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Authors
Affiliations
1 B.Sc. in Biology, Department of Microbiology, Science College, Kerbala City, IQ
2 Ph.D. in Biology, Department of Microbiology, College of Medicine, University of Kerbala City, IQ
3 Ph.D. in Genetic Engineering and Biotechnology, Department of Clinical Laboratory Sciences, College of Pharmacy, University of Kerbala, IQ
Source
Indian Journal of Public Health Research & Development, Vol 11, No 2 (2020), Pagination: 2244-2247
Abstract
Background: Escherichia. coli consider as commensal bacterium that can be inhabitant in the small intestinal micro-biota of warm-blooded animals, Urinary tract infections cause by Uropathogenic Escherichia coli (UPEC) Are among the most branded infectious diseases in humans. Due to their recurrently incidence in the community and nosocomial infection and evolution of resistance to ordinarily recommended antimicrobial agents. Objective: The aim of current study was Investigation of FimH gene occurrence a virulence factor in Escherichia coli. Materials and Method: Sixty urine samples of women with urinary tract infection symptoms. In addition, samples from different environmental places involving poultry, knacker shops, drainage water, fresh water and sewage water were collected during 22ed November 2017 to 28ed February 2018. Twenty one clinical and twenty three environmental isolates of Escherichia coli. The data were analyzed by using the statistical package social system (SPSS). Results: The biofilm formation were 14(66.6%) strong, 7(33.30%) moderate and weak biofilm producers not exit in clinical isolates, while 13(41%) strong, 8(34.70%) moderate and 2(8.60%) for weak biofilm formation in environmental isolates. Moreover, PCR assay of FimH was applied. So,20 isolates (95.23%) were positive results for clinical isolates and 17 isolates (73.9%) positive results for environmental Conclusion: Uropathogenic E. coli had the higher occurrence rate for fimbriae gene (Fim H) compared with environmental isolates,Stronger biofilm formation by clinical E. coli isolates than by environmental isolates. There is a significant correlation between biofilm formation and probability of Fim H occurrence in both clinical and environmental isolates.
Keywords
Escherichia coli, FimH gene, Biofilm formation.
