The Asian Journal of Horticulture

Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

Influence of Different Media on Shoot Regulation, Shoot Multiplication and Callus Induction in Long Pepper (Piper longum L.)


Affiliations
1 College of Horticulture, University of Horticultural Sciences, Bagalkot (Karnataka), India
     

   Subscribe/Renew Journal


An experiment on tissue culture studies in long pepper (Piper longum L.) was carried out in the year 1999-2001, to develop suitable protocol for plant multiplication technique at Green Earth Biotechnologies Ltd., Jigani Industrial Area, Bengaluru in collaboration with UAS, GKVK, Bengaluru. The concentration of 0.1 per cent mercuric chloride (HgCl2) for 60 seconds to achieve disinfection of leaf segment explants was found effective, giving maximum survival (10.02 %) and minimum contamination (0.70 %). MS media containing BAP (1.5 mg/lit.) + 2,4-D (1.0 mg/lit.) from leaf explants gave higher (9.72 %) response for calli induction, 1.54 mg amount of callus with green colour and very good score of callus. BAP 3.0 mg/lit. was ideal for the better proliferation and regeneration of shoot (40 %).The Highest number of multiple shoots (2.00), Length of shoot (1.04 cm) and number of leaves per shoot (3.00), was produced using BAP (1.0 mg/lit.) + NAA(0.1 mg/lit.). Best results for shoot were observed with the MS media combination containing BAP at 1.5 mg/lit., Kinetin at 1.0 mg/lit. and IAA at 0.5 mg/lit., gave the maximum number of buds per calli (36.00) and number of shoots (5.20) and length of shoots (1.62 cm). The in vitro ischolar_maining was achieved with the application of NAA at 1.0 mg/l 70 per cent ischolar_maining, number of primary ischolar_mains (2.40) length of primary ischolar_main (2.00) and number of secondary ischolar_mains (2.00).

Keywords

Long Pepper, In Vitro, Micropropagation, Tissue Culture, MS Media.
Subscription Login to verify subscription
User
Notifications
Font Size


  • Ahn, Inok and Ahn, I.O. (1996). Regeneration of ginseng (Panax ginseng C.A.Meyer) through the maturation process of somatic embryos. J. Korean Soc. Hort. Sci., 37 (6): 777-780.

  • Anwar, S., Hasmha, H. and Saeed, A.S. (1999). Callus induction and regeneration in Solanum nigrum L. in-vitro. Phytomorphology, 49 (2) : 215 -220.

  • Fitchet, M. (1988). Progress with in vitro experiments in black pepper. Infor. Bull. Citrus and Subtropical fruit Res. Inst., South Africa, 196 :15-16.

  • Gautam, P.L., Raina, R., Umesh, S., Ray chaudhuri, S.P. and Singh, B.B. (1998). In : Prospects of Medicinal plants. Indian Society of Plant Genetic Resources, New Delhi, pp. 29-36.

  • George, E.F. (1993). Plant propagation by tissue culture : Exegcties Ltd. Basingstoke, England.

  • Kamboj, V.P. (2000). Herbal medicine,Curr.Sci., 78 (1) : 35-39.

  • Murashige,T. and Skoog, F. (1962). A revised medium for rapid growth and bio assays with tobacco cultures. Physiol.Plant, 15 : 437-497.

  • Philip, V.J., Joseph, D., Trigg, G.S. and Dickinson, N.M. (1992). Micropropagation of black pepper (Piper nigrum Linn.) through shoot tip cultures. Plant Cell Rep., 12 : 41-44.

  • Rajmohan, K. (1985). Standardization of tissue culture techniques in important horticulture crops. Ph.D. Thesis, Kerala Agricultural University.

  • Razadan, M.K. (1993). In : Introduction to plant tissue culture. Oxford and IBM Publishing Co. Pvt.Ltd., New Delhi, pp. 42-45.

  • Shaji, P., Banerjee, N.S. and Das, M.R. (2000). Genetic variation and micropropagation in three varieties of Piper longum L. Curr. Sci., 78 (2) : 169-173.

  • Shylesh, B.S. and Padikkala, J. (1996). Clonal propagation of. Emblica sonchifolia.Amla, Res.Bull., 16 : 89-91.

  • Street, H.E. (1977). Laboratory organisation. In: Plant cell and tissue culture (H.E. Street Ed.), Block Well Scientific Publishers, Oxford, pp. 11-30.

  • Viswanthan, T.V. (1993). In Rooting Patterns of Tropical Crops : M. Abdul Salam and P. Abdul Wahid (eds.), Tata Mc Graw – Hill Publishing Co., New Delhi, Bala and Indian Long Pepper, Pp: 318-324.

  • Wakhu, A.K. and Sharma, R.K. (1998). Somatic embryogenesis and plant regeneration in Heracleum candicans Wall. Plant Cell Rep., 17: 866-869.


Abstract Views: 245

PDF Views: 0




  • Influence of Different Media on Shoot Regulation, Shoot Multiplication and Callus Induction in Long Pepper (Piper longum L.)

Abstract Views: 245  |  PDF Views: 0

Authors

S. Malthi
College of Horticulture, University of Horticultural Sciences, Bagalkot (Karnataka), India
V. P. Singh
College of Horticulture, University of Horticultural Sciences, Bagalkot (Karnataka), India
S. S. Saraswati
College of Horticulture, University of Horticultural Sciences, Bagalkot (Karnataka), India
H. T. Sakhubai
College of Horticulture, University of Horticultural Sciences, Bagalkot (Karnataka), India
P. M. Preeti
College of Horticulture, University of Horticultural Sciences, Bagalkot (Karnataka), India

Abstract


An experiment on tissue culture studies in long pepper (Piper longum L.) was carried out in the year 1999-2001, to develop suitable protocol for plant multiplication technique at Green Earth Biotechnologies Ltd., Jigani Industrial Area, Bengaluru in collaboration with UAS, GKVK, Bengaluru. The concentration of 0.1 per cent mercuric chloride (HgCl2) for 60 seconds to achieve disinfection of leaf segment explants was found effective, giving maximum survival (10.02 %) and minimum contamination (0.70 %). MS media containing BAP (1.5 mg/lit.) + 2,4-D (1.0 mg/lit.) from leaf explants gave higher (9.72 %) response for calli induction, 1.54 mg amount of callus with green colour and very good score of callus. BAP 3.0 mg/lit. was ideal for the better proliferation and regeneration of shoot (40 %).The Highest number of multiple shoots (2.00), Length of shoot (1.04 cm) and number of leaves per shoot (3.00), was produced using BAP (1.0 mg/lit.) + NAA(0.1 mg/lit.). Best results for shoot were observed with the MS media combination containing BAP at 1.5 mg/lit., Kinetin at 1.0 mg/lit. and IAA at 0.5 mg/lit., gave the maximum number of buds per calli (36.00) and number of shoots (5.20) and length of shoots (1.62 cm). The in vitro ischolar_maining was achieved with the application of NAA at 1.0 mg/l 70 per cent ischolar_maining, number of primary ischolar_mains (2.40) length of primary ischolar_main (2.00) and number of secondary ischolar_mains (2.00).

Keywords


Long Pepper, In Vitro, Micropropagation, Tissue Culture, MS Media.

References