Indian Journal of Drugs and Diseases

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A Study of the invivo Effect of Microgynon and Primolut-n on Albino Rat Plasma Aspartate Amino Transferase (EC 2.6.1.1) and Alanine Amino Transferase (EC 2.6.1.2) at 37°c, Ph = 9.8


Affiliations
1 Department of Biochemistry, University of Port Harcourt, Nigeria. P.M.B 5323, Port Harcourt, Nigeria
 

Oral contraceptives namely Microgynon a combined pill (0.15mg levonorgestrel and 0.03mg ethinylestradiol) and Primolut -N a mini pill (5mg norethisterone) were analysed for their in-vivo effects on albino rat plasma and erythrocyte aspartate amino transferase (AST). The in-vivo effects of the oral contraceptives on albino rat plasma and erythrocyte AST showed that the drugs inhibited the activity of the enzymes in a concentration dependent manner. The effect of the drugs on the enzymes were also time dependent with the highest inhibition obtained at 24 hours duration while the least inhibition occurred at 2 hours duration Microgynon showed the highest inhibition (7.00 ± 0.00 vs. control 31.00 ± 0.00 U/L) (P < 0.05) followed by Primolut (16.00 ± 0.00 vs. control 27.00 ± 4.00 U/L). The erythrocyte AST activity was also inhibited. The highest inhibition values obtained were Microgynon (36.00 ± 0.00 U/L) then Primolut (41.00 ± 0.00 U/L). The least inhibition values obtained were Microgynon and Primolut (67.00 ± 0.00). The in-vivo effects of the oral contraceptives on rat plasma and erythrocyte ALT showed that the drugs activated the activity of the enzymes in a concentration dependent manner. The effect of the drugs on the enzymes were also time dependent with the highest activation obtained at 24 hours duration while the least activation occurred at 2 hours duration. Primolut showed the highest activation (18.00 ± 0.00 U/L). The erythrocyte enzymes showed higher activity than the plasma enzymes. Microgynon showed the highest activity (50.00 ± 2.00 U/L). This result indicates that liver function tests are needed for women before using these drugs.

Keywords

Microgynon, Primolut –N
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  • A Study of the invivo Effect of Microgynon and Primolut-n on Albino Rat Plasma Aspartate Amino Transferase (EC 2.6.1.1) and Alanine Amino Transferase (EC 2.6.1.2) at 37°c, Ph = 9.8

Abstract Views: 458  |  PDF Views: 337

Authors

N. F. Okoye
Department of Biochemistry, University of Port Harcourt, Nigeria. P.M.B 5323, Port Harcourt, Nigeria
A. A. Uwakwe
Department of Biochemistry, University of Port Harcourt, Nigeria. P.M.B 5323, Port Harcourt, Nigeria
D. C. Belonwu
Department of Biochemistry, University of Port Harcourt, Nigeria. P.M.B 5323, Port Harcourt, Nigeria
N. C. Nwachoko
Department of Biochemistry, University of Port Harcourt, Nigeria. P.M.B 5323, Port Harcourt, Nigeria

Abstract


Oral contraceptives namely Microgynon a combined pill (0.15mg levonorgestrel and 0.03mg ethinylestradiol) and Primolut -N a mini pill (5mg norethisterone) were analysed for their in-vivo effects on albino rat plasma and erythrocyte aspartate amino transferase (AST). The in-vivo effects of the oral contraceptives on albino rat plasma and erythrocyte AST showed that the drugs inhibited the activity of the enzymes in a concentration dependent manner. The effect of the drugs on the enzymes were also time dependent with the highest inhibition obtained at 24 hours duration while the least inhibition occurred at 2 hours duration Microgynon showed the highest inhibition (7.00 ± 0.00 vs. control 31.00 ± 0.00 U/L) (P < 0.05) followed by Primolut (16.00 ± 0.00 vs. control 27.00 ± 4.00 U/L). The erythrocyte AST activity was also inhibited. The highest inhibition values obtained were Microgynon (36.00 ± 0.00 U/L) then Primolut (41.00 ± 0.00 U/L). The least inhibition values obtained were Microgynon and Primolut (67.00 ± 0.00). The in-vivo effects of the oral contraceptives on rat plasma and erythrocyte ALT showed that the drugs activated the activity of the enzymes in a concentration dependent manner. The effect of the drugs on the enzymes were also time dependent with the highest activation obtained at 24 hours duration while the least activation occurred at 2 hours duration. Primolut showed the highest activation (18.00 ± 0.00 U/L). The erythrocyte enzymes showed higher activity than the plasma enzymes. Microgynon showed the highest activity (50.00 ± 2.00 U/L). This result indicates that liver function tests are needed for women before using these drugs.

Keywords


Microgynon, Primolut –N

References