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Botanical, Chemical and Pharmacological Review of Withania somnifera (Indian Ginseng): an Ayurvedic Medicinal Plant
Withania somnifera (L.) Dunal is a well known and important medicinal plant widely used in several indigenous systems of medicine for the treatment of various ailments, viz. asthma, bronchitis, inflammatory diseases, ulcer and stomach problems. Steroidal lactones have been reported as the major phytoconstituents of this species. Different pharmacological experiments in a number of in vitro and in vivo models have convincingly demonstrated the ability of W. somnifera to exhibit anti-inflammatory, anti-oxidative, antimicrobial, anti-anxiety, aphrodisiac, immunomodulation, anti-diabettic, anti-ulcer, anticancer, central nervous system depressant and hepatoprotective activities, lending support to the rationale behind several of its traditional uses. The species is also used to treat some neurological disorders like Parkinson's and Alzheimer's. The molecules such as withaferinA, withanolide A and withanolide D isolated from this plant are potential bioactive molecules. Due to the remarkable biological activity of W. somnifera and its constituents, it will be appropriate to develop them as a medicine and make them more potent by chemical modifications and biotransformation. This review has covered botany, chemistry and pharmacology of the plant besides its traditional and folkloric uses.
Keywords
W. somnifera, Steroidal Lactones, Withanolides, Ayurveda, Pharmacology
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- Agarwal R, Diwanay S, Patki P, Patwardhan B (1999) Studies on immunomodulatory activity of Withania somnifera (ashwagandha) extracts in experimental immune inflammation. J Ethanopharmocol . 67, 27–35.
- Ahmad M, Saleem S, Ahmad AS, Ansari MA, Yousuf S, Hoda MN, Islam F (2005) Neuroprotective effects of Withania somnifera on 6-hydroxydopamine induced Parkinsonism in rats. Hum. Exp. Toxicol . 24, 137-147.
- Al Hindawi MK, Al Khafaji SH and Abdul-Nabi MH (1992) Anti-granuloma activity of Iraqi Withania somnifera. J. Ethnopharmacol . 37, 113-116.
- Anbalagan K and Sadique J (1984) Role of prostaglandins in acute phase proteins in inflammation. Biochem. Med. 31, 236-245.
- Andulla B and Radhika B (2000) Hypoglycemic, diuretic and hypocholesterolemic effect of winter cherry (Withania somnifera) ischolar_main. Indian J. Exp. Biol. 38, 607-609.
- Anonymous (1976) In: The Wealth of India, (Raw Materials), CSIR: New Delhi, India. 10, 580-585.
- Anonymous (2004) Monograph: Withania somnifera. Altern. Med. Rev. 9, 211-214.
- Archana R and Namasivayan A (1999) Antistressor effect of Withania somnifera. J. Ethnopharmacol .64, 91-93.
- Asthana R and Raina MK (1989) Pharmacology of Withania somnifera (L.) Dunal-a review. Ind Drugs. 26, 199-205.
- Atal CK, Gupta OP, Ranghunathan K and Dhar KL (1975) Central Council for Research in Indian Medicine and Homeopathy. New Delhi, India.
- Atta-ur-Rahman, Abbas S, Jamal AS and Choudhary MI (1993) New withanolides from Withania spp. J. Nat. Prod. 56, 1000-1006.
- Atta-ur-Rahman, Jamal AS, Choudhary MI, Asif I (1991) Two withanolides from Withania somnifera. Phytochem. 30, 3824-3825.
- Bandyopadhyay M, Jha S and Tepfer D (2007) Changes in morphological phenotypes and withanolide composition of Ri-transformed ischolar_mains of Withania somnifera. Plant cell. Rep.26, 599-609.
- Bani S, Gautam M, Sheikh FA et al., (2006) Selective Th-1 up-regulating activity of Withania somnifera aqueous extract in an experimental system using flow cytometry. J. Ethnopharmacol . 107:107-115.
- Bector NP, Puri AS, Sharma D (1968) Role of Withania somnifera (Ashwagandha) in various types of Arthropathies. Ind. J. Med. Res. 56, 1581-1583.
- Begum VH and Sadique J (1988) Long term effect of herbal drug Withania somnifera on adjuvant induced arthritis in rats. Ind. J. Exp. Biol. 26, 877-882.
- Bhatnagar M, Sisodia SS and Bhatnagar R (2005) Antiulcer and antioxidant activity of Asparagus racemosus WILLD and Withania somnifera DUNAL in Rats. Ann. NY Acad. Sci. 1056, 261-278.
- Bhattacharya A, Ghosal S and Bhattacharya SK (2001) Antioxidant effect of Withania somnifera glycowithanolides in chronic footshock stress-induced perturbations of oxidative free radical scavenging enzymes and lipid peroxidation in rat frontal cortex and striatum. J. Ethnopharmacol . 74: 1-6.
- Bhattacharya SK, Bhattacharya A, Sairam K and Ghosal S (2000) Anxiolytic-antidepressant activity of Withania somnifera glycowithanolides: an experimental study. Phytomed. 7, 463-469.
- Bhattacharya SK, Goel RK, Kaur R and Ghosal S (1987) Antistress activity of sitoindosides VII and VIII, new acylsterylglucosides from Withania somnifera. Phytother. Res. 1, 32-39.
- Bhattacharya SK and Muruganandam AV (2003) Adaptogenic activity of Withania somnifera: an experimental study using a rat model of chronic stress. Pharmacol. Biochem. Behav .75, 547-555.
- Boone K (1998) Withania-The Indian ginseng and anti-aging adaptogen. Nutr. Healing. 5, 5-7.
- Budhiraja RD and Sudhir S (1987) Review of biological activity of withanolides. J. Sci. Ind. Res. 1987, 46, 488-491.
- Davis L and Kuttan G (2000) Effect of Withania somnifera on cyclophosphamide induced urotoxicity. Cancer Lett. 148(1), 4–17.
- Davis L and Kuttan G (1998) Suppressive: Effect of cyclophosphamide-induced toxicity by Withania somnifera extract in mice. J. Ethnopharmacol. 62, 209-214.
- Devi PU, Sharada AC, Solomon FE and Kamath MS (year.?) Invivo growth inhibitory effect of Withania somnifera (Ashwagandha) on a transplantable mouse tumor, Sarcoma 180. Ind. J. Exp. Biol. 30, 169-172.
- Devi PU, Sharada AC and Solomon FE (1993) Antitumor and radiosensitizing effects of Withania somnifera (ashwagandha) on a transplantable mouse tumor, Sarcoma-180. Ind. J. Exp. Biol. 31, 607-611.
- Devi PU (1999) Withania somnifera dunal (ashwagandha): Potential plant source of a promising drug for cancer chemotherapy and radiosensitisation. Ind. J. Exp. Biol. 34 (10), 927–932.
- Dhuley JN (2000) Adaptogenic and cardioprotective action of ashwagandha in rats and frogs. J. Ethanopharmocol. 70 (1),57-63.
- Dhuley JN (1998) Effect of ashwagandha on lipid peroxidation in stress-induced animals. J Ethnopharmacol. 60, 173-178.
- Dhuley JN (2001) Nootropic-like effect of Ashawagandha (Withania somnifera L.) in mice. Phytoter Res. 15, 524-528.
- Eastwood FW, Kirson I, Lavie D and Abraham A (1980) New withanolides from a cross of a South African chemotype by chemotype II (Israel) in Withania somnifera. Phytochem. 19, 1503-1507.
- Falsey RR, Marron MT, Gunaherath GM, Shirahatti N, Mahadevan D, Gunatilaka AA and Whitesell L (2006) Actin microfilament aggregation induced by withaferin A is mediated by annexin II. Nat. Chem. Biol. 2, 33-38.
- Ghosal S, Kaur R and Srivastava RS (1988) Sitoindosides IX and X, new glycowithanolides from W. somnifea. Ind. J. Nat. Prod. 4, 12-13.
- Ghosal S, Lal J, Srivastava R et al (1989) Immunomodulatory and CNS effects of sitoindosides IX and X, two new glycowithanolides from Withania somnifera. Phytother. Res. 3, 201-206.
- Grandhi A, Mujumdar AM and Patwardhan B (1994) A comparative pharmacological investigation of Ashwagandha and Ginseng. J. Ethnopharmacol. 44, 131-135.
- Gupta SK, Mohanty I, Talwar KK, Dinda A, Joshi S, Bansal P, Saxena A and Arya1 DS: Cardioprotection from ischemia and reperfusion injury by Withania somnifera: A hemodynamic, biochemical and histopathological assessment. Mol. Cell Biochem. 260, 39-47.
- Halliwell B and Gutteridge (1989) JMC Free radicals in biology & medicine. 2nd ed. Oxford: clarendon press.
- Hunziker AT (2001) Genera Solanacearum: the genera of the Solanaceae illustrated, arranged according to a new system. Gantner Verlag , Ruggell, Liechtenstein.
- Jayaprakasam B, Strasburg GA and Nair MG (2004) Potent lipid peroxidation inhibitors from Withania somnifera fruits. Tetrahed. 60, 3109-3121.
- Jayaprakasam B, Zhang Y. Seeram NP and Nair MG (2003) Growth inhibition of human tumor cell lines by withanolides from Withania somnifera leaves. Life Sci. 2003, 74, 125-132.
- Jayaram S, Walwalkar PP and Rajadhyaksha SS (1993) Evaluation of efficacy of a preparation containing combination of Indian medicinal plants in patients of generalized weakness. Ind. Drugs 30, 498-500.
- Jesberger JA and Richardson JS (1991) Oxygen free radicals and brain dysfunction. Int. J. Neurosci. 57, 1-17.
- Kapoor LD (2001) Handbook of ayurvedic medicinal plants, CRC Press: London, UK. 337-338.
- Karnick CR (1992) Clinical observations on the effect of composite herbal drugs of Withania somnifera, Panax ginseng and Tribulus terretris on psychomotor performance in healthy volunteers. Ind. Med. 4, 1-4.
- Kaul MK, Kumar A and Sharma A (2005) Reproductive biology of Withania somnifera (L.) Dunal. Curr. Sci. 88 (9), 1375-1377.
- Khan B, Ahmad SF and Bani S et al., (2006) Augmentation and proliferation of T lymphocytes and Th-1 cytokines by Withania somnifera in stressed mice. Int. Immunopharmacol. 6, 1394-1403.
- Kirson I, Abraham A and Lavie D (1977) Chemical analysis of hybrids of W. somnifera L. (Dunal) chemotype I and III Israel by Indian I (Delhi). Israel. J. Chem. 16, 20-24.
- Kothari SK, Singh CP, Kumar YV and Singh K (2003) Morphology, yield and quality of ashwagandha (Withania somnifera (L.) Dunal) ischolar_mains and its cultivation economics as influenced by tillage depth and plant population density. J. Hort. Sci. Biotechnol. 18, 422-425.
- Kulkurni SK and Ninan I (1997) Inhibition of morphine tolerance and dependence by Withania somnifera in mice. J. Ethnopharmacol. 57, 213-217.
- Kumar A, Kaul MK, Bhan MK, Khanna PK and Suri KA (2007) Morphological and chemical variation in 25 collections of the Indian medicinal plant, Withania somnifera (L) Dunal (Solanaceae). Genet Resour. Crop Evol. 45, 655-660.
- Kumar A and Kulkarni SK (2006) Effect of BR-16A (Mentat), a polyherbal formulation on drug-induced catalepsy in mice. Ind. J. Exp. Biol. 44, 45-48.
- Kumar A, Mir BA, Sehgal D, Koul S, Dar TH, Maharaj KK, Soom NR and Qazi GN (2011) Utility of multidisciplinary approach for genome diagnostics of cultivated and wild germplasm resources of medicinal Withania somnifera, and status of new species, W. ashwagandha, in the cultivated taxon. Plant Sys. Evol. 291, 141-151.
- Kumar V, Kotamballi N, Chidambara M, Bhamid S, Sudha CG, Ravishankar GA (2005) Genetically Modified Hairy Roots of Withania somnifera Dunal: A Potent Source of Rejuvenating Principles. Rejuv Res. 8, 37-45.
- Kurup PA (1956) Antibiotic principals of the leaves of Withania somnifera. Curr. Sci. 25, 57-60.
- Kuttan G (year) Use of Withania somnifera Dunal as an adjuvant during radiation therapy. Ind. J. Exp. Biol. 34, 854-856.
- Leyon PV and Kuttan G (2004) Effect of Withania somnifera on B16F-10 melanoma induced metastasis in mice. Phytother. Res. 18, 118-122.
- Lohar DR, Chaturvedi D and Varma PN (1992) Mineral elements of a few medicinally important plants. Ind. Drugs. 29, 271-273.
- Majumdar DN (1955) Withania somnifera Dunal, Part II, Alkaloidal constituents and their chemical characterization. Ind. J. Pharmacol. 17, 158-161.
- Malhotra CL, Mehta VL, Das PK and Dhalla NS (1965) Studies on Withania-ashwagandha, Kaul. V. The effect of total alkaloids (ashwagandholine) on the central nervous system. Ind. J. Physiol .Pharmacol. 9, 127-136.
- Malhotra CL, Mehta VL, Prasad K and Das PK (1965) Studies on Withania somnifera Ashwagandha Kaul (Part V). The effect of total alkaloids on the smooth muscles. Ind. J. Physiol .Pharmacol. 9, 9-15.
- Malhotra CL, Das PK, Dhalla NS and Prasad K (year) Studies on Withania ashwagandha, Kaul. III. The effect of total alkaloids on the cardiovascular system and respiration. Ind. J. Med. Res. 49, 448-460.
- Marderosion AD (2001) The review of natural products, facts and comparisons, St. Louis, MI, USA. 630-632.
- Mathur S, Kaur P and Sharma M et al (2004) The treatment of skin carcinoma induced by UV B radiation, using 1-oxo-5beta, 6beta -epoxy-with a-2-enolide, isolated from the ischolar_mains of Withania somnifera, in a rat model. Phytomed. 11, 452-460
- Menon LG, Kuttan R and Kuttan G (1997) Effect of rasayanas in the inhabitation of lung metastasis induced by B16F-10 melanoma cells. J. Exp. Clin. Cancer Res. 16, 365-368.
- Mir BA, Koul S, Kumar A, Kaul MK, Soodan AS and Raina SN (2010) Intraspecific variation in the Internal Transcribed Spacer (ITS) Regions of rDNA in Withania somnifera (L.) Dunal. Ind. J. Biotech. 9(3), 325-328.
- Mir BA, Koul S, Kumar A, Sushant S, Kaul MK and Soodan AS (2012) Reproductive behaviour and breeding system of wild and cultivated types of Withania somnifera (L.) Dunal. J. Med. Plants Res. 6 (5), 754-762.
- Mir BA, Kumar A, Koul S, Kaul MK, Raina SN and Soodan AS (2011) Assessment and characterization of genetic diversity in Withania somnifera (L.) Dunal using RAPD and AFLP markers. Afr. J. Biotech. 10(66), 14746-14756.
- Mir BA (2010) Characterization of genetic variability in Withania somnifera (L.) Dunal using biochemical and molecular markers. Thesis 2010, Guru Nanak Dev University, India.
- Mirjalili MH, Moyano E, Bonfill E, Cusido RM and Palazón J (2009) Steroidal Lactones from Withania somnifera, an ancient plant for novel medicine. Molecule, 14, 2373-2393.
- Mishra LC, Singh BB and Dagenais S (2000) Scientific basis for the therapeutic use of Withania somnifera (Ashwagandha): A Review. Altern Med. Rev. 5(4), 334-346.
- Mohanty I, Arya DS, Dinda A, Talwar KK, Joshi S and Gupta SK (2004) Mechanisms of Cardioprotective effect of Withania somnifera in experimentally induced myocardial infarction. Basic clin. Pharmacol. Toxicol. 94 (4), 184-190.
- Nadkarni KM ( 1976) Indian materia medica, Popular Prakshan Limited: Bombay, India. 1291.
- Oh JH, Lee TJ, Kim SH, Choi YH, Lee SH, Lee JM, Kim YH, Park JW and Know TK (2008) Induction of apoptosis by withaferin A in human leukaemia U937 cells through downregulation of Akt phosphorylation. Apoptosis. 13, 1494-1504.
- Owais M, Sharad KS, Shehbaz A and Saleemuddin M (2005) Antibacterial efficacy of Withania somnifera (ashwagandha) an indigenous medicinal plant against experimental murine salmonellosis. Phytomed. 12, 229-235.
- Panda S and Kar A (1998) Changes in thyroid hormone concentrations after administration of ashwagandha ischolar_main extract to adult male mice. J. Pharmacol. 50, 1065-1068.
- Panda S and Kar A (1999) Withania somnifera and Bauhinia pupurea in the regulation of circulating thyroid hormone concentrations in female mice. J. Ethnopharmacol. 67, 233-239.
- Patwardhan B, Panse GT and Kulkarni PH (1998) Ashwagandha a review. J. Natl. Integrated Med. Assoc. 30, 7-11.
- Prakash J, Gupta SK and Dinda AK (2002) Withania somnifera ischolar_main extract prevents DMBA-induced quamous cell carcinoma of skin in Swiss albino mice. Nutr. Cancer. 42, 91-97.
- Prakash J, Gupta SK, Kochupillai V, Gupta YK and Joshi S (2001) Chemopreventive activity of Withania somnifera in experimentally induced fibrosarcoma tumors in Swiss albino mice. Phytother. Res. 15(3), 240-244.
- Rasool M and Varalakshmi P (2006) Immunomodulatory role of Withania somnifera ischolar_main powder on experimental induced inflammation: an in vivo and in vitro study. Vascul. Pharmacol. 44, 406-410.
- Schliebs R, Liebmann A, Bhattacharya SK, Kumar GS and Bigl V (1997) Systemic administration of defined extracts from Withania somnifera (Indian Ginseng) and Shilajit differentially affects cholinergic but not glutamatergic and GABAergic markers in rat brain. Neurochem. Int. 30, 181-190.
- Sehgal N, Gupta A, Khader R, Shanker V, Joshi D, Mills JT, Hamel E, Khanna P, Jain SC, Thakur SS, and Ravindranath V (2012) Withania somnifera reverses Alzheimer’s disease pathology by enhancing low-density lipoprotein receptor-related protein in liver. Proc. Nat. Acad. Sci. 109(9), 3510-3515.
- Sen J and Sharma AK (1999) Micropropagation of Withania somnifera from germinating seeds and shoot tips. Plant Cell Org. Tiss. Cult. 26, 71-73.
- Sen N, Banerjee B, Das BB, Ganguly A, Sen T, Pramanik S, Mukhopadhyay S and Majumder HK (2007) Apoptosis is induced in leishmanial cells by a novel protein kinase inhibitor withaferin A and is facilitated by apoptotic topoisomerase I-DNA complex. Cell Death Differ. 14, 358-367.
- Sharad AC, Solomon FE, Devi PU et al., (1996) Antitumor and radiosensitizing effects of withaferin A on mouse Ehrlich ascites carcinoma in vivo. Acta Oncol. 35, 5-100.
- Sharma R (2004) Agro-techniques of medicinal plants. Daya Publishing House: New Delhi, India.31-33.
- Silvia DS, Eun RH, Renaud W and Shivendra VS (2008) Withaferin A Causes FOXO3a- and Bim-Dependent Apoptosis and Inhibits Growth of Human Breast Cancer Cells In vivo. Can. Res. 68, 7661-7669.
- Singh B, Saxena AK, Chandan BK, Gupta DK, Bhutani KK and Anand KK (2001) Adaptogenic activity of a novel, withanolide- free aqueous fraction from the ischolar_mains of Withania somnifera Dun. Phytother. Res. 15 (4), 311-318.
- Singh S and Kumar S (1998) Withania somnifera: The Indian Ginseng Ashwagandha, Central Institute of Medicinal and Aromatic Plants: Lucknow, India.
- Watt GA (1972) Dictionary of the economic Products of India. Cosmo Publication, Delhi, India. 6,309.
- Williamson EM (2002) Major herbs of ayurveda, Churchill Livingstone: London, UK. 2002, 322-323.
- Yang H, Shi G and Dou QP (2007) The tumor proteasome is a primary target for the natural anticancer compound withaferin A isolated from “Indian winter cherry”. Mol. Pharmacol. 71, 426-437
- Ziauddin M, Phansalkar N, Patki P et al., (1996) Studies on the immunomodulatory effects of ashwagandha. J. Ethnopharmacol. 50, 69-76.
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