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Ybanez, Rochelle Haidee D.
- Profile and Artificial Insemination Practices of Technicians and the Artificial Insemination Success Rates in Leyte, Samar, and Biliran, Philippines (2011-2015)
Authors
1 Department of Biology and Environmental Science, College of Science , University of the Philippines Cebu, Lahug, Cebu City 6000, PH
2 Philippine Carabao Center at Visayas State University, Visca, Baybay City 6521-A, Leyte, PH
Source
Veterinary World, Vol 10, No 2 (2017), Pagination: 181-186Abstract
Background: Artificial insemination (AI) is a reproductive biotechnology that may be influenced by several factors, including the profile of the technicians and the practices used. Assessing technician's profile and their AI practices can be significant in improving AI success rate.
Aim: This study aimed to know the profile and current practices used by AI technicians (AITs), to determine the success rates of AI in water buffaloes in Leyte, Samar, and Biliran from 2011 to 2015, and to evaluate the possible association between the parameters investigated.
Materials and Methods: A total of 50 AITs from Leyte, Samar and Biliran, Philippines, were interviewed using a fixed questionnaire about their profile and employed AI practices, and 20,455 AI-related records of the Philippine Carabao Center (PCC) at Visayas State University (VSU), Baybay City, Leyte, were screened and analyzed. AI success rates were determined by retrospective analysis of the gathered data. Statistical analysis was performed between the technician profile and practices and the AI success rates.
Results: Results revealed that most of the technicians were male, around 31-40 years old, married, college graduates, working under local government units, had other sources of income, and with 1-5 years of continuous AI practice averaging 51-100 inseminations per year. Most of them attended only one basic training seminar, which was conducted more than 3 years ago in PCC in VSU. AI success rates were recorded highest in 2011 and lowest in 2015. Statistical analyses showed that some technician profile parameters (civil status, average AI per year, and the training center) and several practices (checking of soft cervix, rectal palpation, thawing temperature method, straw cutting method, and semen deposition) might have an influence on the success of AI.
Conclusion: This study documents the first report on AIT's profile and their employed AI practices and the AI success rates in Leyte, Samar, and Biliran, Philippines. Selected profile parameters and AI practices may influence AI success rates. AITs should perform more AI services and revisit the employed practices.
Keywords
Artificial Insemination, Artificial Insemination Technician, Philippines, Profile, Water Buffalo.- Detection of Ehrlichia, Anaplasma, and Babesia spp. in Dogs in Cebu, Philippines
Authors
1 College of Science, University of the Philippines Cebu, Gorordo Avenue, Lahug, Cebu City-6000, PH
2 College of Veterinary Medicine at Barili Campus and College of Arts and Sciences at Main Campus, Cebu Technological University, Cor. M. J. Cuenco Ave. and R. Palma St. Cebu City, Cebu-6000, PH
3 College of Veterinary Medicine, Southwestern University, Villa Aznar, Urgello St., Cebu City-6000, PH
4 National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro City-080-8555, JP
Source
Veterinary World, Vol 11, No 1 (2018), Pagination: 14-19Abstract
Background: Ehrlichia, Anaplasma, and Babesia spp. are canine pathogens transmitted by the Rhipicephalus sanguineus tick which can cause varied clinical signs. These pathogens have been investigated in the Philippines, but coinfection has not been reported yet.Aim: The aim of this study was to evaluate the presence of Ehrlichia/Anaplasma and Babesia spp. in Philippine dogs.
Materials and Methods: A total of 100 dogs from seven different veterinary establishments in Cebu, Philippines, were examined for Ehrlichia/Anaplasma and Babesia spp. infection using peripheral blood smear examination and polymerase chain reaction (PCR). Inclusion criteria included a history or presence of tick infestation, anemia, and/or thrombocytopenia. Clinical signs were recorded. Statistical analyses were performed between PCR positivity and clinical signs and hematological results.
Results: A total of 10 and 18 dogs were found to be positive for Ehrlichia/Anaplasma and Babesia spp., respectively. One animal was PCR positive for both pathogens, which is the first report of coinfection in the country. The most common clinical signs observed include inappetence (89%), lethargy (80%), thrombocytopenia (85%), and anemia (74%). Analyses revealed that inappetence (p=0.044) and weight loss (p=0.028) were found statistically significant with Ehrlichia/Anaplasma infection. Basophil (p=0.001) and eosinophil counts (p=0.000) were also found significantly different between Ehrlichia/Anaplasma spp.-positive and -negative dogs. On the other hand, differential monocyte count (p=0.009) was found significantly different between Babesia spp.-positive and -negative dogs.
Conclusion: The present study showed low infection rates of canine ehrlichiosis/anaplasmosis and babesiosis and provided additional evidence for the presence of the pathogens in the area.