Indian Journal of Public Health Research & Development

Ghydaa H. Aljeboury ¹, Mohsen Hashim Risan ², Rebah Najah Algafari ¹

Affiliations
1 Biotechnology Research Center, Al-Nahrain University, IQ
2 College of Biotechnology, Al-Nahrain University, IQ

Abstract

A total of (130) samples of stomach ulcer were collected from patients suffering from peptic ulcer who visited three different hospitals in Baghdad. The results showed that after bacterial culturing on the MacConkey agar and Columbia agar and selective media of Helicobacter Pylori bacteria under(5% O₂, 10% CO₂, and 85% N₂), detected that 45 isolate were belong Helicobacter pylori that represent(35%). After testing the sensitivity test against Helicobacter pylori against 10 antibiotics, the sensitivity test for 10 antibiotics demonstrated the efficacy of the antibiotic cephotaxim and rifampicin in the elimination of the bacteria. The resistance ratio was 10%. The tetracycline and duxacycline antibiotic were 10% 20% and were resistant to erythromycin and chlarthromycin30%, while the resistance to amoxicillin was 100% and the resistance ratio was 80% for both metronidazole and sprofluxacin, while gentamycin had a resistance ratio of 50%.

Keywords

Helicobacter pylori, PCR, 16SrRNA.

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