Indian Journal of Geo-Marine Sciences

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Extraction and Correlation of Total Phenolic and Flavonoid Contents in Seaweeds Collected from Rameshwaram during Pre- and Post- Monsoon Period Using Different Solvent Systems with their Antioxidant Activity


Affiliations
1 Sathyabama Institute of Science and Technology, Rajiv Gandhi Salai, Chennai – 600 119, India
2 Centre for Ocean Research, Sathyabama Institute of Science and Technology, Rajiv Gandhi Salai, Chennai – 600 119, India
3 Department of Chemistry, Sathyabama Institute of Science and Technology, Rajiv Gandhi Salai, Chennai – 600 119, India
 

The present study was carried out to compare and correlate the phenolic and flavonoid contents of Turbinaria sp., Sargassum sp. and Gracilaria sp. extracted using different solvents including methanol, isopropyl alcohol, acetone, acetonitrile, ethyl acetate and hexane, and elucidated for their anti-oxidant activity. The total phenolic and flavonoid contents of the solvent extracts were determined using the Folin-Ciocalteau assay and aluminium chloride colorimetric assay with gallic acid and quercetin as standards, respectively. The anti-oxidant activity in phenolic and flavonoid content was also estimated by phosphomolybdenum method and was compared with gallic acid and quercetin standard. The quantitative analysis of flavonoid content reveals that methanolic extract of Sargassum sp. (9.56±0.38 mg QE/g during pre-monsoon and 9.44±0.48 mg QE/g during post-monsoon season); acetone extract of Gracilaria sp. (2.16±0.11 mg QE/g during pre-monsoon and 2.12±0.07 mg QE/g during post-monsoon season) and methanolic extract of Turbinaria sp. (4.11±0.12 mg QE/g during pre-monsoon and 4.22±0.15 mg QE/g during post-monsoon season) had higher concentration of flavonoid content. However, the quantitative analysis of phenolic content was found to be lower in all the seaweed extracts as compared to flavonoids. The anti-oxidant activity of a phenolic content and flavonoid content were also found to be correlated. The findings of the current study conclusively demonstrate the content of phenolic and flavonoid compounds significantly correlate with anti-oxidant activity.

Keywords

Anti-Oxidant Activity, Flavonoids, Phenolic Compounds, Seaweeds.
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  • Kim S & Wijesekara I, Development and biological activities of marine-derived bioactive peptides, A review, J Funct Foods, 2 (1) (2010) 1-9.

  • Cavalcante L H A, Da Matta C B B, De Araújo M V, Barbosa-Filho J M, De Lira D P, et al., Antinociceptive and anti-inflammatory activities of crude methanolic extract of red alga Bryothamnion triquetrum, Mar Drugs, 10 (9) (2012) 1977-1992.

  • Heo S-J, Hwang J-Y, Choi J-I, Han J-S, Kim H-J, et al., Diphlorethohydroxycarmalol isolated from Ishige okamuraea brown algae a potent α-glucosidase α-amylase inhibitor alleviates postprandial hyperglycemia in diabetic mice, Eur J Pharmacol, 615 (1-3) (2009) 252-256.

  • Sen S K, Gupta M, Mazumder U K, Haldar P K, Saha P, et al., Antitumor activity of Citrus maxima (Burm.) Merr. Leaves in Ehrlich's ascites carcinoma Cell-Treated Mice, Int Scholar Res Netw Pharmacol, 2011 (2011) 1-4.

  • Sandeep R, Arun K, Jugran A B, Asutosh B, Indra D, et al., Anti-oxidant and anti-microbial properties of some ethno-therapeutically important medicinal plants of Indian Himalayan Region, 3 Biotech, 6 (2016) p. 154.

  • Farghl A A, Al-Hasawi Z M & El-Sheekh M M, Assessment of Antioxidant Capacity and Phytochemical Composition of Brown and Red Seaweeds Sampled off Red Sea Coast, Appl Sci, 11 (23) (2021) p. 11079.

  • Kong C S, Kim J A, Yoon N Y, Kim S-K, et al., Induction of apoptosis by phloroglucinol derivative from Ecklonia cavain MCF-7 human breast cancer cells, Food Chem Toxicol, 47 (7) (2009) 1653-1658.

  • Li Y, Lee S, Le Q, Kim M-M & Kim S-K, Anti-allergic effects of phlorotannins on histamine release via binding inhibition between IgE and Fc RI, J Agr Food Chem, 56 (24) (2008) 12073-12080.

  • Heo S J, Park E J, Lee K W & Jeon Y-J, Antioxidant activities of enzymatic extracts from brown seaweeds, Bio Resour Technol, 96 (14) (2005) 1613-1623.

  • Park P, Heo S, Park E, Kim S-K, Byun H-G, Jeon B-T, et al., Reactive oxygen scavenging effect of enzymatic extracts from Sargassum thunbergii, J Agr Food Chem, 53 (17) (2005) 6666-6672.

  • Zou Y, Qian Z, Li Y, Kim M M, Lee S H, et al., Antioxidant effects of phlorotannins isolated from Ishige okamuraein free radical mediated oxidative systems, J Agr Food Chem, 56 (16) (2008) 7001-7009.

  • Kim M, Rajapakse N & Kim S, Anti-inflammatory effect of Ishige okamuraeethanolic extract via inhibition of NFB transcription factor in RAW 264.7 cells, Phytother Res, 23 (5) (2009) 628-634.

  • Maeda H, Hosokawa M, Sashima T & Miyashita K, Dietary combination of fucoxanthin and fish oil attenuates the weight gain of white adipose tissue and decreases blood glucose in obese/diabetic KK-Ay Mice, J Agr Food Chem, 55 (19) (2007) 7701-7706.

  • Tsukui T, Konno K, Hosokawa M, Maeda H, Sashima T, et al., Fucoxanthin and fu-coxanthinol enhance the amount of docosahexaenoic acid in the liver of KKAy obese/diabetic mice, J Agr Food Chem, 55 (13) (2007) 5025-5029.

  • Kong C, Kim J, Ahn B, Vo T S, Yoon N Y, et al., 1-(3,5-Dihydroxyphenoxy)-7-(2,4,6-trihydroxyphenoxy)-2,4,9-trihydroxydibenzo-1,4-dioxin inhibits adipocyte differentiation of 3T3-L1 fibro-blasts, Mar Biotechnol, 12 (3) (2010) 299-307.

  • Zarros A, In which cases is neuro protection useful? Adv Altern Think Neuro Sci, 1 (S1) (2009) 3-5.

  • Wichi H P, Enhanced tumor development by butylated hydroxyanisole (BHA) from the properties of effect on fure stomach and oesophagel aquamoua epithelium, Food Chem Toxicol, 26 (2007) 727-723.

  • Ely R, Supriya T & Naik C G, Antimicrobial activity of marine organisms collected off the coast of South East India, J Exper Mar Biol Ecol, 309 (2004) 121–127.

  • Duan X J, Zhang W W, Li X M & Wang B G, Evaluation of antioxidant property of extract and fractions obtained from a red alga, Polysiphonia urceolata, Food Chem, 95 (2006) 37-43.

  • Kuda T T, Sunekawa M, Goto H & Araki Y, Antioxidant properties of four edible algae harvested in the Noto Peninsula, Japan J Food Compos Anal, 18 (2005) 625-633.

  • Lim S N, Cheung P C K, Ooi V E C & Ang P O, Evaluation of tioxidative activity of extracts from brown seaweed, Sargassum siliquastrum, J Agric Food Chem, 50 (2002) 3862-3866.

  • Park P J, Shahidi F & Jeon Y J, Antioxidant activities of enzymatic extracts from and edible seaweed Sargassum horneri using ESR spectroscopy, J Food Lipids, 11 (2004) 15-27.

  • Chakraborthy K, Lipton A P, Paulraj R & Vijayan K K, Antibacterial diterpernoids of Ulva fasciata Delile from South-western coast of Indian Peninsula, Food Chem, 119 (2010) 1399–1408.

  • Yoshie Y, Wang W, Petillo D & Suzuki T, Distribution of catechins in Japanese seaweeds, Fish Sci, 66 (2000) 998–1000.

  • Sandeep R, Arun K, Jugran A B, Asutosh B, Indra D, et al., Anti-oxidant and anti-microbial properties of some ethno-therapeutically important medicinal plants of Indian Himalayan Region, 3 Biotech, 6 (2016) p. 154.

  • Santoso J, Yoshie Y & Suzuki T, The distribution and profile of nutrients and catechins of some Indonesian seaweeds, Fish Sci, 68 (2002) 1647–1648.

  • Yoshie Stark Y, Hsieh Y P & Suzuki T, Distribution of flavonoids and related compounds from seaweeds in Japan, J Tokyo Univ Fish, 89 (2003) 1–6.

  • Hodgson J M & Croft K D, Dietary flavonoids: effects on endothelial function and blood pressure, J Sci Food Agric, 86 (2006) 2492–2498.

  • Halliwell B, Oxidative stress and cancer: have we moved forward? Biochem J, 401 (2007) 1–11.

  • Yan S & Asmah R, Comparison of total phenolic contents and antioxidant activities of turmeric leaf, pandan leaf and torch ginger flower, Int Food Res J, 17 (2010) 417–423.

  • Rice-Evans C A, Miller N J & Paganga G, Structure-antioxidant activity relationships of flavonoids and phenolic acids, Free Radic Biol Med, 20 (1996) 933-956.

  • Sousa H G, Uchoa V T, Cavalcanti S M G, de Almeida P M, Chaves M H, et al., Phytochemical screening, phenolic and flavonoid contents, antioxidant and cytogenotoxicity activities of Combretum leprosum Mart. (Combretaceae), J Tox Envtl Health, Part A, 84 (10) (2021) 399-417.

  • Rattaya S, Benjakul S & Prodpran T, Extraction, antioxidative, and antimicrobial activities of brown seaweed extracts, Turbinaria ornata and Sargassum polycystum, grown in Thailand, Int Aquatic Res, 7 (1) (2015) 1-16.

  • Hertog M G L, Hollman P C H & Katan M B, Content of potentially anticarcinogenic flavonoids of 28 vegetables and fruits commonly in the Netherlands, J Agri Food Chem, 40 (1992) 2379-2383.

  • Lanfer-Marquez U M, Barros R M C & Sinnecker P, Antioxidant activity of chlorophylls and their derivatives, Food Res Int, 38 (2005) 885–891.

  • Ragazzi E & Veronese G, Quantitative analysis of phenolic compounds after thin-layer chromatographic separation, J Chromatography, 77 (1973) 369-375.

  • Xia L R, Wang Q, Zhuguo H, Li L, Shu B K, et al., Simultaneous determination of 10 major flavonoids in Dalbergi Oderifera by high performance liquid chromatography, J Pharm Biomed Anal, 39 (3-4) (2005) 469-476.

  • Morales L, Acero N, Galán A, Perez-Garcia C, Alguacil L F, et al, Bioactive properties of Tynanthus panurensis (Bureau) Sanwith bark extract, the Amazonian “clavo huasca”, J Medicinal Food, 14 (9) (2011) 939-943.

  • Zhishen J, Mengcheng T & Jianming W, The determination of flavonoid contents in mulberry and their scavenging effects on Superoxide radicals, Food Chem, 64 (1999) 555-559.


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  • Extraction and Correlation of Total Phenolic and Flavonoid Contents in Seaweeds Collected from Rameshwaram during Pre- and Post- Monsoon Period Using Different Solvent Systems with their Antioxidant Activity

Abstract Views: 130  |  PDF Views: 82

Authors

K. M. Smita
Sathyabama Institute of Science and Technology, Rajiv Gandhi Salai, Chennai – 600 119, India
L. Stanley Abraham
Centre for Ocean Research, Sathyabama Institute of Science and Technology, Rajiv Gandhi Salai, Chennai – 600 119, India
S. Sunitha
Department of Chemistry, Sathyabama Institute of Science and Technology, Rajiv Gandhi Salai, Chennai – 600 119, India
R. Vasantharaja
Centre for Ocean Research, Sathyabama Institute of Science and Technology, Rajiv Gandhi Salai, Chennai – 600 119, India
R. Thirugnanasambandam
Centre for Ocean Research, Sathyabama Institute of Science and Technology, Rajiv Gandhi Salai, Chennai – 600 119, India

Abstract


The present study was carried out to compare and correlate the phenolic and flavonoid contents of Turbinaria sp., Sargassum sp. and Gracilaria sp. extracted using different solvents including methanol, isopropyl alcohol, acetone, acetonitrile, ethyl acetate and hexane, and elucidated for their anti-oxidant activity. The total phenolic and flavonoid contents of the solvent extracts were determined using the Folin-Ciocalteau assay and aluminium chloride colorimetric assay with gallic acid and quercetin as standards, respectively. The anti-oxidant activity in phenolic and flavonoid content was also estimated by phosphomolybdenum method and was compared with gallic acid and quercetin standard. The quantitative analysis of flavonoid content reveals that methanolic extract of Sargassum sp. (9.56±0.38 mg QE/g during pre-monsoon and 9.44±0.48 mg QE/g during post-monsoon season); acetone extract of Gracilaria sp. (2.16±0.11 mg QE/g during pre-monsoon and 2.12±0.07 mg QE/g during post-monsoon season) and methanolic extract of Turbinaria sp. (4.11±0.12 mg QE/g during pre-monsoon and 4.22±0.15 mg QE/g during post-monsoon season) had higher concentration of flavonoid content. However, the quantitative analysis of phenolic content was found to be lower in all the seaweed extracts as compared to flavonoids. The anti-oxidant activity of a phenolic content and flavonoid content were also found to be correlated. The findings of the current study conclusively demonstrate the content of phenolic and flavonoid compounds significantly correlate with anti-oxidant activity.

Keywords


Anti-Oxidant Activity, Flavonoids, Phenolic Compounds, Seaweeds.

References