Refine your search
Collections
Co-Authors
Journals
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z All
Abdelrahman, Khaled A.
- Milk Amyloid A as a Biomarker for Diagnosis of Subclinical Mastitis in Cattle
Abstract Views :179 |
PDF Views:1
Materials and Methods: A total of 272 quarter milk samples collected from 68 Friesian cows after clinical examination for detection of clinical mastitis were employed in this study. All quarter milk samples (272) were subjected to bacteriological examination, while SCs were assessed in samples (220). Following SCC estimation and bacteriological examination, the apparently normal quarter milk samples were categorized into 7 groups and MAA concentration was estimated in normal and subclinical mastitic milk samples.
Results: Prevalence of clinical mastitis was 19.12 % (52 quarters), while 80.88 % (220 quarters) were clinically healthy with normal milk secretion. Of those 220 clinically healthy quarter milk samples, 72 (32.73%) showed SCM as detected by SCC (SCC≥500,000 cells/ml). The most prevalent bacteria detected in this study were streptococci (48.53%), Staphylococcus aureus (29.41%), Escherichia coli (36.76%), and coagulase-negative staphylococci (11.76%). Results of MAA estimation revealed a strong correlation between MAA secretion level and SCC in agreement with the bacteriological examination. Interestingly, there was a prompt increase in MAA concentration in Group III (G III) (group of milk samples had SCC≤200,000 cells/ml and bacteriologically positive) than Group I (G I) (group of milk samples with SCC≤500,000 cells/ml and bacteriologically negative), as MAA concentration in G III was about 4 times its concentration in G I.
Conclusion: Our study provides a strong evidence for the significance of MAA measurement in milk during SCM, and MAA is more sensitive to IMI than SCC. This can be attributed to rapid and sensitive marker of inflammation. The advantage of MAA over other diagnostic markers of SCM is attributed the minute or even undetectable level of MAA in the milk of healthy animals, it is not influenced by factors other than mastitis, and could be estimated in preserved samples. Therefore, we recommend that estimation of MAA concentration in milk is a more useful diagnostic tool than SCC to detect SCM and to monitor the udder health in dairy cattle.
Authors
Hany Ahmed Hussein
1,
Khaled Abd El-Hamid Abd El-Razik
1,
Alaa Mohamed Gomaa
2,
Mohamed Karam Elbayoumy
3,
Khaled A. Abdelrahman
3,
H. I. Hosein
4
Affiliations
1 Department of Animal Reproduction and AI, Veterinary Division, National Research Centre, Dokki, Giza, EG
2 Department of Mastitis and Neonatal Diseases, Animal Reproduction Research Institute, Agriculture Research Center, Giza, EG
3 Department of Parasitology and Animal Diseases, National Research Centre, Dokki, Giza, EG
4 Department of Veterinary Medicine, Beni-Suef University, Beni Suef, EG
1 Department of Animal Reproduction and AI, Veterinary Division, National Research Centre, Dokki, Giza, EG
2 Department of Mastitis and Neonatal Diseases, Animal Reproduction Research Institute, Agriculture Research Center, Giza, EG
3 Department of Parasitology and Animal Diseases, National Research Centre, Dokki, Giza, EG
4 Department of Veterinary Medicine, Beni-Suef University, Beni Suef, EG
Source
Veterinary World, Vol 11, No 1 (2018), Pagination: 34-41Abstract
Background and Aim: Mastitis is one of the most vital noteworthy monetary risks to dairy ranchers and affects reproductive performance in dairy cattle. However, subclinical mastitis (SCM) negatively affects milk quality and quantity and associated with economic losses as clinical mastitis. It is recognizable only by additional testing. Somatic cell count (SCC) is currently used worldwide for the screening of intramammary infection (IMI) infections. However, somatic cells (SC) are affected by numerous factors and not always correlate with infection of the udder. Therefore, the aim of the present study was to evaluate the milk amyloid A (MAA) in the milk of normal and SCM cows and compare the sensitivity of both MAA secretion and SCC in response to mammary gland bacterial infection.Materials and Methods: A total of 272 quarter milk samples collected from 68 Friesian cows after clinical examination for detection of clinical mastitis were employed in this study. All quarter milk samples (272) were subjected to bacteriological examination, while SCs were assessed in samples (220). Following SCC estimation and bacteriological examination, the apparently normal quarter milk samples were categorized into 7 groups and MAA concentration was estimated in normal and subclinical mastitic milk samples.
Results: Prevalence of clinical mastitis was 19.12 % (52 quarters), while 80.88 % (220 quarters) were clinically healthy with normal milk secretion. Of those 220 clinically healthy quarter milk samples, 72 (32.73%) showed SCM as detected by SCC (SCC≥500,000 cells/ml). The most prevalent bacteria detected in this study were streptococci (48.53%), Staphylococcus aureus (29.41%), Escherichia coli (36.76%), and coagulase-negative staphylococci (11.76%). Results of MAA estimation revealed a strong correlation between MAA secretion level and SCC in agreement with the bacteriological examination. Interestingly, there was a prompt increase in MAA concentration in Group III (G III) (group of milk samples had SCC≤200,000 cells/ml and bacteriologically positive) than Group I (G I) (group of milk samples with SCC≤500,000 cells/ml and bacteriologically negative), as MAA concentration in G III was about 4 times its concentration in G I.
Conclusion: Our study provides a strong evidence for the significance of MAA measurement in milk during SCM, and MAA is more sensitive to IMI than SCC. This can be attributed to rapid and sensitive marker of inflammation. The advantage of MAA over other diagnostic markers of SCM is attributed the minute or even undetectable level of MAA in the milk of healthy animals, it is not influenced by factors other than mastitis, and could be estimated in preserved samples. Therefore, we recommend that estimation of MAA concentration in milk is a more useful diagnostic tool than SCC to detect SCM and to monitor the udder health in dairy cattle.
Keywords
Biomarkers, Milk Amyloid A, Somatic Cell Count, Subclinical Mastitis.Full Text
- Evaluation of Crude Larval Protein and Recombinant Somatic Protein 26/23 (rHcp26/23) Immunization against Haemonchus contortus in Sheep
Abstract Views :161 |
PDF Views:0
Authors
Omnia M. Kandil
1,
Khaled A. Abdelrahman
1,
Hatem A. Shalaby
1,
Seham H. M. Hendawy
1,
Nadia M. T. Abu El Ezz
1,
Somia A. Nassar
1,
James E. Miller
2
Affiliations
1 Department of Parasitology and Animal Diseases, National Research Centre, Dokki, P.O. Box 12622, Giza, EG
2 Department of Pathobiological Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803, US
1 Department of Parasitology and Animal Diseases, National Research Centre, Dokki, P.O. Box 12622, Giza, EG
2 Department of Pathobiological Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803, US
Source
Veterinary World, Vol 10, No 7 (2017), Pagination: 758-763Abstract
Aim: The aim of this study was to evaluate the potential possibility of crude larval and recombinant (rHcp26/23) antigens of Haemonchus contortus for immunization to control sheep hemonchosis.
Materials and Methods: A total of 21 lambs were divided into five groups. Lambs were immunized with larval and recombinant (rHcp26/23) proteins at day 0 and day 14 and after that challenged with 5000 infective larvae of H. contortus on day 42. An unvaccinated positive control group was challenged with L3 in the meantime. An unvaccinated negative control group was not challenged.
Results: Fecal egg count reduction taking after challenge for rHcp26/23 and larval antigens was 92.2% and 38.2%, respectively, compared with the positive control group. Vaccine incited protection in rHcp26/23 and larval immunization was reflected in significant (p<0.05) decreases in worm burden; 59.9% and 40.1%, respectively.
Conclusion: Recombinant rHcp26/23 vaccine induced a partial immune response and had immune-protective effect against sheep hemonchosis.
Keywords
Antigen, Haemonchus contortus, Immunization, Larval, rHcp26/23, Sheep.Full Text