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Ismail ¹, R. Rajavel ¹, M. Ganesh ¹, M. Jagadeeswaran ¹, K. Srinivasan ¹, J. Valarmathi ¹, T. Sivakumar ¹

Affiliations
1 Nandha College of Pharmacy, Division of Pharmaceutical Analysis, Koorapalayam Pirivu, Erode-638052, Tamil Nadu, IN

Abstract

A simple, precise, accurate and rapid HPLC method has been developed, and validated for the determination of Aspirin, Atorvastatin and Pioglitazone simultaneously, in combined dosage form. Acetonitrile and phosphate buffer with pH 3.5 (40%:60% v/v) is used as the mobile phase, 261nm is the detection wavelength for this study. The applicability of the method for simultaneous determination of Aspirin, Atorvastatin and Pioglitazone was verified by the determination of these compounds in marketed tablets. Results of the analysis were validated statistically, and by recovery studies (98.29-101.12%). The recovery and RSD values within the limits given in ICH guide lines method developments indicates that the suitability of proposed methods for the routine determination of these compounds in tablets. The validation parameters: linearity (r>0.996), sensitivity (LOD (1.5-0.7×10^-4 mg ml^-1 and LOQ%x10^-4-1.05×10^-4), accuracy (recoveries:98.29-101.12%)and reproducibility were found to satisfactory. The proposed method can be successfully used to determine the drug contents of marketed formulation.

Keywords

RP-HPLC, Aspirin, Pioglitazone, Atorvastatin.

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Ummu Balqis ¹, Muhammad Hambal ², Rinidar ³, Farida Athaillah ², Ismail , Azhar ⁴, Henni Vanda ³, Darmawi ⁵

Affiliations
1 Laboratory of Pathology, Syiah Kuala University, Banda Aceh, Aceh, ID
2 Laboratory of Parasitology, Syiah Kuala University, Banda Aceh, Aceh, ID
3 Laboratory of Pharmacology, Syiah Kuala University, Banda Aceh, Aceh, ID
4 Laboratory of Physiology, Syiah Kuala University, Banda Aceh, Aceh, ID
5 Laboratory of Research, Syiah Kuala University, Banda Aceh, Aceh, ID

Abstract

Aim: The objective of this research was to in vitro evaluate the cuticular surface damage of Ascaridia galli adult worms treated with ethanolic extract of betel nuts Veitchia merrillii.

Materials and Methods: Phytochemical screening was done using FeCl₃, Wagner and Dragendorff reagents, NaOH, MgHCl, and Liebermann–Burchard reaction test. Amount of 16 worms were segregated into four groups with three replicates. Four worms of each group submerged into phosphate buffered saline, 25 mg/ml, and 75 mg/ml crude ethanolic extract of V. merrillii, and 15 mg/ml albendazole. The effect of these extract was observed 40 h after incubation as soon as worms death. The worms were sectioned transversally and were explored for any cuticular histopathological changes in their body surface under microscope.

Results: We found that the ethanolic extract of V. merrillii betel nuts contains tannins, alkaloids, flavonoids, triterpenoids, and saponins. The ethanolic extract of betel nuts V. merrillii induces surface alterations caused cuticular damage of A. galli adult worms.

Conclusion: We concluded that ethanolic extract of betel nuts V. merrillii possess anthelmintic activity caused cuticular damage of A. galli adult worms.

Keywords

Anthelmintics, Ascaridia galli, Cuticle, Veitchia merrillii.

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