Indian Journal of Forensic Medicine & Toxicology

Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

Acute and Subacute Toxicity of Aqueous Extract of Leaves Mixture of Aloe Buettneri (Liliaceae), Dicliptera Verticillata (Acanthaeae), Hibiscus Macranthus (Malvaceae) and Justicia Insularis (Acanthaceae) on Swiss Mice and Albinos Wistar Female Rats


Affiliations
1 Central Institute of Medicinal and Aromatic Plants, P.O.CIMAP Near Kukrail, Pinic Spot Road, Lucknow, Uttar Pradesh, India
2 Department of Biochemistry, University of Dschang; Faculty of Science, P.O. Box 67 Dschang,
3 Head of the Laboratory of Medicinal Plants, Food science and Nutrition, University of Dschang
     

   Subscribe/Renew Journal


Aloe buettneri (Liliaceae), Justicia insularis (Acanthaceae), Hibiscus macranthus (Malvaceae), Dicliptera verticillata (Acanthaceae) (ADHJ) are medicinal plants generally found in tropical and subtropical areas. The leaf mixture of these plants is used in the Western Region of Cameroon to increase the fertility, to regularize the menstrual cycle and to treat dysmenorrhoea or cases of infertility in women. In order to evaluate the toxicity of the mixture extract of these plants, the values of their LD50 and LD100 were determined in Swiss mice and the subacute toxicity studied in albinos Wistar female rats. The herbal drug induces changes in the physiological and biochemical parameters. These include changes in body and vital organ weights, in serum, hepatic, ovarian and uterine proteins and in AST, ALT and creatinine levels. The LD50 and LD100 in male mice were 27 and 32 g/kg respectively. The behaviour of the mice treated with the doses varying between 8 to 32 g/kg was significantly changed. The leaf mixture of the plants has significantly increased the reproductive organ weights of treated female rats. The serum level, uterine and ovarian proteins as well as the creatinine level increased significantly while the hepatic protein level decreased. The rate of A.S.T remained unchanged while the rate of A.L.T increased when animals were treated at the rate of 100 mg/kg. These results suggests on one hand that aqueous extract is not short-term poisonous but presents unfavourable effects in the long run (60 days) and on the other hand that the aqueous extract have a direct action on the reproductive organs and cause disturbances on cellular metabolism.

Keywords

Toxicity, Mice, Female rats, Biochemical Parameters
Subscription Login to verify subscription
User
Notifications
Font Size


  • Abayomi, S. (1984): Medicinal plants and Traditional Medecine in Africa. John Wiley and Sons Ltd, 247p.

  • Bep O. et Bever (1986): Medicinal plants in tropical West Africa. Cambridge University Press, New York. 375p.

  • Bradford, M. (1976): A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dyebinding, Anal Biochem. 72: 248-254.

  • Butenandt, A. and Jacobi, H. (1933): Female sexual hormone preparation from a plant, tokokinin and its identification with the á-follicular hormone. Zeitschrift für physiologische Chemie, 218; 204-222.

  • Burkill, H.M. (1985): The useful plants of west tropical Africa.2nd Ed. Vol.1, Royal Botanic Garden K.E.W. pp 120-145.

  • Chopra, U.N. (1933): Indigenous drugs of India. Their medical and economic aspects. The Art Press, Calcutta, India, 550p.

  • Delongeas, J. L. ; Burnel, D. ; Netter, P. ; Grignon, M. ; Mor, J. ; Royer, R.J. (1983): Toxicité et pharmacocinétique de l’oxychlorure de zincinium chez la souris et chez le rat. Journal of pharmacology, 14 (4): 437-447.

  • Emerson, S.P.; sharada, A.c.; Devi, U.P. (1993): Toxic effects of crude nut extract of plumbago nosea (Raktachitraka) on mice and rats. Journal of Ethnopharmacology, 38: pp 79-84.

  • Gornal, A.G.; Bardwil, G.; David, M.M. (1949): Determination of serum proteins by mean of Biuret reactions. Biochemistry, (177): 751-756.

  • Gatsing, D.; Reseline, A.; Jules, R.J.; Garray, I.H.; Jaryum. , K.H.; Nestor, T.; TChouanguep, F.M. and Godwin, A. (2005): Toxicological evaluation of the aqueous extract of Allium sativum bulbs on laboratory mice and rats. Cameroon Journal of Experimental Biology.Vol 01/N°1: 39-45p.

  • Guemo, T.C. (2002). Effets des extraits aqueux de Dicliptera verticillata GJH Amshoff, (Acanthacées), Hibiscus macranthus Hochst ex A. Rich (Malvacées) Justicia insularis T. Anders (Acanthacées), Aloe buettneri A. Berger (Liliacées) et de leur mélange sur le muscle utérin de la ratte en oestrus. Mémoire de maîtrise. Département de Biochimie. Université de Dschang.

  • James, T.P. and Kathleen, D. (1992): Mosby’s diagnostic and laboratory test reference. Most by year book, St. Louis, USA. 843p.

  • Jensen, E.V. and Desombre, E.R. (1972): Mechanism of action of the female sex hormones. Annual Review of Biochjemistry. 41, pp 203-230.

  • Kakeno, J.J.; Harvey, J.W.; Bruss, M.L. (1997): Clinical Biochemistry of Domestic Animals, 5th éd. Academic Press, San Diego, p 932.

  • Katzenellenbogen, B.S., Bhakoo, H.S., Ferguson, E.R., Lan; Tatee, J.; Tsia, T.L.S. and Katzenellenbogen, J.A. (1979): Estrogens and antiestrogens action in reproductive tissues and tumours. Recent Progress in Hormone Research.35, pp 259-292.

  • Lehninger, L.A. (1982): Principles of Biochemistry. New York, Worth Publishers. Inc. 1011p.

  • Palmeiro, N.M.S.; Almeida, C.E.; Ghedini, P.C.; Goulart, L.S.; Perreira, M.C.F.; Huber, S.; Da silva J.E.P.; Lopes, S. (2003): Oral toxicity of aqueous crude extract of plantago australis leaves. Journal of Ethnopharmacology.88p: 15-18.

  • Poné, K.B. (2004): Effet de l’extrait aqueux de Solanum torvum (Solanacées) sur la reproduction du rat mâle adulte. Mémoire de Maîtrise biochimie, Université de Dschang. 33p.

  • Schorderet, M. (1992) : Pharmacologie des concepts fondamentaux aux applications thérapeutiques. Eds Frisson Roche et latkine. Paris Grenoble. 920p.

  • Telefo, P.B. (1998): Contribution à l’étude des plantes médecinales du Cameroun : Influence de l’extrait aqueux du mélange de feuilles d’Aloe buettneri A. Berger (Liliacées), Dicliptera verticillata GJH Amshoff (Acanthacées), Hibiscus macranthus Hochst ex A-rich (Malvacées), Justicia Insularis T. Anders (Acanthacées), sur certains paramètres biochimiques et physiologiques de la reproduction chez la rate. Thèse de Doctorat 3e cycle en biochimie. Université de Yaoundé I. 154p.

  • Telefo, P.B.; Moundipa, P.F.; Tchouanguep, F.M. (2001): Influence de l’extrait aqueux de feuilles d’Aloe buettneri, Dicliptera verticillata, Hibiscus macranthus, Justicia insularis sur la fertilité et quelques paramètres biochimiques de la reproduction chez la rate. Revue de l’Academie des Sciences du Cameroun. Vol 1. No 3. 7p:144 - 150.


Abstract Views: 370

PDF Views: 0




  • Acute and Subacute Toxicity of Aqueous Extract of Leaves Mixture of Aloe Buettneri (Liliaceae), Dicliptera Verticillata (Acanthaeae), Hibiscus Macranthus (Malvaceae) and Justicia Insularis (Acanthaceae) on Swiss Mice and Albinos Wistar Female Rats

Abstract Views: 370  |  PDF Views: 0

Authors

K. B. Pone
Central Institute of Medicinal and Aromatic Plants, P.O.CIMAP Near Kukrail, Pinic Spot Road, Lucknow, Uttar Pradesh, India
P. B. Telefo
Department of Biochemistry, University of Dschang; Faculty of Science, P.O. Box 67 Dschang,
F. M. Tchouanguep
Head of the Laboratory of Medicinal Plants, Food science and Nutrition, University of Dschang

Abstract


Aloe buettneri (Liliaceae), Justicia insularis (Acanthaceae), Hibiscus macranthus (Malvaceae), Dicliptera verticillata (Acanthaceae) (ADHJ) are medicinal plants generally found in tropical and subtropical areas. The leaf mixture of these plants is used in the Western Region of Cameroon to increase the fertility, to regularize the menstrual cycle and to treat dysmenorrhoea or cases of infertility in women. In order to evaluate the toxicity of the mixture extract of these plants, the values of their LD50 and LD100 were determined in Swiss mice and the subacute toxicity studied in albinos Wistar female rats. The herbal drug induces changes in the physiological and biochemical parameters. These include changes in body and vital organ weights, in serum, hepatic, ovarian and uterine proteins and in AST, ALT and creatinine levels. The LD50 and LD100 in male mice were 27 and 32 g/kg respectively. The behaviour of the mice treated with the doses varying between 8 to 32 g/kg was significantly changed. The leaf mixture of the plants has significantly increased the reproductive organ weights of treated female rats. The serum level, uterine and ovarian proteins as well as the creatinine level increased significantly while the hepatic protein level decreased. The rate of A.S.T remained unchanged while the rate of A.L.T increased when animals were treated at the rate of 100 mg/kg. These results suggests on one hand that aqueous extract is not short-term poisonous but presents unfavourable effects in the long run (60 days) and on the other hand that the aqueous extract have a direct action on the reproductive organs and cause disturbances on cellular metabolism.

Keywords


Toxicity, Mice, Female rats, Biochemical Parameters

References