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Antiproliferative Effect of Alpha Amyrin on Hep2 Cells by Inducing Cytotoxicity and Oxidant Antioxidant Status Modifications


Affiliations
1 Department of Biochemistry and Biotechnology, Annamalai University, Annamalai Nagar - 608 002, Tamil Nadu, India
 

Alpha amyrin, a plant bioactive triterpenoids, present in higher plants and particularly in Pergularia daemia. The compound alpha amyrin is a potent antioxidant, anti-inflammatory and antinociceptive, antipruritic, gastro protective and hepatoprotective agent. The anticancer effect of alpha amyrin has not been examined in human laryngeal cancer model. So this study evaluated its potential on Hep2 cells and anti-oxidant modifications. The current investigations explored the alpha amyrin possessed excellent manner (10, 20, 40, 80,160 and 320) μmol/ml and the IC50 value was found to be 69.32 μmol/ml. The TBARS level increased and antioxidant levels decreased in alpha amyrin treated Hep2 cells as compare to untreated cancer Hep2 cells. On the basis of our result alpha amyrin possess excellent antiproliferative ability on Hep2 cells by inducing cytotoxicity as well as oxidant antioxidant alterations. Therefore, alpha amyrin has therapeutic potential on various types of cancer.

Keywords

Alpha Amyrin, Anti-Oxidant, Laryngeal Cancer, Pergularia Daemia, Triterpenoids.
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  • Antiproliferative Effect of Alpha Amyrin on Hep2 Cells by Inducing Cytotoxicity and Oxidant Antioxidant Status Modifications

Abstract Views: 611  |  PDF Views: 352

Authors

Sankaran Mirunalini
Department of Biochemistry and Biotechnology, Annamalai University, Annamalai Nagar - 608 002, Tamil Nadu, India
Stainsloss Isabella
Department of Biochemistry and Biotechnology, Annamalai University, Annamalai Nagar - 608 002, Tamil Nadu, India
Veluchamy Vaithiyanathan
Department of Biochemistry and Biotechnology, Annamalai University, Annamalai Nagar - 608 002, Tamil Nadu, India

Abstract


Alpha amyrin, a plant bioactive triterpenoids, present in higher plants and particularly in Pergularia daemia. The compound alpha amyrin is a potent antioxidant, anti-inflammatory and antinociceptive, antipruritic, gastro protective and hepatoprotective agent. The anticancer effect of alpha amyrin has not been examined in human laryngeal cancer model. So this study evaluated its potential on Hep2 cells and anti-oxidant modifications. The current investigations explored the alpha amyrin possessed excellent manner (10, 20, 40, 80,160 and 320) μmol/ml and the IC50 value was found to be 69.32 μmol/ml. The TBARS level increased and antioxidant levels decreased in alpha amyrin treated Hep2 cells as compare to untreated cancer Hep2 cells. On the basis of our result alpha amyrin possess excellent antiproliferative ability on Hep2 cells by inducing cytotoxicity as well as oxidant antioxidant alterations. Therefore, alpha amyrin has therapeutic potential on various types of cancer.

Keywords


Alpha Amyrin, Anti-Oxidant, Laryngeal Cancer, Pergularia Daemia, Triterpenoids.

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DOI: https://doi.org/10.15613/fijrfn%2F2016%2Fv3i2%2F139485