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Authors
Affiliations
1 Department of Biotechnology, Assam University, Silchar-788011, IN
2 College of Veterinary Science, Khanapara, Guwahati, Assam, IN
3 West Bengal University of Animal and Fishery Sciences, Kolkata-700037, IN
Source
Journal of Environment and Sociobiology, Vol 10, No 1 (2013), Pagination: 19-24
Abstract
The DNA barcoding of Felidae (cat family, using mitochondrial Cytochrome oxidase subunit-I (COI) region opens a new vista for molecular identification of different life forms on our planet and caters a boon for cataloguing of taxa. In the present study, an attempt is made to develop a set of universal primer (forward COI-Fl and reverse COI-R1) for PCR amplification of COI gene for higher mammals and validate by successful amplification and subsequent sequencing of the gene in Royal Bengal Tiger (Panthera tigris) and domestic cat (Felts sylvestris catus). The sequenced data are submitted in GenBank (Accession No FJ185309 and FJ185310) as DNA barcode of the species. The designed primers opened a new hope in mitigating a long standing problem of species identification due to poaching of wild animals, adulteration of meat and animal products and purity testing as it can amplify wide range of barcode sequences of higher mammals. The obtained sequences along with other relevant sequences already existed in global database are analyzed to determine phylogenetic relationship and discussed.
Keywords
DNA Barcoding, Royal Bengal Tiger, COI Gene, PCR, NCBI.
