Indian Journal of NanoScience

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In Vitro Cytotoxicity Studies of Cerium Oxide Nanoparticles


Affiliations
1 Department of Zoology, S.I.V.E.T College, Chennai-600073, India
2 GLR Laboratories Pvt Ltd, MK7 8HG, United Kingdom
3 GLR Laboratories Pvt Ltd, Chennai-600060, India
 

Background/Objectives: Cerium oxide nanoparticles (CeO2-NP), a member of the lanthanide series of rare metal oxide, have beneficial property like radicals scavenging and being examined as a potent candidate for many biomedical applications. The aim of the study is to investigate certain key in vitro cytotoxicity endpoints using CeO2- NP.

Methods/Statistical analysis: The techniques applied include, characterization, MTT assay, LDH release, ATP measurement and Caspase 3/7 activity assay. The results were subjected to null hypothesis testing to determine its significance.

Findings: CeO2-NPshowed mild cytotoxicity with a loss of MTT reactivity and depletion in ATP production. CeO2-NP treatment also resulted in an increase in Caspase3/7 activity and leakage of LDH in the tested cell line suggesting that it induces apoptosis via release of reactive oxygen species or through the physical damage of the cell membrane.

Improvements/Applications: Thefindings add base line information to the biocompatibility and toxicity data sheet on CeO2-NP. The results will also be beneficial in risk assessing CeO2-NP based medical devices.


Keywords

Cerium Oxide Nanoparticles, Cytotoxicity, L-929 Cells.
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  • K.Reinhardt, H.Winkler. Cerium mischmetal, cerium alloys, and cerium compounds. In: Ullmann'sencyclopedia of industrial chemistry. Weinheim, Germany: Wiley-VCH. 2002; 7, 285-300.

  • J.B.Hedrick. Rare earths: U.S. Geological Survey Minerals Yearbook, 2002; I, 60.1-60.16.

  • C.Walkey, S.Das, S.Seal, J.Erlichman, K.Heckman, L.Ghibelli, E.Traversa, J.F.McGinnis, T.William. Catalytic properties and biomedical applications of cerium oxide nanoparticles. Environmental Science: Nano. 2015; 2, 33-53.

  • N.Pourkhalili, A.Hosseini, A.Nili-Ahmadabadi, S.Hassani, M.Pakzad, M.Baeeri, A.Mohammadirad, M.Abdollahi. Biochemical and cellular evidence of the benefit of a combination of cerium oxide nanoparticles and selenium to diabetic rats. World Journal of Diabetes.2011; 2, 204-210.

  • J.Niu, A.Azfer, L.M.Rogers, X.Wang, P.E.Kolattukudy. Cardioprotective effects of cerium oxide nanoparticles in a transgenic murine model of cardiomyopathy. Cardiovascular Research. 2007; 73, 549-559.

  • I. Celardo, J.Z. Pedersen, E. Traversa, GhibelliL . Pharmacological potential of cerium oxide nanoparticles.Nanoscale. 2011;3(4),1411-1420.

  • K.Chaudhury, K.N.Babu, A.K.Singh, S.Das, A.Kumar, S.Seal. Mitigation of endometriosis using regenerative cerium oxide nanoparticles. Nanomedicine. 2013; 9 (3),439-448.

  • S.Das, J.M.Dowding, K.E.Klump, J.F.McGinnis, W.Self, S.Seal. Cerium oxide nanoparticles: applications and prospects in nanomedicine. Nanomedicine (Lond). 2013;8(9),1483-1508.

  • A.Y.Estevez, S.Pritchard, K.Harper, J.W.Aston, A.Lynch, J.J.Lucky, J.S.Ludington, P.Chatani, W.P.Mosenthal, J.C.Leiter, S.Andreescu, J.S.Erlichman. Neuroprotective mechanisms of cerium oxide nanoparticles in a mouse hippocampal brain slice model of ischemia. Free Radical Biology and Medicine. 2011; 51(6), 1155-1163.

  • L.L.Wong, J.F.McGinnis. Nanoceria as bona fide catalytic antioxidants in medicine: what we know and what we want to know. Advances in Experimental Medicine and Biology.2014; 801,821-828.

  • M.S.Wason, J.Zhao. Cerium oxide nanoparticles: potential applications for cancer and other diseases, Review Article. American Journal of Translational Research. 2013; 5(2),126-131.

  • A.Y.Estevez, J.S.Erlichman. The potential of cerium oxide nanoparticles (nanoceria) for neurodegenerative disease therapy.Nanomedicine. 2014; 9(10), 1437-1440.

  • Biological Evaluation of Medical Devices – Part 1, Evaluation and testing within a risk management process, ISO 10993-1, 2009/Cor 1:2010.

  • B.Brabu, S.Haribabu, M.Revathy, S.Anitha, M.Thangapandiyan, K.R.Navaneethakrishnan, C.Gopalakrishnan, S.S.Murugan, T.S.Kumaravel. Biocompatibility studies on lanthanum oxide nanoparticles. Toxicology Research. 2015; 15, 43-30.

  • X.Zhang, S.Wang, M.Liu, J.Hui, B.Yang, L.Tao, Y.Wei. Surfactant-dispersed nanodiamond: biocompatibility evaluation and drug delivery applications. Toxicolology Research. 2013; 2, 335-342.

  • X.Zhang, M.Liu, X.Zhang, F.Deng, C.Zhou, J.Hui, W.Liu, Y.Wei. Interaction of tannic acid with carbon nanotubes: enhancement of dispersibility and biocompatibility, Toxicology Research. 2015; 4, 160-168.

  • J.H.Liu, T.Wang, H.Wang, Y.Gu, Y.Xu, H.Tang, G.Jia, Y.Liu. Biocompatibility of graphene oxide intravenously administrated in mice—effects of dose, size and exposure protocols. Toxicology Research. 2015; 4, 83-91.

  • Biological Evaluation of Medical Devices – Part 5: Tests for In vitro Cytotoxicity, ISO 10993-5, 2009.

  • A.R.Asati. Synthesis of biocompatible antioxidant polymer coated cerium oxide nanoparticles, its oxidase like behavior and cellular uptake studies. Thesis, University of Central Florida, Orlando, Florida. 2009, 156-158.


Abstract Views: 354

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  • In Vitro Cytotoxicity Studies of Cerium Oxide Nanoparticles

Abstract Views: 354  |  PDF Views: 207

Authors

V. Kalyanaraman
Department of Zoology, S.I.V.E.T College, Chennai-600073, India
T. S. Kumaravel
GLR Laboratories Pvt Ltd, MK7 8HG, United Kingdom
S. S. Murugan
GLR Laboratories Pvt Ltd, Chennai-600060, India

Abstract


Background/Objectives: Cerium oxide nanoparticles (CeO2-NP), a member of the lanthanide series of rare metal oxide, have beneficial property like radicals scavenging and being examined as a potent candidate for many biomedical applications. The aim of the study is to investigate certain key in vitro cytotoxicity endpoints using CeO2- NP.

Methods/Statistical analysis: The techniques applied include, characterization, MTT assay, LDH release, ATP measurement and Caspase 3/7 activity assay. The results were subjected to null hypothesis testing to determine its significance.

Findings: CeO2-NPshowed mild cytotoxicity with a loss of MTT reactivity and depletion in ATP production. CeO2-NP treatment also resulted in an increase in Caspase3/7 activity and leakage of LDH in the tested cell line suggesting that it induces apoptosis via release of reactive oxygen species or through the physical damage of the cell membrane.

Improvements/Applications: Thefindings add base line information to the biocompatibility and toxicity data sheet on CeO2-NP. The results will also be beneficial in risk assessing CeO2-NP based medical devices.


Keywords


Cerium Oxide Nanoparticles, Cytotoxicity, L-929 Cells.

References