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Bellik, Y.
- Effect of the Essential Oil of Rosmarinus officinalis (L.) on Rooster Sperm Motility During 4°C Short-Term Storage
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Materials and Methods: R. officinalis essential oil was analyzed using gas chromatography coupled to mass spectrometry to identify the active components. 10 of 45-week-old Hubbard commercial broilers were subjected to biweekly semen collections during 3 weeks. At each collection, sperm was pooled and divided into four aliquots and then diluted with Tris extender supplemented with 870, 87, or 8.7 μg/ml of R. officinalis essential oil, identified as treatments R, R5, and R10, respectively. Tris-based extender without any supplementation was considered as a control group. Diluted sperm was then stored at 4°C in the refrigerator and analyzed at 0, 6, 24, and 48 h using a computer-assisted sperm analyzer. Different semen parameters were measured including total motility, progressive motility, gametes velocities (straight line velocity [VSL], curvilinear velocity [VCL], and average path velocity [VAP]), amplitude of the lateral head displacement [ALH], and beat-cross frequency [BCF].
Results: The phytochemical analysis of R. officinalis essential oil revealed the presence of 25 active components including seven major molecules: Camphor (18.88%), camphene (5.17%), 1,8-cineole (7.85%), β-thujene (13.66%), α-thujene (4.87%), chrysanthenone (12.05%), and β-cubenene (7.97%). The results showed a beneficial effect of R. officinalis essential oil on sperm cells motility, particularly when using the lowest concentrations, 8.7 and 87 μg/ml. Progressive motility and gametes velocities (VCL, VSL, and VAP), materializing the quality of gametes motility, showed highly statistically significant values (p<0.01) in 8.7 and 87 μg/ml treatments, especially from 6 h of storage at 4°C. Conversely, the highest concentration (870 μg/ml) showed harmful effects with a total spermicidal activity after 24 h of storage.
Conclusion: The current results revealed the positive impact of R. officinalis essential oil on rooster sperm at 4°C short-term storage probably through fighting against oxidative stress and cold shock damages.
Authors
Affiliations
1 Ecole Nationale Superieure Veterinaire, Rue Issad Abbes, Oued Smar, Algiers, DZ
2 Department of Biology, University of Bouira, DZ
3 Department of Biology, University of El Bachir el Ibrahimi, Bordj Bou Arreridj-34000, DZ
4 Department of Animal Productions, University of Liege-4000, Liege, BE
5 Associated Laboratory in Marine and Aquaculture Ecosystems, University of Bejaia, DZ
1 Ecole Nationale Superieure Veterinaire, Rue Issad Abbes, Oued Smar, Algiers, DZ
2 Department of Biology, University of Bouira, DZ
3 Department of Biology, University of El Bachir el Ibrahimi, Bordj Bou Arreridj-34000, DZ
4 Department of Animal Productions, University of Liege-4000, Liege, BE
5 Associated Laboratory in Marine and Aquaculture Ecosystems, University of Bejaia, DZ
Source
Veterinary World, Vol 11, No 5 (2018), Pagination: 590-597Abstract
Aim: This study aimed to investigate the protective effect of Rosmarinus officinalis (L.) essential oil on rooster sperm motility during 4°C short-term storage.Materials and Methods: R. officinalis essential oil was analyzed using gas chromatography coupled to mass spectrometry to identify the active components. 10 of 45-week-old Hubbard commercial broilers were subjected to biweekly semen collections during 3 weeks. At each collection, sperm was pooled and divided into four aliquots and then diluted with Tris extender supplemented with 870, 87, or 8.7 μg/ml of R. officinalis essential oil, identified as treatments R, R5, and R10, respectively. Tris-based extender without any supplementation was considered as a control group. Diluted sperm was then stored at 4°C in the refrigerator and analyzed at 0, 6, 24, and 48 h using a computer-assisted sperm analyzer. Different semen parameters were measured including total motility, progressive motility, gametes velocities (straight line velocity [VSL], curvilinear velocity [VCL], and average path velocity [VAP]), amplitude of the lateral head displacement [ALH], and beat-cross frequency [BCF].
Results: The phytochemical analysis of R. officinalis essential oil revealed the presence of 25 active components including seven major molecules: Camphor (18.88%), camphene (5.17%), 1,8-cineole (7.85%), β-thujene (13.66%), α-thujene (4.87%), chrysanthenone (12.05%), and β-cubenene (7.97%). The results showed a beneficial effect of R. officinalis essential oil on sperm cells motility, particularly when using the lowest concentrations, 8.7 and 87 μg/ml. Progressive motility and gametes velocities (VCL, VSL, and VAP), materializing the quality of gametes motility, showed highly statistically significant values (p<0.01) in 8.7 and 87 μg/ml treatments, especially from 6 h of storage at 4°C. Conversely, the highest concentration (870 μg/ml) showed harmful effects with a total spermicidal activity after 24 h of storage.
Conclusion: The current results revealed the positive impact of R. officinalis essential oil on rooster sperm at 4°C short-term storage probably through fighting against oxidative stress and cold shock damages.
Keywords
Antioxidant, Avian Semen, Liquid Storage, Rosemary Essential Oil.Full Text
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