Open Access Open Access  Restricted Access Subscription Access

Green Synthesis, and Antimicrobial Activity of Silver Nanoparticles from the Medicinal Plant Vernonia amygdalina


Affiliations
1 Dhanapalan College of Arts and Science, Kelambakkam, Chennai-603103, India
2 JBAS College for Women, Teynampet, Chennai-600018, India
3 Dept of Applied Sciences, PNG, University of Technology, Papua New Guinea, Papua New Guinea
 

Silver nanoparticles of different concentrations were synthesized from the medicinal plant Vernonia amygdalina biologically, as this technique is cost effective and environment friendly. The characterization of the silver nanoparticles was analysed by the UV-Vis Spectrophotometer, Transmission Electron Microscopy (TEM), Scanning Electron Microscopy, X-Ray Diffraction (XRD), and Fourier Transform Infra Red (FTIR). The absorption spectra of silver nanoparticles studied using the UV-Vis spectroscopy, had an absorbance peak at 475nm, in all the three different concentrations (1mM, 3mM and 5mM) of silver nanoparticles. The silver nanoparticles have a 50-70nm size range and appeared to be dominantly spherical and were occasionally triangular. The XRD pattern revealed that these silver nanoparticles have a crystalline nature. The antimicrobial activity of these nanoparticles was studied against E.coli, S.aureus, P.aeuruginosa, and C.albicans. They appeared to have satisfactory inhibitions against the four mentioned microorganisms. Among the different concentrations used in the study, the 3mM (20mg/500μl distilled water) appeared to have the highest sensitivity.

Keywords

Amygdalina Vernonia, Bioreduction, Silver Nanoparticles, Microorganisms
User
Notifications

  • Vyom Parashar, Rashmi Parashar, Bechan Sharma, Avinash C.Pandey, Digest Journal of [2009]. Nanomaterials and Biostructures, 4(1),45 -50
  • N.Roy and A.Barik,Int.J.Nanotech and Appl,4(2):95- 101[2001].
  • Upendra Kumar Parashar,Preeti S.Saxena,Anchal Srivastava, Digest Journal of Nanomaterials and Biostruct ures,4(1),159-166[2009].
  • D.S. Goodsell Bionanotechnology: Lessons from Nature. John Wiley & Sons Inc.Publication (2004).
  • E.Mayes, A.Bewick,D Gleeson,J Hoinville,R Jones,IEEE Transactions on Magnetics 39(2), 624(2003).
  • Belly, R. T., and G. C. Kydd. 1982. Silver resistance in microorganisms. Dev. Ind. Microbiol. 23:567-577.
  • Joseph Wang.,Analytica Chimica Acta 500, 247(2003).

Abstract Views: 654

PDF Views: 767




  • Green Synthesis, and Antimicrobial Activity of Silver Nanoparticles from the Medicinal Plant Vernonia amygdalina

Abstract Views: 654  |  PDF Views: 767

Authors

Yamini Sudha Lakshmi
Dhanapalan College of Arts and Science, Kelambakkam, Chennai-603103, India
Fouzia Banu
JBAS College for Women, Teynampet, Chennai-600018, India
S. Gopalakrishnan
Dept of Applied Sciences, PNG, University of Technology, Papua New Guinea, Papua New Guinea

Abstract


Silver nanoparticles of different concentrations were synthesized from the medicinal plant Vernonia amygdalina biologically, as this technique is cost effective and environment friendly. The characterization of the silver nanoparticles was analysed by the UV-Vis Spectrophotometer, Transmission Electron Microscopy (TEM), Scanning Electron Microscopy, X-Ray Diffraction (XRD), and Fourier Transform Infra Red (FTIR). The absorption spectra of silver nanoparticles studied using the UV-Vis spectroscopy, had an absorbance peak at 475nm, in all the three different concentrations (1mM, 3mM and 5mM) of silver nanoparticles. The silver nanoparticles have a 50-70nm size range and appeared to be dominantly spherical and were occasionally triangular. The XRD pattern revealed that these silver nanoparticles have a crystalline nature. The antimicrobial activity of these nanoparticles was studied against E.coli, S.aureus, P.aeuruginosa, and C.albicans. They appeared to have satisfactory inhibitions against the four mentioned microorganisms. Among the different concentrations used in the study, the 3mM (20mg/500μl distilled water) appeared to have the highest sensitivity.

Keywords


Amygdalina Vernonia, Bioreduction, Silver Nanoparticles, Microorganisms

References