Indian Journal of Forensic Medicine & Toxicology

Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

Pulmonary Responses of Manufactured Ultrafine Aluminum Oxide Particles Upon Repeated Exposure by Inhalation in Rats


Affiliations
1 IIBAT, Padappai, Tamil Nadu, India
2 Pathologist, IIBAT, Padappai, Tamil Nadu, India
3 CLRI, Chennai, India
     

   Subscribe/Renew Journal


The aim of this study is to evaluate the pulmonary effects of manufactured ultra fine (10 nm) aluminum oxide particles (UFAOP) upon repeated exposure by inhalation. Rats were exposed with UFAOP in nose-only inhalation chambers at concentrations of 0, 50, 100, and 160 mg/m3 for 6h per day on 5 days. Bronchoalveolar lavage analysis showed pulmonary inflammation and cytotoxicity. Histopathology of lungs showed particle induced cytotoxicity and inflammation characterized by mononuclear inflammatory cell infiltration. Lung burden data showed less percentage of clearance and higher biological half time for UFAOP. Based on our results we conclude that repeated inhalation exposure of UFAOP induced concentration dependant toxicity to lungs of rats at different time points of post exposure.

Keywords

UFAOP Inhalation Inflammation Nanoparticles
Subscription Login to verify subscription
User
Notifications
Font Size


  • Bermudez E, Mangum J B, Wong BA et al. Pulmonary responses of mice, rats, and hamsters to sub chronic inhalation of ultra fine titanium dioxide particles. Toxicol. Sci. 2004; 77, 347–357.

  • Chen L, Yokel RA, Hennig B, Toborek M. Manufactured aluminum oxide nano particles decrease expression of tight junction proteins in brain vasculature. J Neuroimmune Pharmacol. 2008; 3(4):p286-95.

  • Christie MS, David BW. Characterization of nanomaterials for toxicity assessment. John Wiley & Sons, Inc. WIREs Nanomed Nanobiotechnol. 2009; 1 660–670.

  • Claire M, Lang T, William M et al. The pro-inflammatory effects of low-toxicity low-solubility particles, nanoparticles and fine particles, on epithelial cells in vitro: the role of surface area. Occup Environ Med. 2007; 64:609– 615. doi: 10.1136/ oem. 2005.024802.

  • Elizabeth O, Nitin C, Vasileios G et al. Alumina nanoparticles induce expression of endothelial cell adhesion molecules. Toxicology Letters. 2008; Volume 178, Issue 3, p160-166.

  • Lu S, Duffin R, Poland C, Daly P et al. Efficacy of Simple Short-Term in Vitro Assays for Predicting the Potential of Metal Oxide Nanoparticles to Cause Pulmonary Inflammation. Environmental Health Perspectives.2009; volume 117 (2): p241-247.

  • Muller J, Huaux F, Moreau N et al. Respiratory toxicity of multi-wall carbon nanotubes. Toxicol. Appl. Pharm. 2005;207, 221–231.

  • Maynard AD, Aitken RJ, Butz T et al. Safe handling of nanotechnology. Nature 2006; 444:267–269.

  • Morrow, P. E. Dust overloading of the lungs: Update and appraisal. Toxicol. Appl. Pharmacol. 1992;113:1-12.

  • Morrow, P. Possible mechanism to explain dust overloading of the lungs. Fundam Appl Toxicol. 1988;10:369-384.

  • Pauluhn J. Pulmonary Toxicity and Fate of Agglomerated 10 and 40 nm Aluminum Oxy hydroxides following 4-Week Inhalation Exposure of Rats: Toxic Effects are determined by Agglomerated, not Primary Particle Size. Toxicological Sciences.2009; 109(1):p152-167.

  • Pauluhn J. Retrospective analysis of 4-week inhalation studies in rats with focus on fate and pulmonary toxicity of two nanosized aluminum oxyhydroxides (boehmite) and pigment-grade iron oxide (magnetite): the key metric of dose is particle mass and not particle surface area. Toxicology.2009; 259(3): p140-8.

  • Park EJ, Cho WS, Jeong J et al. Induction of inflammatory responses in mice treated with cerium oxide nanoparticles by intratracheal instillation. J. Health Sci. 2010; 56, 387-396.

  • Renwick LC, Brown D, Clouter A, Donaldson K. Increased inflammation and altered macrophage chemotactic responses caused by two ultrafine particle type. Occupational Environmental Medicine. 2004; 61: p442–447.

  • Service RF. Nanotoxicology. Nanotechnology grows up. Science.2004; 2304:1732– 1734.

  • Vorbrodt AW, Dobrogowska DH, Lossinsky AS. Ultracytochemical studies of the effects of aluminum on the blood-brain barrier of mice. J Histochem Cytochem.1994; 42:203–212.


Abstract Views: 356

PDF Views: 0




  • Pulmonary Responses of Manufactured Ultrafine Aluminum Oxide Particles Upon Repeated Exposure by Inhalation in Rats

Abstract Views: 356  |  PDF Views: 0

Authors

P. V. Rajsekhar
IIBAT, Padappai, Tamil Nadu, India
G. Selvam
Pathologist, IIBAT, Padappai, Tamil Nadu, India
A. Goparaju
IIBAT, Padappai, Tamil Nadu, India
P. Balakrishna Murthy
IIBAT, Padappai, Tamil Nadu, India
P. Neelakanta Reddy
CLRI, Chennai, India

Abstract


The aim of this study is to evaluate the pulmonary effects of manufactured ultra fine (10 nm) aluminum oxide particles (UFAOP) upon repeated exposure by inhalation. Rats were exposed with UFAOP in nose-only inhalation chambers at concentrations of 0, 50, 100, and 160 mg/m3 for 6h per day on 5 days. Bronchoalveolar lavage analysis showed pulmonary inflammation and cytotoxicity. Histopathology of lungs showed particle induced cytotoxicity and inflammation characterized by mononuclear inflammatory cell infiltration. Lung burden data showed less percentage of clearance and higher biological half time for UFAOP. Based on our results we conclude that repeated inhalation exposure of UFAOP induced concentration dependant toxicity to lungs of rats at different time points of post exposure.

Keywords


UFAOP Inhalation Inflammation Nanoparticles

References