Indian Journal of Geo-Marine Sciences

Open Access Open Access  Restricted Access Subscription Access

Molecular marker (RAPD and RFLP) analysis of Mullet species


Affiliations
1 Institute of Fisheries Postgraduate Studies, Tamil Nadu Dr. J. Jayalalithaa Fisheries University, Chennai, Tamil Nadu – 603103, India
2 ICAR – National Bureau of Fish Genetic Resources, Kochi Centre, Kochi, Kerala – 682 018, India

Mullet species belongs to the family, Mugilidae. Traditional taxonomic characters failed to differentiate mullet species due to the possibility of cryptic species and morphological similarity among mullet species. This study aimed to differentiate the selected mullet species using molecular markers. A total of 95 mullet samples (M. cephalus, P. macrolepis, and P. tade) were collected from Tamil Nadu and Kerala. For this study, 5 RAPD primers (OPA1, OPA10, OPA11, OPA15, and OPA16) were chosen from a set of 30 universal primers. The Nei’s unbiased genetic distance was calculated with the help of the software “POPGEN version 1.32”. The genetic distance between P. tade and P. macrolepis was lower, while the distance between M. cephalus and P. tade was greater, measuring 43.37 %. For the PCR-RFLP marker, the restriction enzyme was chosen based on in-silico analysis. The Alu I enzyme displayed species-specific patterns in M. cephalus at 190 bp, 200 bp, and 210 bp for the 16S rRNA gene.

Keywords

16s rRNA, Markers, Mullet, RAPD, RFLP, Taxonomy
User
Notifications
Font Size

Abstract Views: 25




  • Molecular marker (RAPD and RFLP) analysis of Mullet species

Abstract Views: 25  | 

Authors

I Vidhya
Institute of Fisheries Postgraduate Studies, Tamil Nadu Dr. J. Jayalalithaa Fisheries University, Chennai, Tamil Nadu – 603103, India
N Felix
Institute of Fisheries Postgraduate Studies, Tamil Nadu Dr. J. Jayalalithaa Fisheries University, Chennai, Tamil Nadu – 603103, India
A Kathirvelpandian
ICAR – National Bureau of Fish Genetic Resources, Kochi Centre, Kochi, Kerala – 682 018, India
E Suresh
Institute of Fisheries Postgraduate Studies, Tamil Nadu Dr. J. Jayalalithaa Fisheries University, Chennai, Tamil Nadu – 603103, India

Abstract


Mullet species belongs to the family, Mugilidae. Traditional taxonomic characters failed to differentiate mullet species due to the possibility of cryptic species and morphological similarity among mullet species. This study aimed to differentiate the selected mullet species using molecular markers. A total of 95 mullet samples (M. cephalus, P. macrolepis, and P. tade) were collected from Tamil Nadu and Kerala. For this study, 5 RAPD primers (OPA1, OPA10, OPA11, OPA15, and OPA16) were chosen from a set of 30 universal primers. The Nei’s unbiased genetic distance was calculated with the help of the software “POPGEN version 1.32”. The genetic distance between P. tade and P. macrolepis was lower, while the distance between M. cephalus and P. tade was greater, measuring 43.37 %. For the PCR-RFLP marker, the restriction enzyme was chosen based on in-silico analysis. The Alu I enzyme displayed species-specific patterns in M. cephalus at 190 bp, 200 bp, and 210 bp for the 16S rRNA gene.

Keywords


16s rRNA, Markers, Mullet, RAPD, RFLP, Taxonomy