
Authors
Affiliations
1 Department of Poultry Production, P.O. Box 21545, University of Alexandria, Alexandria, EG
2 Department of Genetics, P.O. Box 21545, University of Alexandria, Alexandria, EG
1 Department of Poultry Production, P.O. Box 21545, University of Alexandria, Alexandria, EG
2 Department of Genetics, P.O. Box 21545, University of Alexandria, Alexandria, EG
Source
Veterinary World, Vol 10, No 5 (2017), Pagination: 526-529Abstract
Aim: Basic objective of this research was to compare two rabbit lines: V-line (exotic line), Alexandria (local synthetic line) for yield and composition of milk product and their effect on productive performance of rabbits.Materials and Methods: The experiment was conducted on 80 does and their kits. Milk yield (MY) of each doe and milk composition (MC) were recorded at 3rd week of lactation.
Results: The results of MY showed insignificant difference between V-line and Alexandria line. While the results of MC indicated significant differences in milk protein and fat between the two lines. The protein electrophoresis was used for assaying variation in milk proteins between the two lines. The banding protein patterns showed seven protein bands for Alexandria line and six bands for V line. The results demonstrated one specific protein marker at 48 KDa (κ-casein) in Alexandria doe’s milk. Moreover, the results of individual body weight at weaning age indicated that Alexandria rabbits had significantly higher body weight compared with V-line rabbits (845.33 g, 664.05 g, respectively). Alexandria line had significantly lower mortality rate compared with V-line (1.5%, 2.7%, respectively).
Conclusion: The differences which obtained in Alexandria line milk may play an important role in the productive performance of rabbits.
Keywords
Alexandria Line, Milk Yield, Proteins, Rabbit Performance, V-Line.Full Text
- Associations Between Single Nucleotide Polymorphisms in Multiple Candidate Genes and Body Weight in Rabbits
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Materials and Methods: Each line kits were grouped into high and low weight rabbits to identify DNA markers useful for association studies with high BW. DNA from blood samples of each group was extracted to amplify the six growth genes. SNP technique was used to study the associate polymorphism in the six growth genes and marketing BW (at 63 days) in the two rabbit lines. The purified polymerase chain reaction products were sequenced in those had the highest and lowest BW in each line.
Results: Alignment of sequence data from each group revealed the following SNPs: At nucleotide 23 (A-C) and nucleotide 35 (T-G) in MC4R gene (sense mutation) of Alexandria and V-line high BW. Furthermore, we detected the following SNPs variation between the two lines: A SNP (T-C) at nucleotide 27 was identified by MC4R gene (sense mutation) and another one (A-C) at nucleotide 14 was identified by GHR gene (nonsense mutation) of Alexandria line. The results of individual BW at market (63 days) indicated that Alexandria rabbits had significantly higher BW compared with V-line rabbits. MC4R polymorphism showed significant association with high BW in rabbits.
Conclusion: The results of polymorphism demonstrate the possibility to detect an association between BW in rabbits and the efficiency of the used primers to predict through the genetic specificity using the SNP of MC4R.
Authors
Affiliations
1 Department of Poultry Production, Aflaton St., El-Shatby, P.O. Box 21545, University of Alexandria, Alexandria, EG
2 Department of Genetics, Aflaton St., El-Shatby, P.O. Box 21545, University of Alexandria, Alexandria, EG
1 Department of Poultry Production, Aflaton St., El-Shatby, P.O. Box 21545, University of Alexandria, Alexandria, EG
2 Department of Genetics, Aflaton St., El-Shatby, P.O. Box 21545, University of Alexandria, Alexandria, EG
Source
Veterinary World, Vol 10, No 1 (2017), Pagination: 136-139Abstract
Aim: In this study, we examined parts of six growth genes (growth hormone [GH], melanocortin 4 receptor [MC4R], growth hormone receptor [GHR], phosphorglycerate mutase [PGAM], myostatin [MSTN], and fibroblast growth factor [FGF]) as specific primers for two rabbit lines (V-line, Alexandria) using nucleotide sequence analysis, to investigate association between detecting single nucleotide polymorphism (SNP) of these genes and body weight (BW) at market.Materials and Methods: Each line kits were grouped into high and low weight rabbits to identify DNA markers useful for association studies with high BW. DNA from blood samples of each group was extracted to amplify the six growth genes. SNP technique was used to study the associate polymorphism in the six growth genes and marketing BW (at 63 days) in the two rabbit lines. The purified polymerase chain reaction products were sequenced in those had the highest and lowest BW in each line.
Results: Alignment of sequence data from each group revealed the following SNPs: At nucleotide 23 (A-C) and nucleotide 35 (T-G) in MC4R gene (sense mutation) of Alexandria and V-line high BW. Furthermore, we detected the following SNPs variation between the two lines: A SNP (T-C) at nucleotide 27 was identified by MC4R gene (sense mutation) and another one (A-C) at nucleotide 14 was identified by GHR gene (nonsense mutation) of Alexandria line. The results of individual BW at market (63 days) indicated that Alexandria rabbits had significantly higher BW compared with V-line rabbits. MC4R polymorphism showed significant association with high BW in rabbits.
Conclusion: The results of polymorphism demonstrate the possibility to detect an association between BW in rabbits and the efficiency of the used primers to predict through the genetic specificity using the SNP of MC4R.
Keywords
Associated Genes, Body Weight, Prediction, Rabbit, Single Nucleotide Polymorphism.Full Text
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