International Journal of Pharmaceutical Sciences and Nanotechnology (IJPSN)

Open Access Open Access  Restricted Access Subscription Access

Antioxidant Activity of Hydroalcoholic Extract of Labisa Pumila var. Alata, an Indigenous Plant of Malaysia


Affiliations
1 Faculty of Pharmacy, AIMST University, Semeling, Bedong, Kedah,, Malaysia
 

This study was carried out to investigate the antioxidant potential of crude extracts of Labisa pumila var. Alata, a small herbaceous under shrub widely available in Malaysia. In this study, antioxidant activity was evaluated by diphenyl picryl hydrazine (DPPH) free radical assay and FRAP assay methods. In the DPPH free radical assay, different concentrations of the extract (5, 10, 25, 50 and 75 μg/ml in ethanol) were prepared and mixed with DPPH ethanol solution. After incubation, a photometric assay was performed and values were plotted. The IC50 values were calculated by a linear regression of plots and compared with IC50 of ascorbic acid as the standardantioxidant. In the FRAP assay, different concentrations of extract (20, 40, 60, 80 and 100 μg/ml) were prepared and the reducing power was estimated by a photometric assay and values were plotted. The EC50 values were calculated by a linear regression of plots and compared with the EC50 of ascorbic acid as a standard reducing agent. The study revealed that the crude extract possesses antioxidant activity.
User
Notifications
Font Size

  • Amarowicz R, Naczk M and Shahidi F (2000). Antioxidant activity of crude extracts of canola/rapeseed hulls. J. Am. Oil Chem. Soc. 77: 957–961.

  • Baumann J, Wurn G and Bruchlausen FV (1979). Prostaglandin synthetase inhibiting, O2− radical scavenging properties of some flavonoids and related phenolic compounds. NaunynSchmiedeberg’s Arch. Pharmacol. 308: R27.

  • Benzie IFF and Strain JJ (1996). The ferric reducing ability of plasma (FRAP) as a measure of ‘Antioxidant Power’: the FRAP assay. Anal. Biochem. 239: 70-76.

  • Chang LW, Yen WJ, Huang SC and Duh PD (2002). Antioxidant activities of seasame coat.Food Chem. 78: 347-354.

  • Ezumi WMF, Amrah SS, Suhaimi AWM, and Mohsin SSJ (2007). Evaluation of the female reproductive toxicity of aqueous extract of Labisia pumila Var. Alata in rats. Indian J. Pharmacol. 39 (1): 30-32.

  • Fatimah ZI, Zaiton Z, Jamaludin M and Gapor MT, Nafeeza MI, Khairul O (1998). In: Packer L and Ong SH (eds.), Biological Oxidants and Antioxidants: Molecular Mechanism and Health Effects, AOCS Press, Champaign, Illinois, Chapter 22.

  • Giri MA, Rasika D, Bhalke A, Subodh C and Pal B (2010). Gastroprotective effect of hydroalcoholic leaves extract of Pongamia pinnata. International J. of Pharma and Bio Sciences.1 (3): 1-6.

  • Halliwell B (1997). Antioxidants and human disease: a general introduction. Nutr Rev. 55: S44–S52.

  • Hatano T,Edamatsu R, Hiramatsu M, Mori A, Fujita Y and Yasuhara E (1989). Effects of tannins and related polyphenols on superoxide anion radical and on DPPH radical. Chem. Pharm. Bull. 37: 2016–2021.

  • Isabel CFR, Ferreira, Paula B, Miguel V, and Lillian B (2007). Freeradical scavenging capacity and reducing power of wild edible mushrooms from northeast Portugal: individual cap and stipe activity. Food Chem. 100: 1511–1516.

  • Limei Y, Mouming Z, Bao Y, Qiangzhong Z and Yueming J (2007). Phenolics from hull of Garcinia mangostina fruit and their antioxidant activities. Food Chemistry. 104: 176-181.

  • Liu RH (2002). Supplement quick fix fails to deliver. Food Technol. Int.1: 71‐72.

  • Narasimhan S, Shobana R and Sathya TN (2006). AntioxidantsNatural rejuvenators that heal, detoxify and provide nourishment.

  • In: Rakesh KS and Rajesh A (eds.), Herbal Drugs: a Twenty First Century Prospective, JP Brothers Medical Publishers, New Delhi, pp 548-57.

  • Nilsson J, Pillai D, Önning G, Persson C, Nilsson Å, and Åkesson B (2005). Comparison of the ABTS and FRAP methods to assess the total antioxidant capacity in extracts of fruit and vegetables. Molecular Nutrition and Food Research. 49: 239-246.

  • Norhaiza M, Maziah M and Hakiman M (2009). Antioxidative properties of leaf extracts of a popular Malaysian herb, Labisia pumila. J. Medicinal Plants Research. 3(4): 217-223.

  • Oyaizu M (1986). Studies on products of browning reactions: antioxidative activities of products of browning reaction prepared from glucosamine. Japanese J. of Nutrition. 44: 307–315.

  • Packer (1994). Methods in Enzymology: Oxygen Radicals in Biological Systems, Academic Press, San Diego. Soares JR, Dins TCP, Cunha AP, Almeida LM (1997). Antioxidant activity of some extracts of Thymus zygis. Free Radical Research 26: 469-478.

  • Wojdyło A, Oszmian J and Czemerys R (2007). Antioxidant activity and phenolic compounds in 32 selected herbs. Food Chem. 105(3): 940-949.

  • Wong CC, Li HB, Cheng KW, and Chen F (2006). A systematic survey of antioxidant activity of 30 Chinese medicinal plants using the ferric reducing antioxidant power assay. Food Chem. 97: 705-711.

  • Yen GC, Duh PD and Tsai CL (1993). Relationship between antioxidant activity and maturity of peanut hulls. J. Agric. Food Chem. 41: 67-70.


Abstract Views: 67

PDF Views: 60




  • Antioxidant Activity of Hydroalcoholic Extract of Labisa Pumila var. Alata, an Indigenous Plant of Malaysia

Abstract Views: 67  |  PDF Views: 60

Authors

Christapher Varghese
Faculty of Pharmacy, AIMST University, Semeling, Bedong, Kedah,, Malaysia
Christina Ambrose
Faculty of Pharmacy, AIMST University, Semeling, Bedong, Kedah,, Malaysia
R. Veerasamy
Faculty of Pharmacy, AIMST University, Semeling, Bedong, Kedah,, Malaysia
Pea Nai Hui
Faculty of Pharmacy, AIMST University, Semeling, Bedong, Kedah,, Malaysia
Chen Siang May
Faculty of Pharmacy, AIMST University, Semeling, Bedong, Kedah,, Malaysia

Abstract


This study was carried out to investigate the antioxidant potential of crude extracts of Labisa pumila var. Alata, a small herbaceous under shrub widely available in Malaysia. In this study, antioxidant activity was evaluated by diphenyl picryl hydrazine (DPPH) free radical assay and FRAP assay methods. In the DPPH free radical assay, different concentrations of the extract (5, 10, 25, 50 and 75 μg/ml in ethanol) were prepared and mixed with DPPH ethanol solution. After incubation, a photometric assay was performed and values were plotted. The IC50 values were calculated by a linear regression of plots and compared with IC50 of ascorbic acid as the standardantioxidant. In the FRAP assay, different concentrations of extract (20, 40, 60, 80 and 100 μg/ml) were prepared and the reducing power was estimated by a photometric assay and values were plotted. The EC50 values were calculated by a linear regression of plots and compared with the EC50 of ascorbic acid as a standard reducing agent. The study revealed that the crude extract possesses antioxidant activity.

References