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Molecular Diversity Assessment in Selected Accessions of White Seeded Sesame (Sesamum indicum L.) using SSR Markers


Affiliations
1 Biotechnology Section, JNKVV, College of Agriculture, Tikamgarh, Madhya Pradesh, India
2 Dept. of Genetics and Plant Breeding, ICAR-Directorate of Soybean Research, Indore, Madhya Pradesh, India
3 Agro-meteorology Section, JNKVV, College of Agriculture, Tikamgarh, Madhya Pradesh, India
4 Dept. of Crop Sciences, MGCGVV, Chitrakoot, Satna, Madhya Pradesh, India

Fifty sesame accessions with 10 simple sequence repeat (SSR) markers were used for their molecular characterization and assessment of genetic diversity. It was observed through this study that the accessions have enough genetic variability at molecular levels. Thirty five alleles with mean polymorphism information content of 0.42 resulted from the molecular studies very explicitly indicate the superiority of SSR primers in assessment of genetic diversity. These primer bands size varied from 200 to 400 bp. The number of alleles per locus in selected accessions varied from 3 to 6 and heterozygosity per primer ranged from 0.00 to 0.40. The pair wise genetic similarity varied from 0.44 to 0.86. A closure view of dendrogram identified two major clusters, indicating high genetic resemblance among sesame accessions. Hence, under the study here, diversity assessment through SSR markers was proved to be stronger tools for discriminating Sesamum indicum accessions.
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  • Molecular Diversity Assessment in Selected Accessions of White Seeded Sesame (Sesamum indicum L.) using SSR Markers

Abstract Views: 96  | 

Authors

Yogranjan
Biotechnology Section, JNKVV, College of Agriculture, Tikamgarh, Madhya Pradesh, India
G K Satpute
Dept. of Genetics and Plant Breeding, ICAR-Directorate of Soybean Research, Indore, Madhya Pradesh, India
Lalit M Bal
Biotechnology Section, JNKVV, College of Agriculture, Tikamgarh, Madhya Pradesh, India
A K Srivastava
Agro-meteorology Section, JNKVV, College of Agriculture, Tikamgarh, Madhya Pradesh, India
Sudhakar P Mishra
Dept. of Crop Sciences, MGCGVV, Chitrakoot, Satna, Madhya Pradesh, India

Abstract


Fifty sesame accessions with 10 simple sequence repeat (SSR) markers were used for their molecular characterization and assessment of genetic diversity. It was observed through this study that the accessions have enough genetic variability at molecular levels. Thirty five alleles with mean polymorphism information content of 0.42 resulted from the molecular studies very explicitly indicate the superiority of SSR primers in assessment of genetic diversity. These primer bands size varied from 200 to 400 bp. The number of alleles per locus in selected accessions varied from 3 to 6 and heterozygosity per primer ranged from 0.00 to 0.40. The pair wise genetic similarity varied from 0.44 to 0.86. A closure view of dendrogram identified two major clusters, indicating high genetic resemblance among sesame accessions. Hence, under the study here, diversity assessment through SSR markers was proved to be stronger tools for discriminating Sesamum indicum accessions.