![Open Access](https://i-scholar.in/lib/pkp/templates/images/icons/fulltextgreen.png)
![Restricted Access](https://i-scholar.in/lib/pkp/templates/images/icons/fulltextred.png)
In vitro Shoot Proliferation for Rapid and Mass Production of Quality Planting Materials of Bambusa nutans in the Climatic Conditions of Bihar, India
Objective: Bambusa nutans is a commercially important bamboo. The current study was undertaken for mass production of B. nutans in the agroclimatic conditions of Bihar.
Methods/Statistical analysis: Axillary shoot proliferation through tissue culture techniques was employed for this study. The aforesaid technique is the only method for the rapid and mass propagation of Bambusa nutans. Bamboo germplasm grown in TNB College campus was used as the source of explant. The various sizes of explants were selected to find out the most suitable for culture in the climate of Bihar. In each treatment of hormone, experiments were designed in triplicate and standard deviations were calculated. Hormone free cultures were used as control.
Findings: Out of various PGRs used for shoot proliferation, BAP (cytokinin) was found to be the most effective. With the increase in the concentrations of BAP, there was corresponding increase in the number of microshoots. Maximum initiation was observed when BAP was used in the concentration of 3mg/l. For most of the species of bamboos, NAA has been reported effective for ischolar_maining, however, we observed IBA comparatively more efficient auxin. The present findings (bud breakage, shoot multiplication, in vitro ischolar_maining and hardening procedures) will be helpful for rapid and mass production of Bambusa nutans in Bihar. This is the first in vitro culture report of bamboo in the climatic conditions of Bihar.
Applications/Improvements: The result obtained from this study has now set a defined protocol for rapid propagation of Bambusa nutans for the state of Bihar, which can help to promote economic upliftment of rural people.
Keywords
- J Geilis, J Oprins. The strategic role in biotechnology in mass scale production of woody bamboos. In: Sustainable agriculture for food, energy and industry, N. El Bassam, R.K. Bheland B.Prochnow (Eds.),London: James and James. 1998; 165-171.
- B Patel, B Gami, N Patel, V Baria, One step pre- hardening of Bambusa balcooa Roxb. Journal of phytology, 2015,7,2-4.
- KD. Mudoi, SP Saikia, M Borthakur. Effect of nodal positions, seasonal variations, shoot clump and growth regulators on micro propagation of commercially important bamboo, Bambusa nutans Wall ex. Munro. African Journal of Biotechnology, 2014, 13(9), 1961-1972.
- Y Mishra, PK Patel, S Yadav, F Shirin, SA Ansari. A micro propagation system for cloning of Bambusa tulda Roxb. Science Direct, 2008, 115, 315-318.
- I D Arya, S Arya. In vitro shoot proliferation and Somatic Embryogenesis: Means of Rapid Bamboo multiplication. 10th world bamboo congress, Korea, 2015
- BM Kavitha, S Kiran. An efficient technique for in vitro propagation of Dendrocalamus brandisii Kurz using Nodal segment. Global Journal of Microbiology and Biotechnology, 2014, 2(1), 1-10.
- P Sharma, KP Sarma. In vitro propagation of Bambusa nutans in commercial scale in Assam, India. Journal of environmental research development. 2014, 9(2), 349-352.
- P Sharma and KP Sarma. In vitro Propagation of Bambusa pallida in commercial scale in Assam, India. Journal of environmental research development.2014, 8(4), 896-900.
- P Sharma, KP Sarma. In vitro Propagation of Bambusa balcooa in commercial scale in Assam, India. International conference on Advances in Biotechnology and Pharmaceutical sciences, Bangkok, 2011, 249-251.
- R L Banik, Islam SAMN, Hadiuzzaman S, In vitro regeneration of multiple shoots in three Bamboo species. Plant Cell Tissue and Organ Culture.1993, 3(2), 101-106.
- P Sharma, KP Sarma. In vitro propagation of Bambusa tulda: An important plant for better environment. Journal of environmental research and development.2013; 7(3), 1216-1221.
- RS Singh, S Dalal, R Singh, AK Dhawan, RK Kalia, Factors influencing in vitro bud break, shoot multiplication and plantlet regeneration of Dendrocalamus hamiltonii Arn. ex Munro. Indian Journal of Plant Physiology.2012, 17(1), 9-21.
- J Brar, A Shafi, P Sood, M Anand, A Sood. In-vitro propagation, biochemical studies and assessment of clonal fidelity through molecular markers in Bambusa balcooa, Journal of tropical forest science, 2014, 26(1), 115-124
- A Sood, PS Ahuja, OP Sharma, S Godbole. In vitro protocols and field performance of elites of an important bamboo Dendrocalamus hamiltonii Nees et Arn. Ex. Munro. Plant Cell Tiss Organ. Culture. 2002; 71, 55-63.
- VM Jimenez, J Castillo, E Tavares, E Guevara, M Montie. In vitro propagation of the neotropical giant bamboo, Guadua angustifolia Kunth, through axillary shoot proliferation. Plant Cell Tissue Organ Culture.2006, 86 (3), 389-395.
- D Negi, S Saxena. Micropropagation of Bambusa balcooa Roxb. through axillary shoot proliferation, In vitro Cellular & Development Biology-Plant. 2011, 47 (5), 604-610.
- ID Arya, B Kaur, S Arya. Rapid and mass propagation of economically important Bamboo Dendrocalamus hamiltonii. Indian Journal of Energy., 2012, 1(1), 11-15
- Islam SAMN, MM Rahman. Micro-cloning in commercially important six bamboo species for mass propagation and at large scale cultivation. Plant Tissue Culture Biotechnology. 2005, 15,104-110.
- KD Mudoi, SP Saikia, A Goswami, A Gogoi, D Bora, M Borthakur. Micro propagation of Important Bamboos: A review. African Journal of Biotechnology, 2013, 12(20), 2774-2780.
- M Kaur, S Kapruwan, M Bakshi. Rapid in vitro propagation of the solid bamboo, Dendrocalamus strictus Nees, through axillary shoot proliferation. Biotechnology International, 2014, 7(3), 59-61.
- CS Lin, Chang WC. Micro propagation of Bambusa edulis though nodal explants of field grown culms and flowering of regenerated plantlets. Plant cell reports, 1998, 17(1), 616-620.
- RK Agnihotri, Nandi S. In vitro shoot cut: A high frequency multiplication and ischolar_maining method in the bamboo Dendrocalamus hamiltonii, Biotech, 2009, 8(2), 259-263.
- R Yasodha, P Sumathi, Mallinga, K Gurumurthi. Genetic enhancement and mass production of quality propagules of Bambusa nutans and Dendrocalamus membranaceous, Indian Forester, 1997, 123(4), 303-306.
- C Lin, C Liang, H Hsaio, M Lin, W Chang, In vitro flowering of green and albino Dendrocalamus latiflorus, New Forests.2007, 34, 177-186.
- S Arya, S Sharma, R Kaur, ID Arya. Direct regeneration of shoots from immature inflorescences in Dendrocalamus asper (edible bamboo) leading to mass propagation. The Journal of the American Bamboo Society, 2008, 21(1), 879-882.
- A Ndiaye, M Diallo, D Niang, Gassama - dia Y. In vitro regeneration of adult trees of Bambusa vulgaris, African Journal of Biotechnology.2006,5,1245-1248,
- J Aitken – Christie, T Kozai, M Lila Smith. Automation and environmental control in plant tissue organ culture. Springer Science+Buisness Media Dordrecht.1995, 493-516.
![](https://i-scholar.in/public/site/images/abstractview.png)
Abstract Views: 812
![](https://i-scholar.in/public/site/images/pdfview.png)
PDF Views: 473