International Journal of Pharmaceutical Sciences and Nanotechnology (IJPSN)

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Cytotoxic, Antioxidant and Antimicrobial Activity Of Polygonum Chinensis Linn


Affiliations
1 Department of Pharmaceutical Biotechnology, JSS College of Pharmacy, Rocklands Ootacamund, Tamil Nadu, India
 

Polygonum chinensislinn belongs to the family Polygonaceae. The plant used in this study was shade dried and extracted with methanol by a cold maceration process. Qualitiave phytochemicals estimation showed the presence of the majority of compounds including terpinoids, flavonoids, tannins, saponins and glycosides. All the extracts showed moderate inhibition ranged from 12- 14 mm against both Gram-positive and Gram-negative strains and 00-21 mm against fungal trains. In the two-fold serial dilution method, the minimum inhibitory concentration of the extract ranges between 250 – >1000 µg/ml. The leaf extract of polygonum chinensisshowed the potent activity with an IC50value of 18.59 ± 0.356 µg/ml. In DPPH, the lipid peroxidation and hydrogen peroxide method, the leaf extract showed potent antioxidant activity. Root and stem extract showed moderate antioxidant activity. All the extracts showed moderate cytotoxicity to all the five cell lines Vero, A-549, L6, BRL, 3A and L-929 with CTC50value ranging from 400-125 µg/ml. Short-term cytotoxicity studies were conducted for all the extracts against (Dalton Lymphocytes ascites) cells. The CTC50value was found to be 435 µg/ml. Thus, the extracts of P. Chinensis may possess cytotoxic, antioxidant and antimicrobial activity.

Keywords

Antimicrobial, DPPH Method, Cytotoxicity, Polygonum Chinensis, Short-Term Antitumor Studies.
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  • Cytotoxic, Antioxidant and Antimicrobial Activity Of Polygonum Chinensis Linn

Abstract Views: 68  |  PDF Views: 63

Authors

A.R. Srividya
Department of Pharmaceutical Biotechnology, JSS College of Pharmacy, Rocklands Ootacamund, Tamil Nadu, India
A. Shalom
Department of Pharmaceutical Biotechnology, JSS College of Pharmacy, Rocklands Ootacamund, Tamil Nadu, India
R. Chandrasekhar
Department of Pharmaceutical Biotechnology, JSS College of Pharmacy, Rocklands Ootacamund, Tamil Nadu, India
P. Vijayan
Department of Pharmaceutical Biotechnology, JSS College of Pharmacy, Rocklands Ootacamund, Tamil Nadu, India
V.J. Vishnuvarthtan
Department of Pharmaceutical Biotechnology, JSS College of Pharmacy, Rocklands Ootacamund, Tamil Nadu, India

Abstract


Polygonum chinensislinn belongs to the family Polygonaceae. The plant used in this study was shade dried and extracted with methanol by a cold maceration process. Qualitiave phytochemicals estimation showed the presence of the majority of compounds including terpinoids, flavonoids, tannins, saponins and glycosides. All the extracts showed moderate inhibition ranged from 12- 14 mm against both Gram-positive and Gram-negative strains and 00-21 mm against fungal trains. In the two-fold serial dilution method, the minimum inhibitory concentration of the extract ranges between 250 – >1000 µg/ml. The leaf extract of polygonum chinensisshowed the potent activity with an IC50value of 18.59 ± 0.356 µg/ml. In DPPH, the lipid peroxidation and hydrogen peroxide method, the leaf extract showed potent antioxidant activity. Root and stem extract showed moderate antioxidant activity. All the extracts showed moderate cytotoxicity to all the five cell lines Vero, A-549, L6, BRL, 3A and L-929 with CTC50value ranging from 400-125 µg/ml. Short-term cytotoxicity studies were conducted for all the extracts against (Dalton Lymphocytes ascites) cells. The CTC50value was found to be 435 µg/ml. Thus, the extracts of P. Chinensis may possess cytotoxic, antioxidant and antimicrobial activity.

Keywords


Antimicrobial, DPPH Method, Cytotoxicity, Polygonum Chinensis, Short-Term Antitumor Studies.

References