Refine your search
Collections
Co-Authors
Journals
Year
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z All
Kordshooli, Manoochehr Shabani
- Toxoplasma gondii Type I, Predominant Genotype Isolated from Sheep in South of Iran
Abstract Views :277 |
PDF Views:0
Materials and Methods: The tissue samples of diaphragm and heart from 125 sheep were collected from the main slaughterhouses of Jahrom district in South of Fars province, Iran, between Aprils and June 2013. The DNA were extracted and analyzed by nested-PCR using specific primers for SAG2 and GRA6 loci. RFLP was used to classify strains into one of the three major lineages of T. gondii.
Results: T. gondii Type I was predominant in this area. The data obtained from both loci demonstrated that the frequency of each genotype was 72% Type I, 2.4% Type III, 7.2% mixed Type I and II, 16.8% mixed Type I and III, 0.8% mixed Type II and III, and 0.8% mixed Type I, II and III.
Conclusions: Although the previously published data indicated that Type II is the predominant T. gondii genotype in sheep in the other parts of the world, this study showed that genotype I is the dominant genotype of T. gondii in the southern Iran; however, other genotypes were detected. High diversity of T. gondii genotypes including mix genotypes in lambs is of importance for the public health. These studies depict a new mapping of T. gondii genotypes pattern which could be very helpful in toxoplasmosis control and prevention.
Authors
Belal Armand
1,
Kavous Solhjoo
1,
Manoochehr Shabani Kordshooli
2,
Mohammad Hasan Davami
1,
Morteza Pourahmad
3,
Vahideh Orfaee
4
Affiliations
1 Department of Parasitology and Mycology, Jahrom University of Medical Sciences, Jahrom, IR
2 Department of Parasitic Disease, Zoonoses Research Center, Jahrom University of Medical Sciences, Jahrom, IR
3 Infectious Diseases and Tropical Medicine Research Center, Isfahan University of Medical Sciences, Isfahan, IR
4 Department of Biology, Islamic Azad University, Jahrom Branch, Jahrom, IR
1 Department of Parasitology and Mycology, Jahrom University of Medical Sciences, Jahrom, IR
2 Department of Parasitic Disease, Zoonoses Research Center, Jahrom University of Medical Sciences, Jahrom, IR
3 Infectious Diseases and Tropical Medicine Research Center, Isfahan University of Medical Sciences, Isfahan, IR
4 Department of Biology, Islamic Azad University, Jahrom Branch, Jahrom, IR
Source
Veterinary World, Vol 10, No 4 (2017), Pagination: 386-392Abstract
Aim: This study was performed to determine the genetic diversity of Toxoplasma gondii in sheep using nested-polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in Southern Iran.Materials and Methods: The tissue samples of diaphragm and heart from 125 sheep were collected from the main slaughterhouses of Jahrom district in South of Fars province, Iran, between Aprils and June 2013. The DNA were extracted and analyzed by nested-PCR using specific primers for SAG2 and GRA6 loci. RFLP was used to classify strains into one of the three major lineages of T. gondii.
Results: T. gondii Type I was predominant in this area. The data obtained from both loci demonstrated that the frequency of each genotype was 72% Type I, 2.4% Type III, 7.2% mixed Type I and II, 16.8% mixed Type I and III, 0.8% mixed Type II and III, and 0.8% mixed Type I, II and III.
Conclusions: Although the previously published data indicated that Type II is the predominant T. gondii genotype in sheep in the other parts of the world, this study showed that genotype I is the dominant genotype of T. gondii in the southern Iran; however, other genotypes were detected. High diversity of T. gondii genotypes including mix genotypes in lambs is of importance for the public health. These studies depict a new mapping of T. gondii genotypes pattern which could be very helpful in toxoplasmosis control and prevention.
Keywords
Genotyping, GRA6 Gene, Iran, Polymerase Chain Reaction-Restriction Fragment Length Polymorphism, SAG2 Gene, Sheep, Toxoplasma gondii.- A Reducing Trend of Fasciolosis in Slaughtered Animals Based on Abattoir Data in South of Iran
Abstract Views :249 |
PDF Views:0
Materials and Methods: The daily records for cattle, sheep, and goats slaughtered in the abattoir were extracted from the archived documents of the recent 5 years (2011-2015) and used as the source of data. The collected data were statistically analyzed for finding any probable correlation between the various factors associated with fasciolosis.
Results: Our results showed that 3.44% of all slaughtered animals during 2011-2015 were infected with Fasciola spp. The mean prevalence of fasciolosis for cattle, sheep, and goat was 11.15%, 5.22%, and 2.15%, respectively. In addition, the highest infection rate was in winter (4.02%), and the lowest were entered in summer (2.86%).
Conclusion: Our findings showed a reducing trend during the 5 years. Improving the animal husbandry and increasing the awareness through fasciolosis may be a logical explanation for this trend. Since there have been suggested numerous factors associated with the epidemiology of fasciolosis, further studies seem essential for better clarifying the various aspects of fasciolosis in areas.
Authors
Manoochehr Shabani Kordshooli
1,
Kavous Solhjoo
1,
Belal Armand
2,
Hamidreza Dowlatkhah
3,
Masoud Esmi Jahromi
4
Affiliations
1 Department of Parasitic Disease, Zoonoses Research Center, Jahrom University of Medical Sciences, Jahrom, IR
2 Department of Parasitology and Mycology, Jahrom University of Medical Sciences, Jahrom, IR
3 Department of Parasitic Disease, Research Center for Non-Communicable Diseases, Jahrom University of Medical Sciences, Jahrom, IR
4 Jahrom City Veterinary Offices, Jahrom, IR
1 Department of Parasitic Disease, Zoonoses Research Center, Jahrom University of Medical Sciences, Jahrom, IR
2 Department of Parasitology and Mycology, Jahrom University of Medical Sciences, Jahrom, IR
3 Department of Parasitic Disease, Research Center for Non-Communicable Diseases, Jahrom University of Medical Sciences, Jahrom, IR
4 Jahrom City Veterinary Offices, Jahrom, IR
Source
Veterinary World, Vol 10, No 4 (2017), Pagination: 418-423Abstract
Aim: Fascioliasis is a zoonosis infection caused by the liver trematodes (Fasciola spp.) which have been considered to be an important disease in livestock. After several large outbreaks, fascioliasis remains one of the serious health concerns of Iran. This study was conducted to evaluate the prevalence and possible trends of fascioliasis in slaughtered animals in South of Iran based on abattoir data during a period of 5 years.Materials and Methods: The daily records for cattle, sheep, and goats slaughtered in the abattoir were extracted from the archived documents of the recent 5 years (2011-2015) and used as the source of data. The collected data were statistically analyzed for finding any probable correlation between the various factors associated with fasciolosis.
Results: Our results showed that 3.44% of all slaughtered animals during 2011-2015 were infected with Fasciola spp. The mean prevalence of fasciolosis for cattle, sheep, and goat was 11.15%, 5.22%, and 2.15%, respectively. In addition, the highest infection rate was in winter (4.02%), and the lowest were entered in summer (2.86%).
Conclusion: Our findings showed a reducing trend during the 5 years. Improving the animal husbandry and increasing the awareness through fasciolosis may be a logical explanation for this trend. Since there have been suggested numerous factors associated with the epidemiology of fasciolosis, further studies seem essential for better clarifying the various aspects of fasciolosis in areas.