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Enhanced Production of Tannase through RSM by Bacillus haynesii SSRY4 MN031245 under Submerged Fermentation


Affiliations
1 University Institute of Biotechnology, Chandigarh University, Gharuan, Mohali 140 413, Punjab, India
2 School of Engineering & Technology, Sharda University, Greater Noida 201 306, UP, India

Present investigation reports enhanced production of tannase by Bacillus haynesii SSRY4 MN031245 through application of Response Surface Methodology (RSM). Central Composite Design (CCD) of RSM was employed to determine the most important factors contributing to enzyme production and their interactions were analysed through graph models. Optimum production of enzyme (11.19 U/mL) was achieved at pH 5.5, temperature 37°C, incubation period 72 hours and agitation speed of 150.0 rpm. The statistically optimized values of variables enhanced the tannase production by 2.49 folds.
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  • Enhanced Production of Tannase through RSM by Bacillus haynesii SSRY4 MN031245 under Submerged Fermentation

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Authors

Sunny Dhiman
University Institute of Biotechnology, Chandigarh University, Gharuan, Mohali 140 413, Punjab, India
Gunjan Mukherjee
University Institute of Biotechnology, Chandigarh University, Gharuan, Mohali 140 413, Punjab, India
Anu Kumar
University Institute of Biotechnology, Chandigarh University, Gharuan, Mohali 140 413, Punjab, India
Rita Singh Majumdar
School of Engineering & Technology, Sharda University, Greater Noida 201 306, UP, India

Abstract


Present investigation reports enhanced production of tannase by Bacillus haynesii SSRY4 MN031245 through application of Response Surface Methodology (RSM). Central Composite Design (CCD) of RSM was employed to determine the most important factors contributing to enzyme production and their interactions were analysed through graph models. Optimum production of enzyme (11.19 U/mL) was achieved at pH 5.5, temperature 37°C, incubation period 72 hours and agitation speed of 150.0 rpm. The statistically optimized values of variables enhanced the tannase production by 2.49 folds.