Veterinary World

Dwi Desmiyeni Putri ¹, Ekowati Handharyani ², Retno Damajanti Soejoedono ³, Agus Setiyono ², Ni Luh Putu Ika Mayasari ³, Okti Nadia Poetri ³

Affiliations
1 Study Program of Animal Biomedical Science, IPB Graduate School, Bogor Agricultural University, Bogor, ID
2 Department of Veterinary Clinic Reproduction and Pathology, Bogor Agricultural University, Bogor, ID
3 Department of Animal Diseases and Veterinary Public Health, Bogor Agricultural University, Bogor, ID

Abstract

Aim: This research was conducted to differentiate and characterize eight Newcastle disease virus (NDV) isolates collected from vaccinated chicken at commercial flocks in West Java, Indonesia, in 2011, 2014 and 2015 by pathotype specific primers.
Materials and Methods: A total of eight NDV isolates collected from clinical outbreaks among commercial vaccinated flocks in West Java, Indonesia, in 2011, 2014, and 2015 were used in this study. Reverse transcription-polymerase chain reaction was used to detect and differentiate virulence of NDV strains, using three sets of primers targeting their M and F gene. First primers were universal primers to detect NDV targeting matrix (M) gene. Other two sets of primers were specific for the fusion (F) gene cleavage site sequence of virulent and avirulent NDV strains.
Results: Our results showed that three isolates belong to NDV virulent strains, and other five isolates belong to NDV avirulent strains. The nucleotide sequence of the F protein cleavage site showed ¹¹²K/R-R-Q/R-K-R/G-F¹¹⁷ on NDV virulent strains and ¹¹²G-K/R-Q-G-R-L¹¹⁷ on NDV avirulent strain.
Conclusion: Result from the current study suggested that NDV virulent strain were circulating among vaccinated chickens in West Java, Indonesia; this might possess a risk of causing ND outbreaks and causing economic losses within the poultry industry.

Keywords

F Gene, M Gene, Molecular Pathotyping, Newcastle Disease Virus, Vaccinated Chickens, Virulence.

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