In order to identify the potential sources of natural polyphenols and free radical scavenging activities, the edible seeds of methanolic extracts of Ocimum sanctum seed were studied for total phenolic and flavonoid contents, scavenging of free radicals of DPPH, Nitric oxide, super oxide, hydroxyl and hydrogen per oxide. Polyphenol and flavonoid content were observed sufficient amount in ocimum sanctum seed. The DPPH inhibitory and Nitric Oxide (NO) radical scavenging activities measured in-terms of their IC50 was 63.18μg/ml and 138.88μg/ml respectively. Other radicals like superoxide, hydroxyl and hydrogen peroxide scavenging activities were measured in-terms of IC50 value of 53.73μg/ml, 50.70μg/ml and 52.56μg/ml respectively.
Keywords
DPPH , Free Radical Scavenging, Hydroxyl and Hydrogen Peroxide, Nitric Oxide (NO) Superoxide, Ocimum sanctum Seed, Polyphenols.
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