Comparative Analysis of Antioxidant Activity and Phytochemical Potential of Cassia Absus linn., Cassia Auriculata linn. and Cassia Fistula linn

Dried seeds of Cassia auriculata Linn., C.absus Linn. and C. fistula Linn. were collected from Javaathu hills, Tiruvannamalai District, Tamil Nadu, India for the comparative analysis of phyto-chemical potential with special reference to antioxidant activity. The active compounds were extracted with ethyl acetate, hexane and methanol. The methanol was found as suitable solvent and hence used for further extraction and analysis. The preliminary phytochemical screening of C. auriculata, C. absus C. fistula has revealed for the presence of alkaloids, phenolics and flavanoids in all the species. Absence of reducing sugars in C. absus, glucosides in C. auriculata and saponins in C. fistula were also observed. The quantitative determination of phenol and flavanoids was carried out in C. auriculata, C.absus and C. fistula and found that the total phenolic content as 0.18, 0.15, 0.11 and flavonoids 0.08, 0.092, 0.087% respectively. The antioxidant potential of the seed extracts recorded as much as 50% compared to α- tocopherol as standard. The Super oxide assay for the sample shows as 3% comparable to the standard α-tocopherol (5%). The Phosphomolybdenum assay and metal chelating activity indicate that the plant extract is a potential antioxidant. The details are presented in the present study.