- G. Senthilkumar
- A. Josphine
- P. Madhanraj
- K. Subha
- K. Kanimozhi
- S. Suganya
- R. Bharathidasan
- S. Manorajan
- N. Nadimuthu
- S. Deepa
- P. Bhuvanehswari
- K. Kaviyarasi
- A. Cholarajan
- A. Prathiba
- S. Gnanavel
- R. Mahalingam
- V. Ambikapathy
- A. Gayathri
- M. Prabakaran
- V. Thennarasu
- S. Gomathi
- K. Ramanathan
- N. Arun
- M. Mariyappan
- V. Saranya
- Research Journal of Pharmacology and Pharmacodynamics
- Research Journal of Science and Technology
- Asian Journal of Pharmaceutical Analysis
- Asian Journal of Pharmacy and Technology
- Asian Journal of Research in Pharmaceutical Sciences
- Research Journal of Pharmaceutical Dosage Form and Technology
- Asian Journal of Research in Chemistry
- Asian Journal of Pharmaceutical Research
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z All
Panneerselvam, A.
- Antibacterial Activity of Aspergillus oryzae against some Human Pathogens
Authors
1 Dept. of Botany and Microbiology, A.V.V.M Sri Pushpam College (Autonomous), Poondi, Thanjavur (Dt.) - 613 503, Tamil Nadu, IN
2 Dept. of Microbiology, Thanthai Hans Roever College of Arts and Science, Elambalur, Perambalur - 621 212, Tamil Nadu, IN
Source
Research Journal of Pharmacology and Pharmacodynamics, Vol 3, No 5 (2011), Pagination: 250-252Abstract
The marine fungi occurring on the wood samples were studied. Diversity of marine fungi colonizing the lignicolous wood samples was assessed and their distribution pattern was determined. The antibacterial activity of Aspergillus oryzae was tested against five human pathogens by agar well diffusion method. Based on the results, the compounds were separated by Thin Layer Chromatography (TLC) and these compounds were tested against three human bacterial pathogens. The separated compounds were further analyzed by Fourier Transform Infrared Spectroscopy (FTIR) to identify the functional groups, for the antibacterial activity.Keywords
Aspergillus oryzae, FTIR, TLC, Sterol.References
- Annette, N. J., 2009. A novel procedure for strain classification of fungal mycelium By cluster and artificial neural network analysis of Fourier Transform infrared(FTIR) spectra. Analyst.134: 1215-1223.
- Bugni. T.S., Ireland, C.M., 2004. Marine derived fungi a chemically and biologically diverse group of microorganisms. Nat. Prod. Rep. 21: 143-163.
- Korzybski,T., Kowszyk - Gindifer, and Kurylowicz., 1967. Antibiotics, Vol.2. Pergamon press, New York.
- Perez, C., Paul, M., Bazerque, P., 1990. Antibiotic assay by agar well diffusion method. Acta. Biol. Med. Exp., 15: 113-115.
- Singh, R., Sharma, M., Joshi, P., Rawat, D.S., 2008. Clinical status of anti-cancer agents derived from marine sources. Anticancer agents Med Chem. 8: 603-617.
- Wagner, R. and Bladt, S.D.,1996. Plant Drug Diagnosis, A thin layer chromatography, pp:384.
- Yanagihara, N., Sasaki-Takahashi, M., Sugahara, T., Yamamoto, S., Shinomi, M., Yamashita, E., Hayashida, M., Andoh, T., 2005. Leptosins isolated from marine fungus Leptosphaeraia species inhibit DNA topoisomerases I and/or II and induce apoptosis by inactivation of AKT/Protein Kinase B. Cancer. Sci. 96: 816-824.
- Young, X., Jun, L., An-guol., and Jing-Xia, C., 2003.Structure identification for the compound I separated and purified from taxoids produced endophytic fungi (Alternaria alternate var. taxi 1011) J.For.Res.14(3) : 217-233.
- Biodiversity of Fungi in Saltpan Soil from Coastal Area Vedaranyam, Nagapattinam District, Tamil Nadu
Authors
1 PG and Research Department of Botany and Microbiology, A.V.V.M Sri Pushpam College (Autonomous), Poondi 613 503, Thanjavur, Tamil Nadu, IN
2 PG and Research Department of Botany and Microbiology, A.V.V.M Sri Pushpam College (Autonomous), Poondi 613 503, Thanjavur, Tamil Nadu
Source
Research Journal of Science and Technology, Vol 5, No 2 (2013), Pagination: 250-254Abstract
In the present study soil sample were collected from salt pan region of Vedharanyam, Nagapattinam District, Tamilnadu. The physico-chemical characteristics of soil samples were found to affect the distribution of population of fungi. The fungal diversity from the soil sample were analysed by plating technique. Fungi isolation was done by the soil dilution method incubated at 26±2°C for 3-5 days. The predominant fungi isolated include; Asprgillus flavus, A. terreus, A. sydowi, A. oryzae, A. ruber, A. sulphureus, A. conicus, A. chevalieri, A. flavipes, Absidia glauca, Acrophialophora fusispora, Gliocladium sagariensis, Geotrichum candidum, Penicillium citrinum, P. chrysogenum, P. purpurogenum, Trichoderma harzianum, T. koningii, T. viride and Alternaria alternata.Keywords
Biodiversity, Fungi, Salt Pan Soil, Coastal, Physico-Chemical.- Antibacterial Activity of Essential Oil Extracted from Coriandrum sativam (L.) and GC-MS Analysis
Authors
1 PG and Research Department of Botany and Microbiology, A.V.V.M Sri Pushpam College (Autonomous), Poondi, Thanjavur, Tamil Nadu, IN
2 Dept of Microbiology, Thanthai Hans Roever College of Arts and Science, Perambalur - 621 212, Tamil Nadu, IN
Source
Research Journal of Science and Technology, Vol 4, No 5 (2012), Pagination: 203-207Abstract
Extraction of essential oils from locally available plant Coriandrum sativam (L.) was carried out using steam distillation followed by ether extraction. Dried and purified extracted oils were screened for their antibacterial activity of both gram positive (Staphylococcus aureus) and gram negative (Enterobacter aerogenes, Klebsiella pneumoniae, Vibrio cholerae and Salmonella typhi) bacterial strains by Agar well diffusion method. Coriandrum sativam shows antibacterial activity against the above given five bacterial strains. GC-MS was done on Coriandrum sativam oil. These results support that this plant oil can be used to cure bacterial infections and may also have role as pharmaceuticals and preservatives.Keywords
Coriandrum sativam, GC-MS, Essential Oil, Antibacterial Activity and Extraction.- Studies on the Fungi in Seasonally Inundated Sediment Habitat along South East Coast of India
Authors
1 Dept of Microbiology, Thanthai Hans Roever College of Arts and Science, Perambalur - 621 212, Tamil Nadu, IN
2 Biology Education Centre, Uppsala University, SE-752 36 Uppsala, SE
3 Dept. of Plant Science, Avvaiyar Government College for Women, Karaikal – 609 602, Pondicherry, U.T., IN
4 Dept. of Botany and Microbiology, A.V.V.M. Sri Pushpam College (Autonomous), Poondi-613 503, Thanjavur (Dt.), Tamilnadu, IN
Source
Research Journal of Science and Technology, Vol 3, No 4 (2011), Pagination: 175-179Abstract
In seasonally inundated sediments of Manianthivu of Nagapattinam district, southeast coast of India, a total number of 24 species belonged to 5 genera were recorded. They were assignable to Phycomycete (1 species) and Deuteromycetes (23 species). They exhibited spatio-temporal changes. The genus Aspergillus was constituted by the maximum of 19 species followed by Trichoderma (2 species). All other genera (Absidia, Botrytis and Fusarium) were represented by one species each. The soil characteristics such as pH (8.1 to 8.9), electrical conductivity (0.11 to 1.28 dSm-1), cation exchange capacity (8.01 to 9.5 c.mol proton+/kg), organic carbon (0.05 to 0.18%), available nitrogen (0.012 to 0.018%), available phosphorus (0.002 to 0.005%), available potassium (0.013 to 0.047 ppm), available zinc (0.39 to 0.57 ppm), available iron (2.12 to 2.83 ppm), available copper (0.19 to 0.31 ppm), available manganese (1.41 to 1.52 ppm), calcium (3.2 to 5.2 mg/kg), magnesium(3.2 to 3.9 mg/kg), sodium (0.52 to 1.22 mg/kg) and potassium (0.02 to 0.07 mg/kg) were also showed variation during different seasons. The investigation was carried out by collections and examination of sediment samples during January 2007 - December 2008, at seasonal intervals.Keywords
Fungi, Soil Characteristics, Inundated Sediment.- Isolation, Identification and Antibacterial Activity of Marine Actinomycetes Isolated from Saltpan Region
Authors
1 P.G. Research Dept. of Botany and Microbiology, A.V.V.M. Sri Pushpam College (Autonomous) Poondi-613503, Thanjavur Dist, Tamil Nadu, IN
2 P.G. Research Dept. of Botany and Microbiology, A.V.V.M. Sri Pushpam College (Autonomous) Poondi-613503, Thanjavur Dist, Tamil Nadu, IN
Source
Research Journal of Science and Technology, Vol 4, No 2 (2012), Pagination: 70-73Abstract
The soil sample was collected from the salt pan of Bay of Bengal at Vedaranyam,Nagapattinam (Dt.) Tamilnadu, India. The physico-chemical parameters at the sample were analyzed by using standard procedures.Totally 10 actinomycetes were isolated and identified from the salt pan soil sample. Two actinomycetes namely Jonesia denitrificans and Nocardia amarae were dominant in their growth out of 10 isolated actinomycetes.
Antibacterial activity of Jonesia denitrificans and Nocardia amarae were studied against five human bacterial pathogens such as Salmonella typhi, Bacillus subtilis, Staphylococcus aureus, Vibrio cholerae and Klebsiella pneumonia. The acetone extract of Nocardia amarae exhibited promising antibacterial activity against Bacillus subtilis (25 mm). The isolation, characterization and the study on actinomycetes can be useful in the discovery of antibiotics.
Keywords
Antibacterial Activity, Marine Actinomycetes, Jonesia denitrificans.- Utilization of Industrial Waste for the Production of Poly-Beta-Hydroxy Butyrate (Phb) by Alcaligenes eutrophus
Authors
1 PG and Research Department of Botany and Microbiology, A.V.V.M Sri Pushpam College, (Autonomous), Poondi, Thanjavur, IN
2 Department of Microbiology, A.V.C College (Autonomous), Mannampandal, Mayiladuthurai, IN
Source
Research Journal of Science and Technology, Vol 5, No 4 (2013), Pagination: 421-424Abstract
Alcaligenes eutrophus was collected from MTCC bank and purity was checked according to the guidelines of Bergey's manual of bacteriology. The PHB production ability of the culture was tested by "Granular stains method". The test organism was grown in synthetic and various industrial waste based medium (Molasses, seasame oil waste) at different concentration (10%, 20%, 30% and 40%).The PHB producer grown in synthetic and various industrial waste based medium and PHB was extracte and estimated by using UV spectrophotometer. The pure form of PHB powder was collected and studied by Infrared analysis. In the present study, the high PHB production was seen in Alcaligenes eutrophus grown in 40% concentration of seasame oil waste when compared with other substrate and synthetic medium. The present study concludes that the seasame oil waste based medium and Alcaligenes eutrophus used for PHB production in large scale that minimize the cost of the product. Thus we can minimize the use of petroleum-derived plastics and by which we can live in a plastic pollution free earth.Keywords
PHB, Molasses, Seasame Oil Waste, Alcaligenes eutrophus.- Effect of PGPR Studies on Some Angiospermic Plants
Authors
1 P.G. and Research Department of Botany and Microbiology, A.V.V.M Sri Pushpam College (Autonomous), Poondi -613503, Thanjavur, Tamilnadu, IN
2 P.G. and Research Department of Botany and Microbiology, A.V.V.M Sri Pushpam College (Autonomous), Poondi -613503, Thanjavur, Tamilnadu, IN
Source
Research Journal of Science and Technology, Vol 3, No 2 (2011), Pagination: 76-79Abstract
PGPR offers an environmentally sustainable approach to crop production and health. Many members of genera pseudomonas sp. perform beneficial effects on plants. Among them P. putida and P. fluorescens are fluorescent pseudomonas that represent Significant part of these Members. The rhizosphere soil was collected from cultivated plots. P. fluorescence was isolated and identified through morphologicaland hemical tests and it was treated against some plant pathogens. The biocontrol potential of P. fluorescens against plant pathogen such as A. terreus sp, Circinella sp, Verticillium sp, was assessed by dual plate technique. The seed treatment was made on four plants namely Hibiscus esculentus, Oryza sativa, Capsicum annum, Spinacia olerace. Pot culture techniques was applied for the growth of plants along with p. fluorescence inoculums (100ml) and chemical fertilizer (10g).The plant height, ischolar_main length, ischolar_main biomass, shoot biomass were also estimated. The results were discussed.Keywords
PGPR, Pseudomonas fluorescence.- Studies on Morphological, Physiological and Biochemical Characterization Azospirillum Spp
Authors
1 Dept of Botany and Microbiology, A. V. V. M. Sri Pushpam College (Autonomous), Poondi- 613 503, Thanjavur Dt , Tamilnadu, IN
Source
Research Journal of Science and Technology, Vol 3, No 2 (2011), Pagination: 85-94Abstract
Asymbiotic, associative, or symbiotic, Nitrogen fixing strains of Azospirillum isolated from Rhizosphere soils of paddy field. Totally 30 Azospirillum strains were isolated from paddy field of Thanjavur District by using Nitrogen free bromothymol blue (Nfb) medium. The isolated strains were characterized by physiological, morphological and biochemical characteristics and construct the phylogenetic tree. Based on this characterization, among the 30 isolates, nine belonged to A. halopraferens, 8 belonged to A. amazonense, 6 belonged to A. brasilense, 4 belonged to A. lipoferm and 3 belonged to A. irakense.Keywords
Morphological, Physiological, Biochemical Character, Azospirillum Spp.- Cultivation, Composting, Biochemical and Molecular Characterization of Pleurotus platypus (Cooke and Massee) Sacc
Authors
1 Department of Botany and Microbiology, A. V. V. M Sri Pushpam College, Poondi – 613 503, Thanjavur District, Tamil Nadu, IN
2 Department of Botany and Microbiology, A. V. V. M Sri Pushpam College, Poondi – 613 503, Thanjavur District, Tamil Nadu., IN
Source
Research Journal of Science and Technology, Vol 3, No 5 (2011), Pagination: 284-287Abstract
In the present investigation, the mushroom yield, chemical composition, nutritional value of Pleurotus platypus cultivated in paddy straw, black gram stem and black gram pods were determined. Fresh mushroom yield amounts obtained from paddy straw, black gram stem and black gram pods substrate were 940, 560 and 595 g / kg respectively. Mushroom cultivated on different substrates were analyzed for protein, carbohydrate, amino acids and fatty acid content. The cellulolytic activity and coir pith compost was also investigated in Pleurotus platypus. Hence P. platypus was also selected for molecular studies. Therefore, the present results suggest it could be used to recycle the agro – waste materials.Keywords
Pleurotus platypus, Chemical Composition, Nutritive Value, Cellulolytic Activity, Coir Pith Compost.- Diversity of Fungi from Drift Wood of Muthupet Mangroves
Authors
1 Department of Botany and Microbiology, A.V.V.M. Sri Pushpam College [Autonomous], Poondi-Thanjavur, IN
2 Dept of Microbiology, Thanthai Hans Roever College of Arts and Science, Perambalur - 621 212, Tamil Nadu, IN
Source
Asian Journal of Pharmaceutical Analysis, Vol 1, No 3 (2011), Pagination: 53-55Abstract
The variety and galaxy of fungi and their natural beauty occupy prime place in the biological world and India has been the cradle for such fungi. Only a fraction of total fungal wealth has been subjected to scientific scrutiny and mycologist have to unravel the unexplored and hidden wealth. The marine fungi occurring on the wood samples collected from the inter tidal region of the Muthupet mangrove environment were studied. Diversity and distribution of different organisms in the marine environment are influenced by the physico-chemical properties of the soil. Totally 23 fungal species were recorded.Keywords
Fungi, Diversity, Wood Samples, Mangroves.- Antimicrobial Activity of Strychnos nux-vomica Linn and Cassia angustifolia Linn
Authors
1 BG and Research Dept of Botany and Microbiology, A.V.V.M. Sri Pushpam College (Autonomous), Poondi - 613 503, Thanjavur, Tamil Nadu, IN
2 Dept. of Microbiology, Thanthai Hans Roever College of Arts and Science, Perambalur - 621 212, Tamil Nadu, IN
Source
Asian Journal of Pharmacy and Technology, Vol 2, No 1 (2012), Pagination: 8-11Abstract
The antimicrobial effect of some selected Indian medicinal plants Strychnos nux-vomica and Cassia angustifolia, were selected. The solvents used for the extraction of plant leaves were n-butanol, methanol and distilled water. The invitro antimicrobial activity was performed by agar well diffusion method. The most susceptible gram-positive bacteria was Staphylococcus and Salmonella while other most susceptible gram negative bacteria was klebsiella pneumonia and fungal species namely Aspergillus terreus, Aspergillus flavus and Aspergillus niger. The n-butanol extract of plant Strychnos nux-vomica and Cassia angustifolia, inhibit all the bacterial and fungal strains investigated. The most active extract was compared with the standard antibiotics, penicillin, streptomycin and ampicillin with 100mg disc. In the present study Strychnos nuxvoimica and Cassia angustifolia showed maximum inhibition against the fungal and bacterial organisms tested. Thus the n-butanol plant extracts could be used to control the above microbes.- Isolation, Identification and Molecular Characterization of Phosphate Solubilizing Actinomycetes Isolated From The Coastal Region of Manora, Thanjavur (Dt.)
Authors
1 Department of Botany and Microbiology, A.V.V.M .Sri Puspham College [Autonomous], Poondi-613 03, Thanjavur, Tamil Nadu, IN
2 Dept of Microbiology, Thanthai Hans Roever College of Arts and Science, Perambalur-621 212, Tamil Nadu, IN
Source
Asian Journal of Pharmacy and Technology, Vol 1, No 4 (2011), Pagination: 119-122Abstract
The soil sample was collected from Manora. After analysing the physic-chemical parameters of the collected marine soil sample, ten actinomycetes species including Actinobispora yunnanensis, Streptomyces albus,Micromonospora echinospora, Saccharopolyspora hirsute, Streptomycetes cyaneus, Actinomadura citrea, Saccharomonospora viridis, Thermomonospora mesophila, Streptoverticillium album Microtetrospora fastidiosa were isolated and they were screened for phosphate solubilization using Pikovskaya's agar medium and the Actinobispora yunnanensis was found to be dominant in phosphate solubilization. Then the DNA of the Actinobispora yunnanensis was isolated, amplified and its 16S rDNA was sequenced and its secondary structure was also predicted.The phylogenetic analysis of the Actinobispora yunnanensis was also carried out.Keywords
Phosphate, Phosphate Solubilization, 16S r DNA, Actinobispora yunnanensis.- Diversity, Antibacterial Activity And Molecular Characterization of Actinomycetes Isolated From Salt Pan Region of Kodiakarai, Nagapattinam DT.
Authors
1 Department of Botany and Microbiology, A. V. V. M. Sri Pushpam College [Autonomous], Poondi-Thanjavur, IN
2 Dept of Microbiology, Thanthai Hans Roever College of Arts and Science, Perambalur - 621 212, Tamil Nadu, IN
Source
Asian Journal of Pharmacy and Technology, Vol 1, No 3 (2011), Pagination: 79-81Abstract
The present study was to isolate and identify antimicrobial activity producing actinomycetes strains from marine habitat, to find out antibacterial efficiency against the common human pathogens. Marine actinomycetes were isolated from salt pan region of Kodiakarai, Nagapattinam [District].Out of 20 isolated actinomycetes 10 were identified and selected for antibacterial activity. Out of 10 dominant actinomycete species Streptoverticillium album was highly dominant and showed the best level of antibacterial activity against three human pathogens, Staphylococcus aureus, Klebsiella pheumoniae and Escherichia coli. The isolation, characterization and the study on actinomycetes can be useful in the discovery of antibiotics and also confirmed by molecular characterization.Keywords
Antibacterial Activity, Sterptoverticillium album, Marine Actinomycetes.- Screening of Antioxidant, Antimutagenic, Antimicrobial Activities and Phytochemical Studies on Sphaeranthus amaranthoides (Burm)
Authors
1 Sri Gowri Biotech Research Academy, No.5 Nagai Road, Thanjavur, 613 001, Tamilnadu, IN
2 Dept. of Botany and Microbiology, A.V.V.M. Sri Pushpam College (Autonomous), Poondi 613 503, Tamilnadu, IN
Source
Asian Journal of Pharmacy and Technology, Vol 1, No 4 (2011), Pagination: 125-129Abstract
The learn of Sphaeranthus amaranthoides Burn, were examined for their antioxidant, antimutagenic, antimicrobial and phytochemical activities. Preliminary evaluation of both crude and solvent fractions showed a broad spectrum of activity since the extracts inhibit the growth of both bacterial and fungal isolates. The inhibitory activity of plant extracts on the peroxidation of linolic acid were measured by fericthiocynate methodo. Ethonolic extract showed strong antioxidant activity and antimicrobial activity against to the bacteria, (K.pneumoniae, S. typhi, E.coli, P.aeroginosa, S.aureus) and fungus (A.nidulans, A.terreus, A.sulphureus, Candida albicans and Fusarium sp.) cultures. Due to the phytochemical diversity in the antioxidant phytocompounds, the above results were expected. It almost rich in carotenes, Neoxanthin, chlorophyll a and chlorophyll b, lutein, violexanthin, pheophytin, which may have been responsible for the antioxidant, antimicrobial and for phytochemical activity. For antimutagenic activity using sodium azide to induce the chemical compound of Sphaeranthus amaranthoides Burn, to prevent the degenerative cells and results further support the view that some Indian medicinal plants are source for potential antioxidants.Keywords
Medicinal Plants, Ferric Thiocyanate, Sodium Azide, Solvents, Human Pathogens, Thin Layer Chromatography.- Studies on Soil Mycoflora in Chilli Field of Thiruvarur District
Authors
1 P.G. and Research Department of Botany and Microbiology, A.V.V.M. Sri Pushpam College (Autonomous), Poondi, Thanjavur (Dt)- 613503, Tamil Nadu, IN
Source
Asian Journal of Research in Pharmaceutical Sciences, Vol 1, No 4 (2011), Pagination: 117-122Abstract
Soil is a complex and dynamic environment in which the biological activity is mostly governed by microorganisms. This study deals with the monthly variation of fungal population in chilli field of four different taluk Thiruvarur, Nannilam, Kudavasal and Valangaiman of Thiruvarur(Dt). In the present investigation 40 different species belonging to Deuteromycetes, Ascomycetes and Phycomycetes were isolated using PDA medium and identification by using standard manuals. The maximum number of fungal isolates were recorded in valangaiman (20) when compared to other stations. The dominant species recorded were Aspergillus and Penicillium.Keywords
Fungal Diversity, Chilli Field, Thiruvarur District.- Studies on the Compound Separation and Its Antifungal Potentiality of Fungi Isolated From Nagapattinam District, East Coast of India
Authors
1 Dept. of Botany and Microbiology, A.V.V.M. Sri Pushpam College (Autonomous), Poondi- 613 503, Thanjavur Dt., Tamil Nadu, IN
2 Dept of Botany and Microbiology, A.V.V.M Sri Pushpam College (Autonomous), Poondi, Thanjavur (Dt) – 613 503, Tamil Nadu, IN
Source
Research Journal of Pharmaceutical Dosage Form and Technology, Vol 3, No 3 (2011), Pagination: 100-104Abstract
Altogether 37 species belonged to 10 genera were recorded. A preliminary screening of all the species isolated from soils were used for antifungal (antagonistic) activity against Fusarium semitectum, a known soil borne fungal pathogen. Among the species tested, Trichoderma koeningii showed promising activity, inhibited the pathogenic fungus for the maximum both in dual culture and in food poisoning technique. When HPLC fractionation of the mycelial extract of T. koeningii grown in PDA broth yielded three fractions. They were named as peak 1, peak 2 and peak 3.Among the three fractions, peak 2 alone showed the antifungal activity with the inhibition zone of 13 mm diameter against Fusarium semitectum. The functional groups present in peak 1 and 3 are almost same, but in the peak 2 some extra functional groups are present. These groups may be responsible for the antifungal activity of this fraction.
Keywords
Antifungal Activity, Dual Culture and Food Poisoning Technique, HPLC, Compounds, Biocontrol.- Studies on DNA Extraction, Molecular Identification and Genetic Evolution of Trichoderma harzianum
Authors
1 Dept of Botany and Microbiology, A. V. V. M Sri Pushpam College (Autonomous), Poondi, Thanjavur (Dt) - 613 503, Tamil Nadu, IN
2 Dept of Microbiology, Thanthai Hans Roever College of Arts and Science, Perambalur - 621 212, Tamil Nadu, IN
Source
Asian Journal of Research in Chemistry, Vol 4, No 8 (2011), Pagination: 1225-1230Abstract
The chromosomal DNA was isolated from T. harzianum and PCR amplification was performed. The 18S rDNA gene sequencing was made by using ITS (internal transcribed spacer) method. The 18S rDNA gene sequences were submitted to Genebank, NCBI, the accession number GU646678 has been obtained. The phylogenetic tree was constructed using MEGA 4 (Molecular Evolutionary Genetics Analysis Software version 4.0). Based on the neighbour joining tree analysis the isolate T. harzianum has been found closely related to already existing species of Hypocera tixii at 99 per cent of similarity.Keywords
Trichoderma harzianum, DNA Isolation, Evolution.- Atomic Study on Fusarium incarnatum using Bioinformatics Tools
Authors
1 Department of Microbiology, Thanthai Hans Roever College, Perambalur-621212, IN
2 Department of Bioinformatics, Thanthai Hans Roever College, Perambalur-621212, IN
3 Department of Biochemistry, Thanthai Hans Roever College, Perambalur-621212, IN
4 Dept. of Plant Science, Avvaiyar Govt. College for Women, Karaikal-609602, Puducherry, U.T., IN
5 Department of Botany and Microbiology, A.V.V.M Sri Puspam College, Poondi, Thanjavur–613503, Tamil Nadu, IN
Source
Asian Journal of Pharmaceutical Research, Vol 2, No 2 (2012), Pagination: 78-82Abstract
We have been investigating that the atomic study of Fusarium incarnatum with the utilization of Bioinformatics tools. We have been involved in the insilico study on the plant pathogen the Fusarium incarnatum. The involvement of Nucleotides and atoms in this species can be identified with the help of Bioinformatics tools. We can also involved in the analysis of amino acids which are present in this species and calculated the start and stop codons in different frames.Keywords
Fusarium incarnatum, Bioinformatics Tools, Nucleotides, Atomic Study, Amino Acids, Frame Analysis.- Antibacterial Activity of Cardiospermum halicacabum and Melothria heterophylla
Authors
1 P.G.Research Dept. of Botany and Microbiology, A.V.V.M. Sri Pushpam College (Autonomous), Poondi-613503, Thanjavur. DT, Tamil Nadu, IN
2 Dept of Microbiology, Thanthai Hans Roever College of Arts and Science, Perambalur-621212, Tamil Nadu, IN
Source
Asian Journal of Pharmaceutical Research, Vol 1, No 4 (2011), Pagination: 111-113Abstract
The bacterial organisms were isolated from drinking water (Bacillus, Borchothrix, Clavibacter sp, Ancylobacter sp, and Brevi bacterium). Selected Indian medicinal plants Cardiospermum halicacabum and Melothria heterophylla were selected for antibacterial studies. The solvents used for the extraction of plant ischolar_mains were n- butanol, ethyl acetate and distilled water. The invitro antibacterial activity was performed by agar well diffusion method. The most susceptible Gram-Postive bacteria was Bacillus sp, Brevibacterium sp, and the most susceptible Gram-negative bacteria was Borchothrix sp, Clavibacter sp, and Ancylobacter sp. The extracts of plant Cardiospermum halicacabum and Melothria heterophylla inhibited the growth of the bacterial strains investigated. The most active extracts was compared with the standard antibiotics, penicillin, Streptomycin and Ampicillin 100mg/disc). The results obtained in the present study suggest that Cardiospermum halicacabum and Melothria heterophylla could be used in treating diseases caused by the test organisms. The results are discussed in detail.Keywords
Medicinal Plants, Antibacterial Activity, Aqueous Extract, N-Butanol Excretory Ethyalcetate Extract.- Cultivation, Composting, Biochemical and Molecular Characterization of Calocybe indica (C and A)
Authors
1 Department of Botany and Microbiology, A.V.V.M. Sri Pushpam College [Autonomous], Poondi, Thanjavur, IN
2 Dept of Microbiology, Thanthai Hans Roever College of Arts and Science, Perambalur-621212, Tamil Nadu, IN
Source
Asian Journal of Pharmaceutical Research, Vol 1, No 3 (2011), Pagination: 55-57Abstract
Investigation on the cultivation of milky mushroom, Calocybe indica was conducted to find out the growth and yield performance on different substrates. The yield of fresh mushroom obtained from paddy straw, teak leaves and sugarcane trash substrates were 1140, 745 and 570 g/kg respectively. The nutritional significance of Calocybe indica cultivated on different substrates was assessed in terms of protein, carbohydrate, amino acids and lipids. The cellulolytic activity and coir pith compost was also tested using Calocybe indica. The present result suggest, the lignocellulosic substrate like paddy straw, teak leaves and sugarcane trash shown great potential for use as a raw material since this substrate provides an economically acceptable production for Calocybe indica.Keywords
Calocybe indica, Sugarcane Trash, Teak Leaves, Composting of Coir Pith.- Mycoecology in the Soil of a Casuarina Bioshield Plantation along Southeast Coast of India
Authors
1 Dept. of Microbiology, Thanthai Hans Roever College of Arts and Science, Perambalur, Tamil Nadu, IN
2 Dept. of Plant Science, Avvaiyar Govt. College for Women, Karaikal-609602, Puducherry, U.T., IN
3 P.G. and Research Dept. of Botany and Microbiology, A.V.V.M. Sri Pushpam College (Autonomous), Poondi-613503, Thanjavur (Dt.), Tamilnadu, IN
Source
Asian Journal of Pharmaceutical Research, Vol 1, No 2 (2011), Pagination: 37-41Abstract
A total number of 325 colonies were isolated by plating method from the soils of Casurina bioshield plantation at Naluvedhapathy (Nagapattinam Dt.), along south east coast of India. There were 19 species belonged to 4 genera with Aspergillus as the dominant genus, represented by more number of species (14 species), followed by Fusarium and Trichoderma (2 species each). Population density varied from 9.7 to 17.7 CFU/g and the species diversity from 6 to 10 species during different stations. Physico-chemical analyses of the soils revealed the following features: pH 7.9 to 8.8, electrical conductivity 1.06 to 1.31 dSm-1, cation exchange capacity 8.67 to 10.11 c.mol proton+/kg, organic carbon 0.04 to 0.21%, available nitrogen 0.011 to 0.022%, available phosphorus 0.002 to 0.005%, available potassium 0.013 to 0.051 ppm, available zinc 0.47 to 0.57 ppm, available iron 2.13 to 2.90 ppm, available copper 0.22 to 0.31 ppm, available manganese 1.38 to 1.53 ppm, calcium 3.1 to 5.1 mg/kg, magnesium 3.2 to 4.1 mg/kg, sodium 0.23 to 0.79 mg/kg and potassium 0.02 to 0.08 mg/kg, during different seasons.Keywords
Soil Fungi, Bioshield Plantation, Soil Characteristics, Mycoecology.- Studies on the Compounds and its Antifungal Potentiality of Fungi Isolated from Paddy Field Soils of Jenbagapuram Village, Thanjavur District, and South India
Authors
1 Dept of Botany and Microbiology, A.V.V.M Sri Pushpam College (Autonomous), Poondi-613503, Thanjavur (Dt), Tamil Nadu, IN