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A Study of Anti-hyperglycemic and Insilico Aldose Reductase Inhibitory Effect of Terpenoids of Euphorbia Antiquorum Linn. in Alloxan Induced Diabetic Rats
Objective: To study the anti-hyperglycemic and aldose reductase inhibitory effect of Euphorbia antiquorum Linn. in Alloxan induced diabetic rats and molecular docking studies. Materials and methods: Diabetic rats were treated with 200 mg/kg of Ethanol, Petroleum ether and Aqueous extracts of E. antiquorum Linn. The blood glucose level was estimated for 8 hrs after drug treatment. Glide algorithm is used to dock the terpenoids Antiquol A, Antiquol B, and Antiquol C against the aldose reductase in standard precision mode. Results: The obtained results showed that all the extracts of E. antiquorum Linn., has hypoglycemic effect, the ethanol extract (69%) had more hypoglycemic effect which is comparable with standard drug Glibenclamide (80%). Molecular docking study reveals that Antiquol A was found to be one of the best aldose reductase inhibitor with glide score -9.07 kcal/mol. Conclusion: Our data conclude that the plant E. antiquorum has anti-diabetic as well as aldose reductase inhibitory effect.
Keywords
Euphorbia Antiquorum Linn, Diabetes Mellitus, Aldose Reductase, Terpenoids
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- Onoagbe IO, Negbenebor EO, Ogbeide VO, Dawha IH, Attah V, Lau HU and Omonkhua AA (2010) a study of the anti-diabetic effects of urena lobata and sphenostylis stenocarpa in streptozotocin-induced diabetic rats. Euro. J.Scientific Res. 43(1),6-14
- Bhaskar Chaurasia, Ravindra Singh Dhakad, Virendra Kumar Dhakar and Jain PK (2011) Preliminary phytochemical and pharmacological (Antidiabetic) screening of Cassia tora Linn. Int. J. pharmacy & life sci. 2 (5),759-766
- Mankil Jung, Moonsoo Park, Hyun Chul Lee, Yoon-Ho Kang, Eun Seok Kang and Sang Ki Kim (2006) Antidiabetic agents from medicinal plants. Curr. Med.Chem.13,1203-1218
- Marjorie Dunlop (2000) Aldose reductase and the role of the polyol pathway in diabetic nephropathy. Kidney International.58, Suppl. 77,S-3–S-12
- Bhanuprakash Reddy G, Muthenna P, Akileshwari C, Megha Saraswat and Mark Petrash J (2011) Inhibition of aldose reductase and sorbitol accumulation by dietary rutin. Curr.Sci.101(9),1191-1197
- Shuichi Miyamoto (2002) Molecular modeling and structure-based Drug Discovery studies of Aldose Reductase Inhibitors. Chem. Bioinformatics J. 2 (3),74-85
- Halim Eshrat M, All Hussain, Kaiser Jam, and Mala Rao (2001) Preliminary studies on the hypoglycaemic effect of abroma augusta in alloxan olabellc rats. Ind. J. Clin. Biochem. 16 (1), 77-80
- Amar B Singh, Chaturvedi JP, Narender T and Arvind K Srivastava (2008) Preliminary studies on the hypoglycemic effect of peganum harmala L. seeds ethanol extract on normal and streptozotocin induced diabetic rats. Ind.J.Clin.Biochem.23(4),391-393
- De Silva1 WAPP, Manuweera GK and Karunaratne1 SHPP (2008) Insecticidal activity of Euphorbia antiquorum L. latex and its preliminary chemical analysis. J. Natn. Sci .Foundation Sri Lanka.36(1),15-23
- Jyothi TM, Prabhu K, Jayachandran E, Lakshminarasu S and Ramachandra Setty S (2007) Hepatoprotective and antioxidant activity of Euphorbia Antiquorum. Phcognosy Magazine. 4(13),133-139
- Anand Nirmal Harpalani, Ashok Dundappa Taranalli, Kishor Vasant Otari, Ravindra Veerayya Karadi and Rajkumar Virbhadrappa Shete (2011) Anti-inflammatory and anti-arthritic potential of aqueous and alcoholic extracts of Euphorbia antiquorum Linn., Pharmacologyonline. 2,287-298
- Etuk EU and Muhammed B (2010) Evidence based analysis of chemical method of induction of diabetes mellitus in experimental rats. Int. J. Res. Pharm. Sci.1(2),139-142
- Pappu Srinivasan, Arumugam Sudha, Ramar Manikandan and Chinnasamy Arulvasu (2011) Molecular docking studies of 1, 2 disubstituted idopyranose from vitex negundo with anti-diabetic activity of type 2 diabetes. Int. J. Pharma & BioSci.2(1),B 68- B 83
- Dipankar Sengupta, Deeptak Verma and Pradeep K Naik (2007) Docking mode of delvardine and its analogues into the p66 domain of HIV-1 reverse transcriptase: screening using molecular mechanics- generalized born/surface area and absorption, distribution, metabolism and excretion properties. J. Biosci.32(7), 1307-1316
- Tannistha Nandi (2008) Proposed lead molecules against Hemagglutinin of avian influenza virus (H5N1). Bioinformation. 2(6),240-244
- Kesavan Sabitha (2012) Nilotinib based pharmacophore models for BCR-ABL. Bioinformation.8(14),658-663
- Etuk EU (2010) Animals models for studying diabetes mellitus. Agri.Bio. J.North America. 1(2),130-134
- David A Cano, Ingrid C Rulifson, Patrick W Heiser, Lamorna B Swigart, Stella Pelengaris, Mike German, Gerard I Evan, Jeffrey A Bluestone and Matthias Hebrok (2008) Regulated beta cell regeneration in the adult mouse pancreas. Diabetes.57 (4), 958-966.
- Roberto De Haro- Hernandez, Lourdes Cabrera-Munoz and Jose D Mendez (2004) Regeneration of β- cells and neogenesis from small ducts (or) acinar cells promote recovery of endocrine pancreatic function in Alloxan-treated rats. Arch.Med.Res.35(2),114-120
- Abu Hasanat Md Zulfiker, Farhana Alam Ripa, Md Mahbubur Rahman, Obayed Ullah M, Kaiser Hamid Md Mahbubur Rahman Khan and Md Sohel Rana (2010) Antidiabetic and antioxidant effect of Scoparia dulcis in alloxan induced albino mice. Int. J. Pharm. Tech Res. 2(4),2527-2534,
- Muthenna P, Suryanarayana P, Shravan K Gunda, Mark Petrash J and Bhanuprakash Reddy G (2009) Inhibition of aldose reductase by dietary antioxidant curcumin: Mechanism of inhibition, specificity and significance. FEBS Letters. pp: 3637-3642
- Chen Yu, Tian Xue-jun, LI Yun-fang and Yang Guang-zhong (2009) Terpenoids from Euphorbia antiquorum L. Acta Pharmaceutica Sinica. 44(10),1118-1122
- Toshihiro Akihisa EM, Kithsiri Wijeratne, Harukuni Tokuda, Fumio Enjo and Masakazu Toriumi et al. (2002) Eupha-7,9(11),24- trien-3β-ol (“Antiquol C”) and Other Triterpenes from Euphorbia antiquorum Latex and Their Inhibitory Effects on Epstein-Barr Virus Activation. J. Natural Products. 65, 158-162
- Catherine Koukoulitsa, Fabrice Bailly, Kiriaki Pegklidou and Vassilis J (2010) Demopoulos and Philippe Cotelle (2010) Evaluation of aldose reductase inhibition and docking studies of 6’-nitro and 6’,6’’-dinitrorosmarinic acids European J. Med. Chem. 45,1663-1666
- Ivan tarle, David W Borhani, David K Wilson, Florante A Quiocho, and Mark Petrash J (1993) Probing the Active Site of Human Aldose Reductase Site - directed mutagenesis of ASP-43, TYR-48, LYS-77, AND HIS-110*. The J. Bio. Chem. 268 (34), 25687-25693
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