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In Vitro Antioxidant and Free Radical Scavenging Activities of Methanolic Extract of Ocimum sanctum Linn. Seed


Affiliations
1 Department of Foods & Nutrition, Vellalar College for Women, Erode, Tamilnadu, India
 

In order to identify the potential sources of natural polyphenols and free radical scavenging activities, the edible seeds of methanolic extracts of Ocimum sanctum seed were studied for total phenolic and flavonoid contents, scavenging of free radicals of DPPH, Nitric oxide, super oxide, hydroxyl and hydrogen per oxide. Polyphenol and flavonoid content were observed sufficient amount in ocimum sanctum seed. The DPPH inhibitory and Nitric Oxide (NO) radical scavenging activities measured in-terms of their IC50 was 63.18μg/ml and 138.88μg/ml respectively. Other radicals like superoxide, hydroxyl and hydrogen peroxide scavenging activities were measured in-terms of IC50 value of 53.73μg/ml, 50.70μg/ml and 52.56μg/ml respectively.

Keywords

DPPH , Free Radical Scavenging, Hydroxyl and Hydrogen Peroxide, Nitric Oxide (NO) Superoxide, Ocimum sanctum Seed, Polyphenols.
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  • In Vitro Antioxidant and Free Radical Scavenging Activities of Methanolic Extract of Ocimum sanctum Linn. Seed

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Authors

K. Kavitha
Department of Foods & Nutrition, Vellalar College for Women, Erode, Tamilnadu, India
S. Ponne
Department of Foods & Nutrition, Vellalar College for Women, Erode, Tamilnadu, India

Abstract


In order to identify the potential sources of natural polyphenols and free radical scavenging activities, the edible seeds of methanolic extracts of Ocimum sanctum seed were studied for total phenolic and flavonoid contents, scavenging of free radicals of DPPH, Nitric oxide, super oxide, hydroxyl and hydrogen per oxide. Polyphenol and flavonoid content were observed sufficient amount in ocimum sanctum seed. The DPPH inhibitory and Nitric Oxide (NO) radical scavenging activities measured in-terms of their IC50 was 63.18μg/ml and 138.88μg/ml respectively. Other radicals like superoxide, hydroxyl and hydrogen peroxide scavenging activities were measured in-terms of IC50 value of 53.73μg/ml, 50.70μg/ml and 52.56μg/ml respectively.

Keywords


DPPH , Free Radical Scavenging, Hydroxyl and Hydrogen Peroxide, Nitric Oxide (NO) Superoxide, Ocimum sanctum Seed, Polyphenols.

References





DOI: https://doi.org/10.15613/fijrfn%2F2015%2Fv2i1%2F80077