Journal of Scientific and Industrial Research

Open Access Open Access  Restricted Access Subscription Access

Antioxidative Potential of Pollen, Propolis and Bee Bread against Damage Caused by Staphylococcus aureus in Liver and Kidney of BALB/c Mice: A Biochemical Study


Affiliations
1 Department of Biosciences (UIBT), Chandigarh University 140 413, Punjab, India
2 Department of Zoology, Punjab University, Chandigarh 160 014, India
 

Microbial resistance towards antibiotic is menacing all over the world and therefore considered to be a global health issue. Their potentialities for adapting new resistance mechanisms threaten our ability to treat common infectious diseases. Hence it is important to explore alternative therapies to combat microbial diseases. Among them, honeybee products comprise a wide variety of dietary/nutritional supplements with wide range of pharmaceutical properties. Therefore the present studies were designed to evaluate the therapeutic potential of propolis, pollen and bee bread against Staphylococcus aureus mediated oxidative stress in BALB/c mice. For this, different experimental groups were taken and decapitated at the end of experimental regimen. Liver and kidney were excised/homogenized and used for estimation of oxidative stress caused to them by following standard protocols. Observations revealed severe biochemical alterations by 5th day of infection. Among them, activity of antioxidant enzymes and reduced glutathione level were found to be decreased after S. aureus infection while the lipids peroxidation increased significantly in both liver and kidney. Bee products (250 mg/kg/bw of mice) restore the values of antioxidant enzymes/molecules and liver/kidney function enzymes to near normal by 30th day and results obtained were comparable with the positive control i.e. amoxicillin treatment group.

Keywords

Antibiotic Resistance, Antioxidative, Bee Bread, Lipids Peroxidation, Microbial Resistance.
User
Notifications
Font Size

  • Hiramatsu K, Katayama Y, Matsuo M, Sasaki T, Morimoto Y, Sekiguchi A & Baba T, Multi-drug-resistant Staphylococcus aureus and future chemotherapy, J Infect Chemother, 20 (2014) 593, doi:10.1016/j.jiac.2014.08.001.

  • Combarros-Fuertes P, Fresno J M, Estevinho M M, Sousa-Pimenta M, Tornadijo M E & Estevinho L M, Honey: Another alternative in the fight against antibiotic-resistant bacteria, Antibiotics, 9 (2020) 774, doi:10.3390/antibiotics9110774.

  • Loubet P, Ranfaing J, Dinh A, Dunyach-Remy C, Bernard L, Bruyère F, Lavigne J P & Sotto A, Alternative therapeutic options to antibiotics for the treatment of urinary tract infections, Front Microbiol, 11 (2020) 1509, doi: 10.3389/fmicb.2020.01509.

  • Al-Waili N, Al-Ghamdi A, Ansari M J, Al-Attal Y & Salom K, Synergistic effects of honey and propolis toward drug multi-resistant Staphylococcus aureus, Escherichia coli and Candida albicans isolates in single and polymicrobial cultures, Int J Med Sci, 9 (2012) 793–800, doi: 10.7150/ijms.4722, Epub 2012 Oct 26.

  • Boonsai P, Phuwapraisirisan P & Chanchao C, Antibacterial activity of a cardanol from Thai Apis mellifera propolis, Int J Med Sci, 11 (2014) 327–336, doi: 10.7150/ijms.7373.

  • Przybyłek I & Karpinski T M, Antibacterial properties of propolis, Molecules, 24 (2019) 2047, doi: 10.3390/molecules24112047.

  • Rana A & Kumar N R, Antioxidative potential of propolis on Staphylococcus aureus infected BALB/c mice: A biochemical study, Indian J Biochem Biophys, 59 (2022) 1006–1015.

  • Rana A, Kumar N R & Kaur J, Therapeutic effect of propolis on Staphylococcus aureus induced oxidative stress in kidney of BALB/c mice: a biochemical and histopathological study, Indian J Exp Biol, 60 (2022a) 597–606.

  • Rana A, Kumar N R & Kaur J, Therapeutic effect of propolis on Staphylococcus aureus induced oxidative stress in spleen of BALB/c mice: A biochemical and histopathological study, Indian J Nat Prod Resour, 13 (2022b) 1–13.

  • Rana A & Parmar A S, Re-exploring silver nanoparticles and its potential applications, Nanotechnol Environ Eng, (2022) 1–16, https://doi.org/10.1007/s41204-022-00301-w.

  • Rana A, Antibacterial, antifungal and antihelminthic properties of ethanolic, methanolic and water extracts of pollen, J Pharm Res Int, 33 (2021) 78, doi: 10.9734/jpri/2021/v33i53B33682.

  • Bakour M, Fernandes A, Barros L, Sokovi´c M, Ferreira I & Badiaa L, Bee bread as a functional product: Chemical composition and bioactive properties, LWT, 109 (2019) 276–282.

  • Kaškoniene V, Adaškeviciute V, Kaškonas P, Mickiene R & Maruška A, Antimicrobial and antioxidant activities of natural and fermented bee pollen, Food Biosci, 34 (2020) 100532.

  • Ayala A, Munoz M M & Argüelles S, Lipid Peroxidation: Production, metabolism and signaling mechanisms of malondialdehyde and 4-Hydroxy-2-Nonenal, Oxid Med Cell Longev, (2014), doi.org/10.1155/2014/360438.

  • Krishnamurthy P & Wadhwani A, Antioxidant Enzymes and Human Health, Antioxid Enzyme, 1 (2012) 3–18, doi:10.5772/48109.

  • Van-de-Sande-Bruinsma N & Fo-Wong D L, WHO European strategic action plan on antibiotic resistance: How to preserve antibiotics, J Pediatr Infect Dis, 9 (2014) 127.

  • Fischbach M A & Walsh C T, Antibiotics for emerging pathogens, Science, 325 (2009) 1089.

  • Wise R, BSAC working party on the urgent need: regenerating antibacterial drug discovery and development-the urgent need for new antibacterial agents, J Antimicrob Chemother, 66 (2011) 1939‒1940, doi: 10.1093/jac/dkr261. Epub 2011 Jun 23.

  • Wink M, Modes of action of herbal medicines and plant secondary metabolites, Medicines, 2 (2015) 251‒286, doi: 10.3390/medicines2030251.

  • Staerck C, Gastebois A, Vandeputte P, Calenda A, Larcher G, Gillmann L, Papon N, Bouchara J P & Fleury M J J, Microbial antioxidant defense enzymes, Microb Pathog, 110 (2017) 56‒65, doi: 10.1016/j.micpath.2017.06.015.

  • Hayyan M, Hashim M A & Al-Nashef I M, Superoxide Ion: Generation and Chemical Implications, Chem Rev, 116 (2016) 3029‒3085, doi: 10.1021/acs.chemrev.5b00407.

  • Mourenza Á, Gil J A, Mateos L M & Letek M, Oxidative stress-generating antimicrobials, a novel strategy to overcome antibacterial resistance, Antioxidants (Basel), 26 (2020) 361, doi: 10.3390/antiox9050361.

  • Nowogórska A & Patykowski J, Selected reactive oxygen species and antioxidant enzymes in common bean after Pseudomonas syringae pv. phaseolicola and Botrytis cinerea infection, Acta Physiol Plant, 37 (2015) 1725, doi.org/10.1007/s11738-014-1725-3.

  • Bollela V R, Namburete E I, Feliciano C S, Macheque D, Harrison L H, Caminero J A, Detection of katG and in hA mutations to guide isoniazid and ethionamide use for drug-resistant tuberculosis, Int J Tuberc Lung Dis, 20(8) (2016) 1099-104, doi: 10.5588/ijtld.15.0864.

  • Shastri M D, Shukla S D, Chong W C, Dua K, Peterson G M, Patel R P, Hansbro P M, Eri R & O’Toole R F, Role of oxidative stress in the pathology and management of human tuberculosis, Oxid Med Cel Longev, (2018) 7695364, doi: 10.1155/2018/7695364.

  • Ma Z, Strickland K T, Cherne M D, Sehanobish E, Rohde K H, Self W T & Davidson V L, The Rv2633c protein of Mycobacterium tuberculosis is a non-heme di-iron catalase with a possible role in defenses against oxidative stress, J Biol Chem, 293 (2018) 1590‒1595, doi: 10.1074/jbc.RA117.000421.

  • Bidaud P, He L, Sanguinetti M, Laugier C & Petry S, Rhodococcus equi’s extreme resistance to hydrogen peroxide is mainly conferred by one of its four catalase genes, PLoS ONE, 7 (2012) e42396, doi: 10.1371/journal.pone.0042396.

  • Staerck C, Gastebois A, Vandeputte P, Calenda A, Larcher G, Gillmann L, Papon N, Bouchara J P & Fleury M J J, Microbial antioxidant defense enzymes, Microb Pathog, 110 (2017) 56‒65, doi: 10.1016/j.micpath.2017.06.015.

  • Talwani R, Gilliam B L & Howell C, Infectious diseases and the liver, Clin Liver Dis, 15 (2011) 111‒130, doi:10.1016/j.cld.2010.09.002.

  • Bunchorntavakul C, Chamroonkul N & Chavalitdhamrong D, Bacterial infections in cirrhosis: A critical review and practical guidance, World J Hepatol, 8 (2016) 307‒321, doi.org/10.4254/wjh.v8.i6.307.

  • Malakouti M, Kataria A, Ali S K & Schenker S, Elevated liver enzymes in asymptomatic patients-What should I do, J Clin Transl Hepatol, 5 (2017) 394‒403, doi.org/10.14218/JCTH.2017.00027.

  • Oh T K, Jang E S & Song I A, Long-term mortality due to infection associated with elevated liver enzymes: a population-based cohort study, Sci Rep, 11 (2021) 12490, doi.org/10.1038/s41598-021-92033-1.

  • Minemura M, Tajiri K & Shimizu Y, Liver involvement in systemic infection, World J Hepatol, 6 (2014) 632‒642, doi:10.4254/wjh.v6.i9.632.

  • Gomaa M S, Alla M A A & Sameer M M, The possible protective effect of propolis (bee glue) on cypermethrin induced hepatotoxicity in adult albino rats, Mansoura J Forensic Med Clin Toxicol, 19 (2011) 17.

  • Parvez M K & Sakina N, Bacterial Infection of Liver: A Bird’s Eye View, J Gastroenterol Hepatol Res, 5 (2016) 2112, doi:10.17554/j.issn.2224-3992.2016.05.624.


Abstract Views: 56

PDF Views: 62




  • Antioxidative Potential of Pollen, Propolis and Bee Bread against Damage Caused by Staphylococcus aureus in Liver and Kidney of BALB/c Mice: A Biochemical Study

Abstract Views: 56  |  PDF Views: 62

Authors

Anita Rana
Department of Biosciences (UIBT), Chandigarh University 140 413, Punjab, India
Neelima R Kumar
Department of Zoology, Punjab University, Chandigarh 160 014, India

Abstract


Microbial resistance towards antibiotic is menacing all over the world and therefore considered to be a global health issue. Their potentialities for adapting new resistance mechanisms threaten our ability to treat common infectious diseases. Hence it is important to explore alternative therapies to combat microbial diseases. Among them, honeybee products comprise a wide variety of dietary/nutritional supplements with wide range of pharmaceutical properties. Therefore the present studies were designed to evaluate the therapeutic potential of propolis, pollen and bee bread against Staphylococcus aureus mediated oxidative stress in BALB/c mice. For this, different experimental groups were taken and decapitated at the end of experimental regimen. Liver and kidney were excised/homogenized and used for estimation of oxidative stress caused to them by following standard protocols. Observations revealed severe biochemical alterations by 5th day of infection. Among them, activity of antioxidant enzymes and reduced glutathione level were found to be decreased after S. aureus infection while the lipids peroxidation increased significantly in both liver and kidney. Bee products (250 mg/kg/bw of mice) restore the values of antioxidant enzymes/molecules and liver/kidney function enzymes to near normal by 30th day and results obtained were comparable with the positive control i.e. amoxicillin treatment group.

Keywords


Antibiotic Resistance, Antioxidative, Bee Bread, Lipids Peroxidation, Microbial Resistance.

References