Indian Journal of Public Health Research & Development

Arunava Das ¹, J. Bindhu ¹, M. Bharath ¹, Nithin Johnson , M. Jeevanantham

Affiliations
1 Molecular Diagnostics and Bacterial Pathogenomics Research Laboratory, Department of Biotechnology, Bannari Amman Institute Technology, Sathyamangalam, Erode District, Tamil Nadu, IN

Abstract

Plants have been used as natural medicines since the dawn of human kind. This research was focused on phytochemical analysis of Punica grantum peel extract consist of ethanol: chloroform (99:1) as solvent and its constituents were molecularly characterized by GCMS analysis and its ability as an antioxidant, anticancer against K562 cell and antibacterial against some food borne pathogens which comprises of 5 gram-positive and 3-gram-negative bacteria. The phytochemical analysis was performed biochemically and results reveals the presence of secondary metabolites such as Flavonoids, Alkaloids, Tannins, Saponins, etc. and absence of glycosides. The extract was tested against both gram-positive and gram-negative bacteria by agar well diffusion method and its results have shown that the streptococcus faecalis have shown highest susceptibility against the extract. Its antioxidant potential was quantitatively determined by DPPH assay and results showed high range of antioxidant present in the extract with IC50 as 504.9μg/ml. The cytotoxicity assay was performed with peel extract of different concentration against K562 cancer lines which is a myelogenous leukemia cell line. However, the cytotoxicity is less when compared with cell viability and its effects were mild against the cancer cells.

Keywords

Punicagranatum, DPPH, GCMS, K562 Cancer Cells (Myelogenous Leukemia).

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Arunava Das ¹, J. Bindhu ², P. Deepesh ³, G. Shanmuga Priya ³, S. Soundariya ³

Affiliations
1 Associate Professor, Molecular Diagnostics and Bacterial Pathogenomics Research Laboratory, Department of Biotechnology, Bannari Amman Institute Technology, Sathyamangalam, Erode District, Tamil Nadu, IN
2 Research Associate cum Assistant Professor, Molecular Diagnostics and Bacterial Pathogenomics Research Laboratory, Department of Biotechnology, Bannari Amman Institute Technology, Sathyamangalam, Erode District, Tamil Nadu, IN
3 III Year Biotech Students, Molecular Diagnostics and Bacterial Pathogenomics Research Laboratory, Department of Biotechnology, Bannari Amman Institute Technology, Sathyamangalam, Erode District, Tamil Nadu, IN

Abstract

Banana tree being one of the most economically important tree worldwide is cut once the fruits are harvested. However other parts of the tree are used in medicine worldwide. In this study, the flower extract of Musa acuminatais extracted by maceration with methanol and the phenolic and aliphatic compounds present in the flower extract is identified using GC-MS analysis. Phytochemical screening of the banana extract showed the presence of alkaloids, phlobatanning, triterpanoids, flavanoids, lipids, steroids and terpenoids. Potent antibacterial activity is observed from the flower extract against the tested gram positive and negative bacteria. Anticancer activity of the flower extract is assessed on the cervical cancer cell line HeLa. MTT assay is used to evaluate the antiproliferative effects. This study concluded that the economically important Musa acuminatais widely used in anticancer studies worldwide.

Keywords

Musa acuminata, Phenolic Compounds, GC-MS Analysis, Phytochemical Screening, Antibacterial Activity, MTT Assay.

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